结构连接

As a result of its lower cost and simpler operation method,the mechanical connections promote the quality of concrete structural steel bar connection,accelerate the construction progress and reduce the project cost.

近年来，钢筋机械连接技术发展迅速，国内外均已开发出能充分发挥钢筋母材强度的等强级机械连接接头，施工速度快，成本低，从而为提高混凝土结构钢筋连接工程质量，加快工程进度，降低工程成本创造了条件。

The compensator is made up with compensate pipeline, two flanges fixedly connected with the compensate pipeline and a diversion knot fixedly connected with one of the two flanges and installed in compensate pipeline. Tube wall's structure is as follows: outer layer is fibre enhanced elastomer composite material whose concrete form is fibre cloth enhanced latex mixedly weaved by non-asbestos fibre and steel wire; the second layer is anti corrosive coating wax cloth made by polytetrafluoroethylene; the third layer is thermal protective coating made by glass wool or refractory wool in block shape between two layers of glass fibre clothes; inter layer is dust layer made by glass fibre dilatants voile fabric braided after surface treatment.

本发明的一种多层结构纤维增强弹性体管道补偿器，由补偿管段、与补偿管段两端固定连接的两个法兰、以及一端与两个法兰之一固定连接而安装在补偿管段内的导流套构成，补偿管段的管壁结构为：外层为纤维增强弹性体复合材料，该材料的具体形式是非石棉纤维与钢丝混合编织的纤维布增强橡胶；第二层是由聚四氟乙烯漆布构成的防腐层；第三层是由两层玻璃纤维布之间包覆的块状玻璃棉或陶瓷棉构成的隔热层；内层是经表面处理的玻璃纤维膨体纱编织布构成的防尘层。

In this paper, twenty novel meso-substituted porphyrins of four series were designed and synthesized by increasing the conjugation of porphyrins or introducing substituents with high fluorescence quantum yield into the meso-position of porphine, and then red OLEDs were fabricated by doping porphyrins into a suitable host using ten porphyrins compounds and their electrolumin -escence properties were tested. The main contents and results were generalized as follows:Three meso-tetrakisporphyrins 4a-c and three conjugations of porphine-triarylamine linked by styrene 10a-c were synthesized and characterized by 1H NMR, MS, UV-vis spectra and fluorescence emission spectra.

本文通过增大卟啉分子的共轭结构或将具有高荧光量子产率的基团连接到卟吩环的中位得到了四类共20个新型的卟啉化合物，并选取其中10个化合物制备有机发光器件来考察材料的电致发光性能，具体内容包括以下几个部分：设计合成了3个中位直接被三芳胺取代的卟啉化合物4a-c和3个通过苯乙烯连接的卟吩-三芳胺共轭体10a-c，通过核磁氢谱、质谱、紫外吸收光谱和荧光发射光谱表征了新化合物的结构。

The double-towe-connected structure without enlarged base which is symmetrized in X and Y axises is one simple sort of MTCS. It is formed by tow towers and one or more connecting units which satisfy special functions and architecture demand, such as several spatial corridors.

不设置底盘、双轴对称的双塔楼连体高层建筑是多塔楼连体高层建筑中较为简单的一类，其体型是采用两个塔楼作为主体结构，塔楼间由于功能或造型要求通过一个或多个连接体相互连接而形成的一种复杂结构体系。

The research status of precast concrete structures at home and abroad was indicated. And then a kind of precast concrete frame, whose components are connected by post tensioned prestressing tendons, was introduced in detail. Based on experimental study and theoretical analysis, outline of the design approach of this kind of frame was obtained.

介绍了预制预应力混凝土结构在国内外的研究现状，并着重介绍了一种采用通过后张预应力筋将预制构件连接在一起所形成的柔性连接预制框架结构，在对其进行试验研究和理论分析的基础上给出了设计过程中的一些要点。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Since the lap joint is a key part which greatly influences the fatigue life of aircraft structure,a two-stepped riveted lap joint in an integral panel structure is presented,and finite element analysis is performed for it.

