移植

By the examination of MRI, histopathology, and the caculation of survival rate and percentage of patency, the viability of graft status of autograft tracheas wrapped in one-side sternomastiod muscule flap and two-side sternohyoid muscle flap based on different pedicle, effects of neck muscle associated with bFGF on length of autografts, and limit of warm ischemia time on tracheal allograft before cryopreservation were evaluated in rabbits.

肿瘤、先天性疾病、炎症或其他原因引起的气管狭窄，切除病变气管行气管端端吻合术是最理想的方法。而长段气管切除术后，则需要行气管重建。20世纪以来随着免疫学的发展，肾脏、心脏等脏器移植已在临床应用成为可能，人们注意到气管移植的重要性。因此，气管移植成为长段气管缺损重建的方向，其面临的主要问题是移植气管的再血管化、免疫抑制、供体的来源与保存等。

It is suggested that Ginaton may improve the microcirculation of the xenografts and lighten the HAR. The PVBF after FK506 pretreatment were 0.466±0.049, 0.399±0.037, 0.358± 0.040, 0.317±0.039 and 0.258±0.033kHz, the survival time was 215. 9±16. 0min, significantly higher and longer than those of the nontreatment recipients.

发现移植前30分钟应用金纳多移植后0、15、30、45和60分钟多普勒超声测得的移植肝PVBF波幅分别为0.406±0.020、0.357±0.025、0.308±0.013、0.249±0.019和0.205±0.014kHz，与未用药组相比其降低的程度有明显降低，说明金纳多具有改善豚鼠至大鼠移植肝的微循环，具有减轻排斥反应的作用。

At present, both liver and heart transplantations have been becoming the only available means for treating terminal liver and heart parenchymatous diseases.

无论是肝脏移植还是心脏移植，目前都已成为治疗中末期肝病和心脏器质性病变的唯一有效手段，至今临床肝脏或心脏移植全球累计总例数都已逾6万例，并且移植疗效良好。

Under hypoxic conditions,prolyl hydroxylase activity decreases because they require oxygen as a co-factor and the HIF-1αprotein is stabilized.HIF-1αstabilization is followed by activation of the transcription of numerous target genes such as vascular endothelial growth factor,erythropoietin,and glucose transporter 1 (Glut-1) that are involved in angiogenesis,erythropoiesis,energy metabolism and cell survival.

但是，MSCs改善心功能的能力有限，大量的证据表明MSC移植对心功能的改善是轻中度的，大部分研究结果显示MSCs移植对心功能的改善只有5-6%左右，甚至有些临床试验没有观察到心功能的改善，这远远达不到我们的预期，其中一个很重要的原因是绝大多数的细胞移植到体内后在很短的时间内发生死亡，研究报道90%的MSCs在移植后的1天内死亡。

Egr-1 mRNA and Egr-1 protein hadn't been found in the normal vein. The expressions of Egr-1 mRNA and Egr-1 protein had biphasic changes. By reverse transcription-PCR and in situ hybridization, we found that the level of Egr-1 mRNA rose at 1 hour after graft, the expression of Egr-1 mRNA was (35±7)%. Decline at hour 6, 24 and day 3, the positive rates of Egr-1 mRNA were (8±2)%,(8±6)% and (8±4)% respectively. Reincrease at day 7, a peak at day 28, the positive rate of Egr-1 mRNA was (45±6)%(compared with other phase, P＜0.01). At day 42, the expression of Egr-1 mRNA declined again. Immunohistochemical staining and Western blot revealed Egr-1 protein had expressed at hour 2 early phase, the expression of Egr-1 protein was (30±5)%, and until to hour 6. The level of Egr-1 protein was decrease at hour 24 and day 3, the positive rates were (7±3)% and (7±8)% respectively.

