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Result The optimum medium formula for protocorm propagation in tissue culture of C. grandiflorium was MS+0.1 mg/L 6-BA. The suitable medium for protocorm induction and differentiation in tissue culture of C. grandiflorum was WS+1.5 mg/L 6-BA+0.3 mg/L NAA with the bud-inducing rate up to 130%. The suitable medium for root inducement was 1/2MS+0.2 mg/L NAA+1.0 mg/6-BA and the survival rate of in vitro plantlets after transplanting was 78%.

结果]大花蕙兰组织培养原球茎粉殖以MS+0.1 mg/L NAA+2.0 mg/L6-BA培养基配方最好;适合大花蕙兰组织培养原球茎诱导分化的培养基配方为MS+1.5 mg/L6-BA+0.3 mg/L NAA,诱导出芽率可达130%;适合大花蕙兰诱导生根的培养基配方为1/2 MS+0.2 mg/L NAA+1.0 mg/L6-BA,试管苗移栽后成活率为78%。

The 1/2 MS medium supplemented with 1.0 mg·L-1 BA and 0.1 mg·L-1 NAA was very beneficial to the protocorm differentiation and propagation of D.candidum .The highest protocorm propagation index was obtained from the medium containing the activated carbon.The highest root numbers and length were observed in plants growing in 1/2 MS medium containing 0.5 mg·L-1 NAA.

结果与结论:添加20%马铃薯液有利于种胚的萌发,种胚萌发率为79.37%;BA 1.0 mg.L-1和NAA 0.1 mg.L-1最有利于原球茎增殖,培养基中添加2%活性碳原球茎增殖倍数高;NAA有助于试管苗的生根,不同含量NAA以0.5 mg.L-1时试管苗平均根数最多和平均根长最长。

Inflorescence development was divided into four stages, the initiating expansion pseudobulb, initiating inflorescence (1-3 cm in flower stalk length), the initial-branching flower stalk, and full bloom inflorescence, respectively. The activity variations of mannan cleaving enzymes,-mannosidase (EC 3.2.1.25), endo--mannanase (EC 3.2.1.78), and the mannan synthesizing enzyme, GDP-mannose pyrophosphorylase (EC 2.7.7.13), were analyzed during different stages to better understand the role of mannan-metabolizing enzymes during inflorescence development of the Oncidium.

将花梗发育过程分为四个阶段,分别为假球茎刚膨大、刚抽梗(花梗长度约1-3公分)、花梗刚分叉以及花盛开,分析参与分解甘露聚醣之酵素-β-mannosidase(EC 3.2.1.25)及endo-β-mannanase(EC 3.2.1.78),与合成甘露聚醣之酵素-GDP-mannose pyrophosphorylase(EC 2.7.7.13),在不同阶段假球茎内的活性变化,了解在花梗发育过程中代谢甘露聚醣之相关酵素所扮演的角色。

The results showed that NaN3 had great effects on TCL growth. Some TCL after treatment got white or browning and died and grew slowly, and the number of regenerated plantlets was reduced, and the regenerated plantlets became abnormal with leaves crimpled and thicker. The RAPD reaction with 10 primers indicated that the molecule maps of RAPD had some polymorphisms which meant the sequence of DNA was changed.

结果表明,NaN3对类原球茎薄切片生长产生重要影响,部分薄切片白化或褐化死亡,再生类原球茎生长受到抑制,再生苗数量减少,部分再生苗畸变,叶片皱缩、变厚。10条RAPD引物的PCR检测发现,再生苗DNA序列发生了一定程度的变异,产生RAPD图谱带型多态性。

Morphological changes during initiation and development of axillary buds and cormels in monocorm and multi-corm taro were studied. Protein content in the top and axillary buds at different developmental stages was analyzed.

观察了魁芋和多子芋腋芽、侧球茎发生发育的形态学变化规律,分析了主球茎顶芽和不同发育期腋芽中蛋白质的组成。

Water chestnut: after photosynthesis, food from the aerial shoot is passed down to the base of the stem and stored at the daughter corms in the preparation for the next growing season.

在一个球茎的营养繁殖:光合作用之后,茎叶所制造的食物被输送到茎的基部,然后在贮藏在子球茎内,以预备下年度的生长。

Methods Clusters of seedlings and protocorms and induction were studied on MS media with different parts of the plant, such as corms, stems, leaves and root tips as explants.

对丽江山慈姑的球茎、地上茎、叶片及根尖等不同部位进行丛生芽及原球茎的诱导,筛选适合生根的培养基配方。

Damaged and diseased bulbs should be discarded. Lay the bulbs out on a screen or newspaper to dry for a few days. Layer the bulbs in a mesh bag or any such ventilated container, and place in a spot where the temperature is around 50 degrees F.

已经受损坏的和有病虫害的球茎应该丢弃,把球茎放在一个筛子或者报纸上经过几天的干燥后,把它们放在网袋或者其他类似能通风的容器内,放置在一个温度在50华氏度左右的地方。

For multiplication of PLBs, the effects of NAA, Sucrose, Maltose, Thiamine HC1, Pyridoxin HC1 were examined by orthogonal rotation combination design.

对于类原球茎的增殖,试验采用二次正交旋转组合设计法探讨了五个因素(NAA、蔗糖、麦芽糖、VB_1、VB_6)对蝴蝶兰类原球茎增殖的影响。

Temperature and solar radiation were less favorable during the whole growing period of taro leaf when transplanting in July to September. The corm dry weight at harvest CDW(subscript HV was linear related to maximum value of LAI LAI(subscript x and cumulative LAI reached 4000℃(CLAI4000). One unit change in LAI resulted in a change of 68g in CDW. One unit change in CLAI4000 resulted in a change of 24g in CDW.

最大LAI值LAI(下标 x及温度总和达4000℃时之累积LAI(CLAI4000)对收获球茎乾物重CDW(下标 HV之影响均呈极显著的直线关系,表示LAI增加速率及最大值愈大,球茎产量则愈高;当LAI提高1单位时,CDW可增加约68公克;当CLAI4000提高1单位时,CDW可增加约24公克。

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