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Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因,用PCR、测序鉴定缺失菌株,通过动物实验观察clpE基因缺失株毒力改变情况,同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力,最后用实时荧光定量PCR分析自溶素(major autolysin A,lytA)、表面黏附素A(pneumococcal surface adhesion A,psaA)、溶血素(pneumolysin,ply)、肺炎球菌表面蛋白A(pneumococcal surface protein A, pspA和神经氨酸酶(neuraminidase, nanA)的表达。

A highly flammable, colorless or yellowish syrupy solution of pyroxylin, ether, and alcohol, used as an adhesive to close small wounds and hold surgical dressings, in topical medications, and for making photographic plates.

火棉胶一种极易燃的、无色或黄色糖浆状火棉、乙醚和酒精混合溶液,用于局部药物,治疗中粘涂愈治小的创伤和及附外科包扎,并可制作底片

In an accompanying editorial, Loren Laine, from the University of Southern California School of Medicine in Los Angeles, notes the marked variability between studies of adherent clots.

在一篇附论中,洛杉矶南加利福尼亚大学医学院的Loren Laine指出,粘著性血凝块的研究中存在著明显的可变性。

With the microelectromechanical technique, we developed the taste cell chip based on LAPS, the surface of which had already been soaked in the phosphate-buffered saline with 100μg/ml poly-L-ornithine and 8μg/ml laminin to enhance the attachment of cell on it.

采用微机械加工技术,我们制备了LAPS味觉细胞芯片,并用多聚鸟氨酸和层粘素混合的磷酸缓冲液处理以增强细胞在芯片表面的黏附性。

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What are your goals and strategies for growth?

你的成长目标和策略是什么?

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3:1 你要写信给撒狄教会的使者,说,那有神的七灵和七星的,说,我知道你的行为,按名你是活的,其实是死的。

"It is a testament to making sure unemployment compensation is available, making sure we are looking out for people who have lost their jobs," she said.

"这是个实证,证明我们必须确保人们都可以得到失业补偿,确保那些失去工作的人们得到照顾。"