结果 自体静脉移植后，Egr-1 mRNA和Egr-1蛋白的表达呈双相变化，即移植后1 h， Egr-1 mRNA表达迅速升高，阳性率为（35±7）%，6 h、24 h及3 d时下降到较低水平，阳性率分别为（8±2）%、（8±6）%和（8±4）％，7 d时又再升高，28 d时达高峰，阳性率为（45±6）%，此与其余各时点比较差异均有统计学意义（P＜0.01），42 d时，Egr-1 mRNA的表达再次下降；移植早期（2 h）即有Egr-1蛋白的表达，阳性率为（30±5）%，并持续至6 h，24 h～3 d表达下降到较低水平，阳性率分别为（7±3）%和（7±8）%，7 d时又再升高，至移植后28 d，Egr-1蛋白的表达阳性率达到高峰，为（40±9）%，此与其余各时点比较差异有统计学意义（P＜0.01）。

Corneal allograft is the most effective treatment for corneal blindness,but corneal graft rejective reaction is the main cause of corneal allograft. Corneal neovascularization is one of the most causes of corneal blindness and the high-risk factor of corneal graft rejective reaction.

研究背景同种异体角膜移植是治疗角膜盲最为有效的方法，而角膜移植排斥反应仍然是移植失败的主要原因；角膜新生血管是角膜盲的主要原因，亦是角膜移植排斥反应的高危因素。

The indications were: acute liver failure in one (4%); chronic liver failure in 25 (96%)-10 metabolic, six biliary atresia, five polycystic/liver fibrosis, four other, and one retransplant. Nine (35%) received partial graft; 5 (19%) multivisceral grafts (liver-kidney, liver-bowel) and 12 (46%), conventional OLT.

适应症：急性肝功能衰竭1例（ 4 ％）；慢性肝功能衰竭25例（ 96 ％），其中10例代谢病，6例胆道闭锁，6例多囊肝/肝纤维化，其他4例中一个为再次移植。9个（ 35 ％）接受部分肝移植； 5个（19 ％）接受多脏器移植和12个（46 ％）接受常规肝移植。

Results The level of antibodies against monocytes (34.94%) were higher than that of the antibodies against lymphocytes (26.67%) in pretransplant patients. The level of antibodies in first time transplant patients (32.5%) was lower than that of antibodies in retransplant patients (59.7%). The patients with intensity of antibodies against monocytes PRA more than ≥50%only accounted for 1.87%, 10%MPRA50%for 17.62%and MPRA10%15.44%,respectively.Conclusion Before renal transplantation, the detection of MPRA level could predict the occurrence of rejection in renal transplantation.

结果 肾移植术前患者的抗单核细胞抗体水平较抗淋巴细胞抗体水平高，其百分比分别为34.94%和26.67%；而从患者初次和再次肾移植前的比较中可见，初次比再次产生的抗单核细胞抗体水平要低，其百分比分别为32.05%和59.7%；对于抗单核细胞抗体群体反应性抗体的强度，MPRA≥50%的只占1.87%，而50%10%以及MPRA结论在肾移植术前检测患者MPRA水平，对肾移植后的排斥反应能起到预测目的。

MSCs were transplanted to infracted area of brain stereotactically,for comparsion with sham operation transplantation group and CI control group...

结果：移植入脑的MSCs在正常脑组织与梗死区能存活至少4周以上，并发生迁移，至第3周移植治疗组迁移范围为(3.626±1.831)mm2，明显比假手术移植组(1.534±0.475)mm2大，差异有显著性意义(F=5.7863，P.05)；接受MSCs移植后大鼠改良神经功能缺损量表得分低于另两组。

Methods Eighty Class Ⅱ isogeneic F344 rats, weighing 250-350 g, were randomly divided into 2 groups(n=40). The control group adopted the Strome model. The allograft used for the experimental group preserved ascending pharyngeal artery, which formed a complex allograft together with tongue base, larynx and pharyngolarynx. End-to-end anastomosis was performed between both allograft common carotid arteries and anterior neck veins. The patency rate and survival rate of the allograft of the 2 groups were compared.

80只Ⅱ级近交系F344大白鼠，体质量250~350 g，随机分为2组，每组40只，共20对对照组采用Strome喉移植模型，实验组在切取供体喉时保留咽升动脉，将舌根、喉、喉咽作为复合移植体，供体大鼠双侧颈蓉动脉分别与受体大鼠颈总动脉、颈前静脉进行端端吻合重建移植喉血液循环，比较两组术后动静脉通畅率及移植喉存活率。

He knows that he's one of the mostdominant players in the NBA right now.

我知道他现在是联盟里最有统治力的球员之一了。

Using analysis method of BCG , the dissertation analyzed the management of voluntary service organizations .

利用BCG分析方法对我国的志愿服务组织管理进行了分析。