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When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2-4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L-1 after their number counted.

选取2~5代的小鼠胚胎成纤维细胞,待其至对数生长期倒掉或吸掉培养基,加入含10 mg/L丝裂霉素C的细胞全培养液5 mL,置37 ℃,体积分数0.05的CO2饱和湿度温箱中培养二三小时,在6孔板中加入0.1%明胶包被,放置30 min以上,倒掉或吸掉含丝裂霉素C的培养基,磷酸盐缓冲液清洗5遍,尽量除去丝裂霉素C,加入2 mL 0.05%的胰蛋白酶消化细胞,镜下观察,当细胞间出现裂隙,细胞变圆时(约2~4 min),立即加入等量的全培养液终止消化,并用吸管反复吹打,使之成为单细胞悬液,在细胞计数板中央放置专用的盖玻片,用玻璃虹吸管吸取细胞,让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液,至盖玻片下被液体充满为止。

All kinds of these study results, including analyzing the nucleotide composition, the amino acid composition, the nucleotide diversity, the synonymous and the nonsynonymous, the transition and the transvertion, the frequencies of the codon used, the construction (including four stems and four loops) of tRNAleu,the phylogeny,suggest that great distinctness present among the genera and small difference even none among species.

在各种分析中(包括核苷酸基本组成分析;氨基酸基本组成分析;核苷酸多样性分析;非同义替代和同义替代分析;密码子使用频率分析;转换与颠换分析;tRNAleu基因的四臂四环二级结构分析;&翻译启始&和&翻译终止&在进化过程中的变化;系统发育分析),属间的差异相当明显,而种间差异很小甚至是没有任何不同之处。

Private function outFile(fileName:String, data:ByteArray):void { var outFile:File = File.desktopDirectory;// dest folder is desktop outFile = outFile.resolvePath;// name of file to write var outStream:FileStream = new FileStream;// open output file stream in WRITE mode outStream.openoutFile, FileMode.

右括号指示 while 循环、init 方法以及应用程序代码结束,但 outFile 方法除外。执行过程再次返回至 while 循环的开始处并继续处理。zip 文件中其余的字节:提取另一个文件,如果最后一个文件已处理完毕,则终止该。zip 文件处理。outfile 函数以 WRITE 模式在桌面上打开输出文件,同时为其指定 filename 参数提供的名称。

Private function outFile(fileName:String, data:ByteArray):void { var outFile:File = File.desktopDirectory;// dest folder is desktop outFile = outFile.resolvePath;// name of file to write var outStream:FileStream = new FileStream;// open output file stream in WRITE mode outStream.openoutFile, FileMode.

右括号指示 while 循环、init 方法以及应用程序代码结束,不过 outFile 方法除外。执行过程再次返回至 while 循环的开始处并继续处理。zip 文件中其余的字节:提取另一个文件,如果最后一个文件已处理完毕,则终止该。zip 文件处理。outfile 函数将以 WRITE 模式打开桌面上的输出文件,为其指定 filename 参数提供的名称。

Visual carbon stars whose mid-infrared spectra are mainly dominated by photospheric continuum and show large excesses at 60um, represent a transition phase which follows an interruption of mass loss from the oxygen-rich phase and begins a new phase of the mass loss of carbon-rich material. Excesses at 60um in these stars are the emission from the residual of oxygen-rich material.

光学碳星的中红外能量主要来自光球,在60μm处存在超量发射,人们认为其演化阶段处在抛射富氧物质终止和新的富碳拱星壳层形成之间,其60μm超量发射就是富氧物质的残余效应。

On the left,above the promontory, this irregular and jagged cliff descended by a longslope of conglomerated rocks till it mingled with the ground of thesouthern point.

峭壁连绵三英里,右方伸展到一座好象人工凿开的断崖处突然终止了。

An ultra-high strength, weldable, low alloy steel with excellent cryogenic temperature toughness in the base plate and in the heat affected zone when welded, having a tensile strength greater than 830 MPa (120 ksi) and a micro-laminate microstructure comprising austenite film layers and fine-grained martensite/lower bainite laths, is prepared by heating a steel slab comprising iron and specified weight percentages of some or all of the additives carbon, manganese, nickel, nitrogen, copper, chromium, molybdenum, silicon, niobium, vanadium, titanium, aluminum, and boron; reducing the slab to form plate in one or more passes in a temperature range in which austenite recrystallizes; finish rolling the plate in one or more passes in a temperature range below the austenite recrystallization temperature and above the Ar3 transformation temperature; quenching the finish rolled plate to a suitable Quench Stop Temperature; stopping the quenching; and either, for a period of time, holding the plate substantially isothermally at the QST or slow-cooling the plate before air cooling, or simply air cooling the plate to ambient temperature.

在基体板以及焊接时的热影响区处的低温韧性优异的超高强度、可焊接、低合金钢具有高于830MPa(120ksi)的抗拉强度,并且具有包含奥氏体薄膜层和细晶粒的马氏体/下贝氏体板条的显微层状组织,所述钢的制备过程为:加热含有铁以及特定重量百分比的添加元素的钢坯,所述添加元素为碳,锰,镍,氮,铜,铬,钼,硅,铌,钒,钛,铝,以及硼中的一些或全部;在奥氏体可发生再结晶的温度范围内,采用一个或多个道次,将所述钢坯轧制成板材;在低于奥氏体再结晶温度但高于Ar 3 转变点的温度下,采用一个或多个道次对所述板材进行终轧;将所述终轧板材淬火至一适当淬火终止温度;停止所述淬火;或者在空冷前在QST点基本等温保持所述板材一段时间,或者对所述板材进行缓慢冷却,或者只是简单地将所述板材空冷至室温。

The results show that the steel in different solution has better performance of anti-hydrogen induced cracking,there is no cracking inside the steel; micro-cracking on the specimen surface mainly nucleates at pitting corrosion pits and expands along the polygonal ferrite; the expansion mode is mostly transgranular type, and the expansion terminates in acicular ferrite.

研究结果表明:该管线钢在两种溶液中均具有较好的抗氢致开裂性能,试样内部没有发现裂纹;试样表面的微裂纹主要在点蚀坑处形核并沿多边形铁素体扩展,扩展模式多为穿晶型,并终止于针状铁素体。

Units and individuals who employ aliens without permission shall terminate employment of the said aliens and may at the same time be fined from 5,000 to 50,000 yuan, and be ordered to cover all the expenses of repatriating the aliens whom they had employed.

对私自雇用外国人的单位和个人,在终止其雇用行为的同时,可以处5000元以上、50000元以下的罚款,并责令其承担遣送私自雇用的外国人的全部费用。

Units and individuals who employ aliens without permission shall terminate employment or the said aliens and may at the same time be fined from 5000 to 50000 Yuan, and be ordered to cover all the expenses of repatriating aliens whom they had employed.

对私自雇佣外国人的单位和个人,在终止其雇佣行为的同时,可以处5000元以上,50000以下的罚款,并责令其承担遣送私自雇佣的外国人的全部费用。

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推荐网络例句

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克,果实色泽鲜艳,汁多味浓,甜酸适口,离核仁甜,清末时就驰名陕、甘两省,清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20g/hm2;而番茄不敏感。

However, I have an idea.

不过,我有个主意。