纵向连接接头是影响机身整体壁板结构的安全疲劳寿命的关键部位，因此本文设计了一种适用于机身整体壁板纵向连接的阶梯状两台阶铆接接头结构。

In other words,under the same reaction conditions,the larger the particle size of theprecursor mixed powder,the higher the temperature at which Zn_2TiO_4 starts to appear.There are structure similarity phenomena in ZnO-TiO_2 system,i.e.,the connectionmanner of TiO6 octahedra in the structure of ZnTiO_3 is similar with the one of rutile,andthe connection manner of TiO6 octahedra in the structure of Zn_2TiO_4 and Zn_2Ti_3O_8 issimilar with the one of anatase.

ZnO-TiO_2系统中存在一种类构现象，即：ZnTiO_3结构中的氧八面体的连接方式和金红石TiO_2的相似，而Zn_2TiO_4和Zn_2Ti_3O_8结构中的氧八面体的连接方式则和锐钛矿TiO_2的相似，并由此衍生出一个类构效应理论，即：ZnO和金红石TiO_2的反应产物中不应该出现Zn_2TiO_4和Zn_2Ti_3O_8当然，反应温度应低于ZnTiO_3的起始分解温度。

In studies of interaction among pile, platform that above it and surrounding soil, this paper employ the concept of plate element to simulate the loading character of platform, and employ the concept of solid element to simulate the character of pile and soil, using the finite element method after understanding the geometry properties of pile, soil and the platform. Analyzing the loading properties of the three entities above and their deformation, this paper also employ a special element, Penalty Element. The introduction of penalty element of this kind has served to guarantee the join harmony of displacement between solid element and plate element, thereby effectively resolving the join problem between platforms, pile that support it and surrounding soil.Considering significant property difference between pile material and its surrounding soil, there may be much shear stress on their contact interface produced under vertical load, thus causing displacement or fission. Therefore it is necessary to adopt one special constitutive law of this kind of contact interface, which can reflect its feature of loading and deformation thoroughly. On the basis of the deformation feature and constitutive laws of some contact interface obtained by Zhang Dongji and people concerned through some single-shear experiments on contact interface between structure and soil, this paper employ one pile-soil three-dimensional constant thickness contact interface model to simulate the " slide" taken place on the interface between pile and its surrounding soil. With this kind model, this paper has also created one finite element model, which can be applied to perform finite element calculation and analyze the pile-loading feature, and deduced its element stiffness matrix.

在承台-桩-土共同作用中，考虑桩与承台的几何特性，采用有限元方法，用实体单元模拟桩土，用板单元模拟承台，更具体地分析桩和承台的受力特性和变形，而且还采用罚单元—既实体单元与板单元之间连接必须满足变形协调条件，来有效地解决了承台与桩土的连接问题，且在桩土共同作用中，由于桩体结构的材料性能与周围土层性质相差较大，在竖向荷载下有可能在其接触面上产生较大的剪应力从而导致错动或开裂，因此，为了充分反映桩土接触面上的受力及变形特征，应采用一种桩土接触面特有的本构关系，即利用张冬霁等人关于结构与土体接触面单剪试验所得到的接触面变形特征及其本构关系，引入桩-土三维等厚度接触单元来模拟桩与桩侧土之间的&滑移&，在此基础上建立可用于桩基承载性状分析的有限元计算单元模型，并推导出了各单元的刚度矩阵。

I am sure after a few weeks of adaptation and familiarization I will have no difficulty in functioning on a daily basis in an English speaking society or in participating fully in graduate studies.

在大学的三年的学习生活不仅给予我自身的充实，更重要的是磨练和培养了我较强的自觉能力和严谨，踏实的作风以及不怕困难的精神，对未来充满了信心！

As the first volcanoes erupted, one of the gases that bellowed out was steam.

在第一批火山喷发时，喷出的气体之一就是水蒸汽。