细胞分裂

However, it is not known how orthodontic force influences the function of cementoblast and what is the relationship between the function change of cementoblast with root resorption. So we studied the effect of mechanical stimuli on root resoption by examining the expression OPG and RANKL and exploring the related signal transduction pathway in cementoblast. Methods: Cementoblasts OCCM30 were cultured in DMEM with 10% FBS for 48 hours and starved in DMEM with no FBS for 24 hours, then subjected to mechanical strain by four-point bending system with tension and compression stress at 2000μstrain at 0.5Hz frequency. The flow cytometry was used to examine the cell cycle and proliferation activity after 3h, 6h, 12h, 24h loading respectively. OPG and RANKL mRNA were analysed with real time quantitive RT-PCR after the same loading period as FCM.

本课题利用四点弯曲力学加载系统，采用流式细胞术、实时荧光定量PCR、Western免疫印迹等方法，研究体外培养的成牙骨质细胞株OCCM-30在不同力学环境中增殖活性的变化，以及其表达骨保护素和破骨细胞分化因子的mRNA的变化，探讨应力刺激对成牙骨质细胞表达破骨细胞分化调控蛋白的影响，并通过对应力刺激下成牙骨质细胞分裂原活化蛋白激酶家族中的ERK1/2、P38MAPK活性的变化及其与OPG、RANKL基因表达变化关系的研究，对力学刺激调节破骨细胞分化调控蛋白的相关上游信号转导通路进行初步探讨。

Is highly debated. Microscopic studies using DMACA staining revealed flavanols in shoot tip cells of Tsuga Canadensis during the early anaphase and in prophase nuclei.

用DMACA染色法的显微研究表明在在加拿大铁杉茎尖细胞分裂后期的早些阶段及细胞分裂早前期的细胞核中都有黄烷醇存在。

Second Municipal People's Hospital of Guangzhou, Guangzhou 510150 Objective To study the technique and diagnostic value of fluorescence insitu hybridization in chromosome abnormality for prenatal diagnosis. Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium.

对34例孕16～23周有产前诊断指征者经腹部抽取羊水，用Chang培养液传代培养，常规制备羊水细胞分裂中期染色体，应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针，与培养的羊水细胞分裂中期染色体DNA进行原位杂交，杂交后用荧光显微镜观察玻片并摄像。

Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium. The metaphase chromosomes were hybridized insitu with the human centromere probes,α-satellites DNA probes of X, Y,13, 21, 18 chromosomes and all primer chromosome probes. These probes were conjugated by Biotin and Dioxin then. The treated slides were examined and taken photos under the fluoromicroscope.

对34例孕16～23周有产前诊断指征者经腹部抽取羊水，用Chang培养液传代培养，常规制备羊水细胞分裂中期染色体，应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针，与培养的羊水细胞分裂中期染色体DNA进行原位杂交，杂交后用荧光显微镜观察玻片并摄像。

Cytokinin KT and 6-BA were evaluated for their function in callus induction and plant regeneration. The results showed that cytokinins improved the regeneration capacity of maize and 6-BA was more effective than KT.

研究了细胞分裂素在玉米愈伤组织诱导和植株再生中的作用，结果表明低浓度(0.2mg/L)的细胞分裂素能促进玉米幼胚诱导的愈伤组织再生，6-BA的效果比KT更好。

Cytokinin KT and 6-BA were evaluated for their function in callus induction and plant regeneration. The results showed that cytokinins improved the regeneration capacity of maize and 6-BA was more effective than KT.

黄璐研究了细胞分裂素在玉米愈伤组织诱导和植株再生中的作用，结果表明低浓度(0.2mg/L)的细胞分裂素能促进玉米幼胚诱导的愈伤组织再生，6-BA的效果比KT更好。

Strong root activity in hybrid combinations is relatedto slower decrement in total amount of cytokinins.

杂交组合根系伤流液中细胞分裂素数量高于恢复系，杂交组合根系活力强，与细胞分裂素总量下降缓慢有关。

Cultured cells lose the ability of DNA synthesis, mitosis, and finally the ability of cell proliferation after they have undergone a finite number of population doublings in vitro, though the cells still maintain the basic metabolic process.

体外连续培养的细胞在有限次数的细胞分裂后，丧失合成DNA及分裂的能力，最后导致增殖能力的丧失，但基本代谢过程仍能维持，这种现象称为复制衰老。

Base on MS medium, four kinds of auxin IAA,IBA,NAA,2,4 D and three kinds of cytokinin BA,KT,ZT were arranged in monofactoral experiments. NAA and BA were selected to be the best regulators for regeneration of folioles and raches of the compound leaf.

以MS为基本培养基，对IAA、IBA、NAA、2 ，4 D 4种生长素，及BA、KT、ZT 3种细胞分裂素进行单因子试验，对比并筛选出对刺槐小叶和叶轴再生最好的生长素和细胞分裂素为NAA、BA。

Ontinues each kind of game character outsourcing manufacture, the members have the rich manufacture experience, once had manufactured the many patterns large-scale game in various macrocosms famous game company, involves to each kind of platform for example: Splinter Cell 4-Double Agent (UBISOFT The X-BOX360 platform / Splinter Cell 4-Double Agent / Brothers In Arms2 (UBISOFT The PS2 platform)/ Brothers In Arms / Ghost Recon 2 / RYMAN / END WAR (UBISOFT X-BOX360)/?M2 / Iron Phoenix (IS The X-BOX platform)/ Zill O'll infinite (KOEI The PS2 platform) and so on.

承接各类游戏角色外包制作，其成员都拥有丰富的制作经验，曾在各大世界著名游戏公司制作过多款大型游戏，其中涉及到各种平台（如：《细胞分裂4-双重间谍》（UBISOFT X-BOX360平台）、《细胞分裂4-双重间谍》（UBISOFT PC平台）、《兄弟连》（UBISOFT PS2平台）、《兄弟连》（UBISOFT PSP平台）、《幽灵行动》、《雷曼》、《最终之战》（UBISOFT X-BOX360）、《神甲骑兵》、《流星蝴蝶剑2》、《龙士传说》（日本光荣 PS2平台）等。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。