聚焦

This means that although they are more focusable than a hand torch say, the size of spot they can be focused to is still relatively large, limiting their use.

但高能量的激光二极管不能产生完美的激光束，这意味着他们虽然比手电筒更有聚焦能力，它们能聚焦的光斑尺寸仍然相对很大，从而限制它们的用途。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

The collimation and focus systems discussed in this paper are the important components of the synchronal radiation equipment which can change the direction of X ray and focus it.

同步辐射是生物大分子研究中所使用的重要方法，本文讨论的准直镜和聚焦镜系统是生物大分子光束线中的重要装置，其作用是改变X射线的方向并使其聚焦。

If a person is short-sighted,images are focused in front of their retina,and if a person is long-sighted,images are focused beyond their retina.

人如果近视的话，图像就会聚焦在视网膜前方，如果远视的话，图像就会聚焦在视网膜之后。

In this paper, we proposed a new method of coupling in terms of the request of the intersatellite optical communication system. Using an aspherical cylindrical lens whose front cylindrical lens is orthonormal to its back cylindrical lens collimates the laser beam, and then is focused by a GRIN lens and couples to the single-mode fiber.

针对星间光通信系统的要求，本文采用一种新型的半导体激光耦合方案，用前后正交的非球面柱面透镜准直半导体激光束，再经渐变折射率自聚焦透镜聚焦耦合入单模光纤。

The third chapter elaborates from the narrative angle of view, Eliot's novels passed through the route from by "focusing" the male to "focusing" the female, which partially demonstrated the feminine authority, however the novel presented the tendency of desalinating the feminine perspective unceasingly, which partially dispersed the feminine authority.

第三章从叙事视角展开论述，爱略特前后期的小说走过了从以&聚焦&男性到以&聚焦&女性为主的视角路线，部分地显示了女性权威，然而小说又呈现不断地淡化女性视角的趋势，又部分地消解了女性权威。

By focusing and harnessing all your power, energy, time, focus, thinking, etc.

聚焦和控制你所有的力量，精力，时间，聚焦，思考等等。

Mobile positioning system or dynamic table so that the laser head is cutting material in the system-driven subcapital high speed, laser head from the light source and cutting heads, light source produces a very short wavelength focused laser beam, laser beam through cutting heads, vertically focused the material being cut on the surface, heating and melting, evaporation is cutting material, resulting in the closure of Lancing, slotted openings formation pads.

移动定位系统妪动工作台或激光头，使得被切割材料在系统驱动头下高速运动，激光头由光源部分和切割头组成，光源部分产生波长很短的聚焦激光束，激光束通过切割头，垂直聚焦在被切割的材料表面上，加热、融化、蒸发被切割材料，形成切缝，闭合的切缝形成焊盘开孔。

In a camera, light rays pass through a series of lenses that focus images onto film.In a healthy eye, light rays pass through the cornea and lens, which focuses images onto the retina, a layer of light-sensing cells lining the back of the eye.

在照相机中，光线经过一系列的透镜，把图像聚焦在胶片上，在健康的眼睛中，光线通过角膜和透镜，把图像聚焦到视网膜上，而视网膜是一层排列在眼底的光敏单元。

Ten one underwater images of the micro actuator working at 42℃ have been used to illustrate the idea of measuring the tip deflection using optical focus method.

实验中，使用了10幅在42℃时微夹持器的水下工作图像来验证这种光学聚焦方法，结果证明使用光学聚焦方法可以测量出作为驱动器反馈输入的末端弯曲大小，实现对驱动器运动的精确控制。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。