病毒体

Systemic therapy: Cyperus, 12 grams each Equisetum煎服, dregs outside partial rub, for the 7-10 day course of treatment can disappear off疣体. 3, the virus will not invasive cardiopulmonary 4, that can cure 5, died may also disappear naturally.

全身疗法：香附、木贼各12克煎服，药渣外擦局部，疗程为7-10日，可使疣体脱落消失。3、病毒不会侵入心肺4、可以根治5、不治也可自然消失。

At each penton, a hole (φ=20〓) can be seen connecting the inside and the outside of the capsid, and there is a hole (φ=12〓) at each hexton and the center of 2-fold axis, so there are 132 holes in total at the surface of CSBV.

应用冷冻电镜技术和计算机三维结构重构的方法，我们测定了CSBV核衣壳的三维结构，分辩率为25 〓，病毒的核衣壳是按T=1（P=3）的对称结构排列二十面体，病毒表面比较光滑，有12个五邻体、60个六邻体的结构，每个五邻体的中央有孔洞，六邻体的中央也有孔洞，另外在2次轴的位置上也有孔洞，这样病毒共有132个孔洞，五邻体的孔洞的直径为20 〓，而六邻体和二次轴的孔洞的直径月为12 〓。

So that precipitate any residual of virus.

所以可以结合所有残留的病毒体。

EXE file in the Windows system directory, writes its "pure" code to there and runs this file.

EXE的文件，写入病毒体本身，并运行之，这个病毒的拆离体

Recombinant plasmid pSVH 7 DNA of avian influenza virus H7 subtype heamagglutinin gene was encapsulated with DC-chol/DOPE liposomes and PC/chol/SA liposomes separately. Two-week old SPF chickens were intramuscularly inoculated with 50 μ g/0.2ml of the liposome entrapped PSVH 7 DNA. Four-weeks later, each chicken was challenged with 0.1ml 〓 AIV . One week after the challenge, the secretion of the cloacas was collected and transfected to chicken embryos to isolate the virus. The virus was isolated from 6/6 of the control group, 1/6 of the naked DNA group, 1/6 of the PC/chol/SA entrapped DNA group and 0/6 of the DC-chol/DOPE liposome entrapped group. The HI antibody titers (log2) of the four groups were 6. 83±0.98, 7. 0±1. 26, 7. 83±1. 17 and 8. 00±0.89 respectively 1-week after challenge, and 8. 5±0.55, 8. 17±0.82, 8. 68±0.45 and 9. 33±0.54 respectively 2-week after challenge. The results showed that inoculation of liposome entrapped DNA significantly enhanced resistance to virosis in animals.

将含禽流感病毒H7亚型血凝素基因的重组质粒pSVH7用DC-chol阳离子脂质体和胆固醇／卵磷脂／十八胺脂质体包裹，免疫2周龄SPF鸡，4周后用同型禽流感病毒进行人工感染，1周后采集泄殖腔分泌物分离病毒，结果未免疫组6／6分离到病毒，裸质粒DNA免疫组1／6分离到病毒胆固醇／卵磷脂／十八胺脂质体包裹DNA免疫组1／6分离到病毒，DC-chol脂质体DNA免疫组没有分离到病毒（0／6）：人工感染后1周各组的HI抗体（Log2）分别为6.38±0.98，7.00±1.26，7.83±1.17，8.00±0.89，2周后为8.50±0.55，8.67±0.82，8.68±0.45，9.33±0.52，脂质体包裹组在同期均高于未免疫组和裸DNA免疫组，表明脂质体包裹质粒DNA免疫动物后，能增加动物对病毒感染的抵抗力和反应能力。

In the rice leaves infected by RDV Yunnan isolate, the distribution of cells full of virus particles formed a belt which was a center of infection and multiplication of virus, around the cells fall of virus particles were some cells with the different extent of lesion as follows: disappearance of membrane system of chloroplasts, there were virus particles in some chloroplasts, and some sine or assembled virions in the mitochodria or cytoplasma but not in nuclei ,and intact subcellular structure in the cell far away from the infected center; In the rice leaves cell infected by Zhejiang isolate, some cells were full of virions, but the distribution of cells didn't form the belt, other cytopathological characters were similar to the cells infected by Yunnan isolate.

