聚焦

By extending the concept of MBE technique and employing the uniform buffers to separating the sample electrophoresis, the zone electrophoresis was developed. The isotachoporesis technique is based on the separation in a discontinuity form between two buffer solutions and results in separation of samples. The isoelectric focusing is a method that concentrates the ampholyte sample by the pH gradient introduced in the background ampholytes.

区间电泳法以移动边界电泳法作为基础的技术，其在系统环境充满均匀的缓冲液进行分离；等速电泳法则利用两缓冲液进行不连续分离之技术；等电点聚焦电泳法则利用背景溶液建立出pH值梯度，双性电解液样品因其位於等电点所受库伦力为零而聚焦於此。

Starting with the earlier studies on point of view, this paper elaborates on the studies of and the debates about focalization in the context of classical narratology and explores the current state of focalization studies in the context of postclassical narratology with cases like feminist narratology and cognitive narratology.

从早期的叙述视角研究谈起，详细评析经典叙事学视阈下叙述聚焦理论的建构与论争。同时，以女性主义叙事学和认知叙事学为例，着力探究后经典叙事学视阈下叙述聚焦研究的新态势，希图从全局上考察和把握叙述聚焦研究在西方学界的嬗变。

Based on the aberration theory of AWG, a new-type flat-field AWG is designed. The focal signals of all wavelengths of operation are focusing along a straight line perpendicular to the propagation direction. Three stigmatic points restrain three dominant geometry parameters. Furthermore, a new-type flat-field wavelength router with both flat input field and flat focal field is also designed.

基于阵列波导光栅的像差理论，利用三个无像差点约束三个主要结构参量，设计了新型平场聚焦阵列波导光栅，使得所有工作波长的输出信号都聚焦在与光束传输方向垂直的直线上，并进一步将其拓展为同时具有平输入场和平聚焦场的新型平场波长路由器。

The design was optimized for maximum signal intensity by simulation and experiments. The size of focal spot in the micro-channel capillary was optimized. Fluorescence signal was enhanced 2～3 times when increasing the diameter of focal spot from 10μm to 50μm (full of the inner wall of capillary), through which the sensitivity and signal-noise ratio were also improved. A technique of using refractive matching liquid was presented. The fluorescence signals were boosted up and the signal collection efficiency was also meliorated when using this method.

通过理论分析和实验优化本系统的设计：优化聚焦于微通道中的激发光斑大小，增大聚焦到毛细管内径中的激发光斑尺寸，使光斑尺寸由最小的10μm增大到50μm，光电倍增管接收到的荧光信号提高了2-3倍，从而提高系统的信噪比和灵敏度；提出在聚焦物镜处加折射率匹配液的方法，加匹配液后的荧光信号得到了增强，因而提高了荧光收集效率和灵敏度。

After comparing nonlinear simulation with linear, the results indicate that: Weak nonlinearity has definite and apparent influence on the distribution of acoustic fields and focused gain of array, which cause the distribution of waveform is closer to the array and the focused gain deteriorate ; the strong nonlinearity have a strong waveform aberration, because the other waves of non-fundamental frequency appear . No evident distinction is found in the effects between the oblique propagation and the vertical propagation, though less focused gain and influence caused by strong nonlinear produced by the acoustic diffraction which results in more attenuation in terms of oblique propagation.

将线性条件下的模拟结果与非线性条件下的模拟结果进行比较发现：弱非线性会对声场的分布和阵列聚焦增益产生一定的影响，使得声场分布波形比线性条件下的更加靠近阵列，聚焦效果变差；强非线性会使波形发生更严重畸变，这是由于产生了基频以外的其他频率声波引起的；非线性对斜向传播时声场分布的影响与垂直传播时的影响效果基本相同，但由于斜向辐射时的声波衍射造成的衰减要大于垂直辐射时的，因此聚焦增益和强非线性的影响都将小于垂直辐射时的情况。

We use GRIN lens as collimating or focusing lens for transmission gas cell ,and a method that using cascaded GRINLens couple to increase the optical path length is presented. Using this method ,the detection sensitivity of the gas cell has been greatly increased.

利用自聚焦透镜作为光纤准直透镜和聚焦透镜，应用于透射型气室，并提出了在透射式气室中将多对自聚焦透镜组串联用以增加吸收光程的方法，用该方法可以明显提高气室的检测灵敏度。

METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞，运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化；用gp120处理并用anti-gp120-FITC进行染色，运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况；用抗磷酸化ERK 抗体免疫荧光方法进行染色，运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

First, second and third position sensor controllers control the focus servo to focus the He--Ne laser beam on the glass substrate in response to the pit width of the glass substrate by sensing the laser beam for focusing reflectedupon the glass substrate.

此聚焦系统利用第一、第二、第三光点位置敏感器去控制氦一氖雷射，使其能够在玻璃基板上产生正确的凹坑宽度，而第一、第二、第三光点位置敏感器即藉著聚焦在玻璃基板上反射而回的氦一氖雷射作为聚焦控制。

Based on the relationship between the numerical aperture of the objective lens and scale pitch, this study chooses a suitable Plano-convex lenses available on the market, so as to approximate the diameter of the focusing spots to scale pitch. Also, by using the optical path of an optical encoder similar to the nanoscale, the changes in the surface profile of the scale can be measured directly, so as to obtain the two quadrature sinusoidal signals. By counting the zero crossing of these two quadrature sinusoidal signals and calculating the interpolation value of these two quadrature sinusoidal signals, the moving direction and moving displacement value of the scale can be obtained.

研究中再以搭配物镜的数值孔径与尺条间距的关系，选择ㄧ适当的市售平凸透镜，使聚焦光点的直径与尺条的间距相近，并使用相同於奈米级长行程双雷射聚焦读头装置的光路设计，如此将可藉由直接量测尺条的表面轮廓变化，以获得一组正交弦波讯号，继而利用讯号四分割与细分割处理，计算出尺条之移动方向与移动量，研制出一实用性长行程双雷射聚焦读头光学尺。

The seed purity of more than one hundred and thirty samples of six maizevarieties was assayed by using the three testing methods: storage proteins electrophoresis,isozyme isoelectric focusing in acrylamide and examination at field plots. Some importantpoints can be concluded from analyses as spearman order rank correlation on the seedpurity data as follows:First, there were deviations of the real performances to those purities obtained from out or in laboratory testing, even from the examination at field plot that is a routine way. Second, the seed globulin and isozyme electrophoresis, both with characteristics of rapid, cost-effective, devoid of environmental effect and speed, showed consistent purity; while the field-testing gave a higher degree of purity on the high side of cost than that in-lab approaches. Third, a good consistent purity was observed in the field plot and seed globulin testing, whereas inconsistency between field-testing and isozyme process. The last point is that seed globulin electrophoresis be a practical method apt to seed purity testing for maize.

并对130多份样品用三种检验方法(盐溶蛋白电泳、同功酶等电聚焦电泳和田间小区)检测的种子纯度数据作排序相关等统计分析，得到如下结果：室内外检测方法都会与种子真实状况有一定差异，田间种植鉴定与真实情况符合性并不一定最好；盐溶蛋白与同功酶等电聚焦这两种方法检测种子纯度所需时间短，成本低，不受外界环境限制，出结果快，二者的结果没有太大差异；田间种植鉴定成本较高，检测的结果普遍偏高；等电聚焦电泳与田间鉴定的一致性年度间存在差异，而盐溶蛋白凝胶电泳法与田间鉴定的一致性较高；种子盐溶蛋白凝胶电泳法较适于鉴定玉米种子纯度。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。