粘质

Considering the gel layer and the endolymph of otolith organs as visco-elastic solid and fluid respectively, the Grant′s elastic model was modified.

将耳石器官的胶质层和内淋巴液分别作为粘弹性固体和流体处理，修改了Grant等人所提出的模型，通过频率分析，给出了耳石器官物性参数以及粘弹特性对于系统的不同控制作用。

Objective To determine the expression of CD44 in the liver tissue.Method 26 cases of liver cancer,16 cases of hepatocirrhosis and 5 cases of normal liver tissue were studied by HE stain and immunohistochemistry.

CD44是一种粘附分子，能介导细胞与细胞以及细胞与基质间的粘附，近年来的研究发现它与人体肿瘤的发生、发展及转移有着密切的关系，对肿瘤病人的预后判断具有重要的意义。

Cluster of differentiation 44(CD44) is a principal cell surface receptor for hyaluronate, a major component of extracellular matrices.

人类白细胞分化抗原作为一种粘附分子，主要与细胞外透明质酸结合，参与关节软骨基质中透明质酸的代谢，因而它与各种骨关节疾病包括大骨节病关系密切。

The result indicated that suitable thickness of paddy soil is about 15cm,and for dry arable land it should not be lower than 20cm;The alluvial rivers parent material is the best one to influent the output,the rice fields is the worst with the parent material of slate ground,while the dry and plough land is the worst for its purple sand shale parent material;The paddy rice is best with the acid soil,and the dry crop is the best with the neutral soil;The obstacles horizon exist in 35.2%of the rice fields in research region,and many for middle or top digit obstacles.

结果表明，水稻土的适宜耕层厚度为15 cm左右，旱耕地适宜耕层厚度应不低于20cm；成土母质对产量的影响以河湖沉积物母质最好，稻田以板页岩母质最差，旱耕地以紫色砂页岩母质最差；水稻以酸性土最好，旱作以中性土最好；研究区稻田有35.2%的存在障碍层，且多为中、高位障碍，是影响水稻产量整体提高的主要因素；水稻和旱作都以壤土为最好，其中，稻作怕砂，旱作怕粘；水稻土适宜的容重为1.00—1.29 g/cm~3。

Studies on stability indicated that PHGF proliposomes should be stored at low temperature and against light, which ensured unchange of their redispersity, content of drug, encapsulation efficiency, acid value and peroxide value. Liposomes were multilamellar vesicle structure under transmission electron microscopy. Average particle size was 0.65 micrometer, ranging from 0.09 micrometer to 1.21 micrometer. Cryoscopic value was 0.64 C determined by cryoscopic method, which was a little higher than that of isotonic solution.

以透射电镜观测PHGF前体脂质体再分散后的形态为囊泡状；激光散射法测得平均粒径为0.65μm，且粒度分布在0.09μm～1.21μm之间；该脂质体荷负电，ζ电位为-44.23mV；冰点降低法测得冰点降低度数为0.64℃，略高于等渗溶液冰点降低度数；测得粘度为1.83mPa·s，达到生理要求；此外，对PHGF脂质体的刺激性、溶血性、热原、异常毒性和过敏沈阳药科大学硕士学位论文拘要性检查表明均符合静脉注射给药的标准。

Several points can be concluded for the area from this paper study:(1) Hydrocarbon mainly occurs in the lower interval of bioclastic limestone and dolomitic limestone(the special lithologic interval ) in Es_1 Member,in which solution pores well developed;(2) Traps are mainly anticlines controlled by f.

本文研究表明，大中旺地区油气主要分布在沙一段下部的生物碎屑灰岩和云质灰岩段，储层溶蚀孔隙发育；圈闭主要为受断层控制的断背斜构造；原油具有高密度、高粘度、中低凝固点、高初馏点、高含硫量和高胶质沥青质等特点，属于未成熟—低成熟油；油源为本地咸水环境下形成的沙一段暗色泥岩。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The soil and bark samples collected from Yunnan, Hebei and Shanxi provinces of China, and also from Vietnam, were used as the materials in this experiment. And dung pellets of rabbits or goats that had been autoclaved can be used to induce many kinds of fruiting bodies of the Myxobacteria in different colors, sizes, and shapes. We isolated and purified Myxobacteria strains with the improved method which was found by our laboratory.

本实验以采集自越南以及我国的云南、河北、陕西各地的土样和各种树皮、腐木作为实验材料，利用兔、羊粪球和滤纸作为基质诱导出多种颜色丰富、形态多样的粘细菌子实体，采用本实验室摸索出的较为有效的纯化粘细菌的方法，在实验器材上用24孔细胞培养板代替传统的平皿，有效地缩短了粘细菌的纯化周期，提高了分离纯化的效率。

Mo-Ni-P catalyst is a highly effective desulfurer and denitrider and in addition, has a favourable degradation effect on the dehydrogenated products and makes the viscosity og the reformed oily liquid less susceptihle to the effect of the existence of a N_2 atmosphere.

Mo—Ni—P催化剂脱S，脱N效果好，加氢改质产品轻质化效果佳，改质液化油在N_2气氛下粘度变化较小。

To master fluid mosaic model of cell membrane;peripheral proteins and integral proteins;the type of extracellular matrix;Collagen;glycosaminoglycan and proteoglycan;elastin; Occliding junctions;anchoring junctions;desmosome hemidesmosome、the structure and function of adhesion belt and focal adhesion;cell coat;cadherins;selectin;Ig-superfamily; integrins

掌握生物膜的流动镶嵌模型；膜周边蛋白和膜内在蛋白；细胞外基质的类型；胶原纤维；糖胺聚糖和蛋白聚糖；弹性蛋白；封闭连接；锚定连接；桥粒与半桥粒、粘着带与粘着斑的结构与功能；细胞外被；钙粘素；选择素；免疫球蛋白超家族；整联蛋白

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。