RDV云南分离株浸染的水稻叶片中，充满病毒粒体的细胞呈条带状分布，呈现出明显的侵染和增殖中心，在充满病毒粒体的细胞周围，相邻细胞有不同程度的病变，叶绿体膜系统消失，部分叶绿体中含有病毒颖粒，线粒体残体及细胞质中有分散或聚集的病毒粒体，细胞核中未见病毒粒体，离侵染中心较远的细胞中亚细胞结构较完整；RDV浙江分离株侵染的水稻叶片中，部分细胞充满病毒粒体，无明显的条带状分布，其他细胞病理特征与RDV云南分离株侵染的水稻叶片相似。

A pair of primers producing 614bp amplification fragment were designed based on the sequence of the C\|polyhedrin gene of Bombyx mori CPV,and the expected amplification products were obatined when genomic dsRNAs isolated from purified BmCPV,DsCPV and Lymantria dispar CPV were used as templates.Genomic dsRNAs isolated from Heliothis armigera CPV and DNAs from Lymantria dispar nuclear polyhedrosis virus and DNAs from midgut tissue of healthy Dendrolimus spectabilis larva did not yield any amplification products.The detection limit of purified DsCPV genomic dsRNAs was 1.0pg.

依据家蚕质型多角体病毒质型多角体蛋白的核苷酸序列设计一对引物，从纯化的DsCPV\，BmCPV和舞毒蛾质型多角体病毒的基因组dsRNA可成功地扩增出长614bp的目的片段，从提取的健康松毛虫幼虫肠组织的DNA、舞毒蛾核型多角体病毒基因组核酸、以及棉铃虫质型多角体病毒基因组核酸，未能扩增出目的片段。

Viral vector mainly contain adenovirus, retrovirus,adeno-associated virus and so on, but it has potential danger of safety; it isrepeled by immune system when it is injected to organism for a greatimmunogenicity. An injection with adenovirus vector with highconcentration, it leads to serious inflammatory reaction of liver. Viralvector such as liposome and polycation are commonly used lately. But,liposome and polycation have low specificness and targetness of genetransfer tissue, have lower transfection efficiency and short period ofgene expression, for they can be phagocytized by endothelial system.

许多的载体，病毒载体和非病毒载体以前已广泛应用，病毒载体主要包括腺病毒，逆转录病毒，腺相关病毒等；但病毒载体在安全性方面存在潜在的危险；免疫原性比较强，注射到机体后很快会被机体的免疫系统排斥，当静脉注射高浓度的腺病毒载体会使肝脏发生严重的炎症反应；非病毒载体目前常用的有脂质体及多聚阳离子聚合物；但脂质体和阳离子聚合物介导基因转移缺乏组织的特异性和靶向性，转染效率较低且易被网状内皮系统吞噬，基因表达时间短；因此研制新型的非病毒载体已成为研究的热点，纳米颗粒具有小尺寸效应，表面效应，随着颗粒直径变小，比表面积将会显著增大，故具有很高的化学活性，因而纳米成为了最有应用前景的非病毒载体。

The virions are released by lysis of the host cell.

通过裂解，可以使新的病毒体从宿主细胞中释放出来。

GPigeon.uac be injected once your computer, under normal circumstances, a variety of the latest anti-virus software is able to Jiesha it, but can not completely remove the Trojan horse "poisonous root," It was because of "gray doves" Of the *. exe files can not be found in the anti-virus software -"dove gray" does not belong to horse-attack from time to time, only every time the computer system to start this normal for a very short period of time attack, the main document to be registered as a Services, will be activated every time the normal release *. dll and * _hook.dll virus, inject them like explorer.exe, iexplore.exe, csrss.exe, lsass.exe process systems, the various anti-virus software can be found Jiesha and in a timely manner, but can not find the root poisoning.

GPigeon.uac被注入你的电脑，一般情况下最近的各种杀毒软件是能够截杀它，但是不能完全、彻底地清除木马的&毒根&，究其原是因为&灰鸽子&的的*。exe文件不能被各杀毒软件查到——&灰鸽子&不属于定时发作型木马，只在每次电脑启动到系统正常这一段很短的时间内发作，主文件被注册成一个服务，每次正常启动将释放*。dll和*_hook.dll病毒体，把他们注入像explorer.exe，iexplore.exe，csrss.exe，lsass.exe等系统进程，因此各种杀毒软件可以查出并及时截杀，但总不能找到&毒根&。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。