科鱼

Tropical fish: Including characin, cyprinidae, medaka, cichlid, gourami , biota or loach and groupers and so on.

热带鱼：包括脂鲤科、鲤科、鳉鱼科、慈鲷科、攀鲈科、鳅科、鲶科等。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

Any of various fishes of the family Cyclopteridae,especially Cyclopterus lumpus of North Atlantic waters,having pelvic fin s united to form a suction disk and a body bearing prominent tubercles.

圆鳍鱼圆鳍鱼科的几种鱼，尤其是北大西洋圆鳍鱼，有结合成吸盘的腹鳍，全身呈永久结节状

Any of several widely distributed, chiefly marine fishes that are primarily of the families Blenniidae and Clinidae and have small, elongated, often scaleless bodies.

鳚鱼一种分布广，主要鳚科和热鳚科的海水鱼，有小而细长，常为无鳞的身体

Any of various fishes of the family Cyclopteridae, especially Cyclopterus lumpus of North Atlantic waters, having pelvic fins united to form a suction disk and a body bearing prominent tubercles.

圆鳍鱼圆鳍鱼科的几种鱼，尤其是北大西洋圆鳍鱼，有结合成吸盘的腹鳍，全身呈永久结节状

Using "neighbor-joining" of Mega 2.1 software reconstructed the molecular Phylogenetic Tree, the results indicated Labeoninae fishes might not be monophyletic population, C.molitorella and E.bicolor of Labeoninae assembled together with Cyprinus carpio and Carassius auratus firstly, and formed sister group, then assembled together with C.mrigala and L.rohita , which suggested that C.molitorella was genetically more relative to Cyprinidae than Labeoninae.

用Mega 2.1软件的NJ法构建分子系统树，结果表明：野鲮亚科鱼类没有形成单系类群，野鲮亚科的鲮、角鱼与鲤亚科的鲤、鲫形成姐妹群，然后再与野鲮亚科的麦鲮和露斯塔野鲮聚在一起，说明鲮与鲤亚科鱼类具有较近的亲缘关系。

Any of various marine food fishes of the family Hexagrammidae, which frequent rocky coastal areas of the northern Pacific.

六线鱼，长蛇齿鱼六线鱼科的以海洋生物为食的任一种鱼，常见于北太平洋岩石海岸海区

The study results showed that Pomacentridae and Labridae are the two most dominant families and the Pomacentrus coelestis is the most abundant species during day time. Species composition of coral reef fish assemblages at the four stations changed more or less each year. The Sorensen similarity values of the four stations fluctuated between 60-75%, fluctuation is mainly due to the changes in rare species.

其结果目前已知雀鲷科与隆头鱼科为此海域的优势鱼科，白天优势种则为变色雀鲷，4个测站都观察到每年鱼种的组成均略有改变，四测站Sorensen相似度约在60%~75%之间，差异主要来自稀有种。

The results show that stocks of Trichiuridae,Decapterus maruadsi,Trachurus japonicus,Nemipterusand Priacanthidaeare 5.04×10~(5),1.63×10~(5),1.04×10~(5),9.8×10~(4) and 9.2×10~(4) t,respectively,in the north waters of South China Sea.

根据调查资料，运用渔业资源声学评估方法对该海域的带鱼科、蓝圆鲹、竹荚鱼、金线鱼科以及大眼鲷科等5类23种经济鱼类进行了评估与分析。

Large family of small soft-finned fishes; killifishes; flagfishes; swordtails; guppies.

小型软鳍鱼类的一个大科；淡水鳉科小鱼；花尾汤鲤；剑尾蝽；古比鱼。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。