磷酸氢二钠

EDTA, sodium dihydrogen phosphate and ammonium metavanadate also presented an inhibitory effect on the activity of the enzyme.

EDTA、磷酸氢二钠和偏钒酸铵对该酶活力也有抑制作用。

The invention discloses a preparing method of bacillus alcaligenes strain Alcaligenes sp., CCTCC No.M206121 of degraded o-nitrophenol, which comprises the following steps: allocating inorganic salt culture medium with dibasic sodium phosphate, monobasic potassium phosphate, manganese sulfate, copperas, addex-magnesium, calcii chloridum, blue copperas, white copperas and hydrogen dioxide solution; adjusting pH value; sterilizing under high temperature; enriching bacterial; sampling from sludge; allocating suspending liquid with soil sample and inorganic salt; seeding into ortho-nitrophenol fresh organic salt culture medium with finite volume seeding quantity; keeping the temperature; culturing on shaker; getting enriched culture; separating and purifying enriched culture in LB solid culture medium.

本发明公开了一种降解邻硝基酚的产碱杆菌菌株及制备方法，产碱杆菌为Alcaligenes sp。，CCTCC No.M206121。其制备步骤是：首先是配制无机盐培养基，成分为磷酸氢二钠、磷酸二氢钾、硫酸锰、硫酸亚铁、硫酸镁、氯化钙、硫酸铜、硫酸锌和双蒸水，调pH值高压灭菌；其次是菌种的富集，从污泥中取样，将土样与无机盐培养基配制成悬浮液，将悬浮液以一定体积接种量接种到含邻硝基酚的新鲜无机盐培养基中，恒温，摇床培养，获富集培养物；第三是将富集培养物在LB固体培养基中分离和纯化。

Methods: The content of 7-ACA raw material was determined on C18chromatographic column by using disodium hydrogen phosphate reagent.

用磷酸氢二钠试剂，在C18色谱柱上测定7-氨基头孢菌烷酸原料的含量。

Methods: The analytical column was SHIMADZU VP-ODS C18 column(4.6 mm×150 mm, 5 μm) with a mobile phase consisted of 0.05 mol/L disodium hydrogen phosphate solution(85% phosphoric acid solution with pH adjustment to 2.5)-acetonitrile-methanol(42.5∶47.5∶10) at the flow rate of 1.0 ml/min, the detection wavelength was set at 254 nm.

色谱柱为SHIMADZU VP-ODS C18柱(4.6 mm×150 mm，5 μm)，流动相为0.05 mol/L磷酸氢二钠溶液(用85%磷酸溶液调节pH值至2.5)-乙腈-甲醇(42.5∶47.5∶10)，流速为1.0 ml/min，检测波长为254 nm。

Another method was studied for in-situ synthesizing sodium alginate grafted sodium acrylate in disodium hydrogen phosphate dodecahydrates' melt.

本论文提出了另一种方法，是在十二水磷酸氢二钠熔体中，原位合成海藻酸钠接枝丙烯酸钠共聚物作为增稠剂。

Methods: The analytical column was SHIMADZU VP-ODS C18 column(4.6 mm×150 mm 5 μm) with a mobile phase consisted of 0.05 mol/L disodium hydrogen phosphate solution(85% phosphoric acid solution with pH adjustment to 2.5)-acetonitrile-methanol(42.5∶47.5∶10) at the flow rate of 1.0 ml/min the detection wavelength was set at 254 nm.

目的：建立丙酸氯倍他索尿素乳膏中丙酸氯倍他索含量的HPLC测定方法。方法：色谱柱为SHIMADZU VP-ODS C18柱(4.6 mm×150 mm5 μm)流动相为0.05 mol/L磷酸氢二钠溶液(用85%磷酸溶液调节pH值至2.5)-乙腈-甲醇(42.5∶47.5∶10)流速为1.0 ml/min 检测波长为254 nm。

Then,in the bovine serum albumin buffer solution containing disodium hydrogen phosphate and citric acid,ferrite/polyacrylamide was added with crosslinker glutaraldehyde and then be enwrapped by BSA.

首先在反相微乳液体系中制备出Fe3O4/PAM磁性微球；然后在牛白蛋白的磷酸氢二钠-柠檬酸缓冲溶液中，以戊二醛为交联剂，加入Fe3O4/PAM微球，使蛋白质特异性吸附在微球上。

Disodium hydrogen phosphate dodecahydrates possesses the largest mass heat storage capacity among the low temperature phase change materials, which are classified as inorganic, organic and then: composites, yet it experiences phase segregation and super cooling when melting and hinders its application in latent heat storage system.

低温相变材料可分为无机、有机物及其复合物。十二水磷酸氢二钠(Na_2HPO_4·12H_2O)是其中质量储热密度最大的一种，但由于熔化时发生相分离，而妨碍了它的实际应用。

An ODS column was selected as the separation column at 25 ℃, and pH 4.50, 0.02 mol/L of trisodium citrate-0.05 mol/L sodium phosphate dibasic to methanol (95:5, volume ratio) without ion-pair at a flow rate of 1.0 mL/min.

该实验采用ODS柱，流动相为V(0.02 mol/L柠檬酸三钠-0.05 mol/L磷酸氢二钠)：V=95：5的溶液，用电化学检测器检测。

The mobile phase was 30% volume of acetonitrile, 70% volume of phosphate buffer salt(sodium hydrogen phosphate 1.12 g, sodium dihydrogen phosphate 0.18 g, add water to 1000 mL, adjusted to pH 7.6); the detection wavelength was set at 288 nm.

建立HPLC法，色谱柱：Diamonsil C18(5μm， 4.6 mm×250 mm)，流动相：乙腈－磷酸盐缓冲液（取磷酸氢二钠1.12 g，磷酸二氢钠0.18 g，加水溶解并稀释至1 000 mL，调pH值至7.6）（体积比为30：70），检测波长：288 nm。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。