特异

Three genes specified expressed in hybrids and one gene over-expressed in hybrid were found. By cloning, sequencing and BLAST analysis, one specific expression cDNA band was cytosolic isocitrate dehydrogenase gene, the other two were new genes which function were unknown, and the over-expressed cDNA band was proteosome subunit p112 gene.

发现了 3 个杂种鸡特异表达的基因和 1 个杂种鸡表达增强的基因，进一步克隆、测序和比对分析初步表明：杂种特异表达的 cDNA 片断分别为鸡细胞质异柠檬酸脱氢酶基因和 2 个未知功能的新基因；杂种表达增强的 cDNA 片断为鸡蛋白酶体亚基 p112 基因。

Rechallenge was performed 60 days after first tumor inoculation, all mice showed complete protection, indicating that the DC-gp100 induced specific anti-melanoma memory CD8+CTL.

为了验证NV抗原表位可以降低gp100特异的CD8+T 细胞活化阈值，我们先将NV抗原肽负载DC，接着再分别负载不同浓度梯度的gp100抗原肽，以此免疫小鼠，7天后以淋巴细胞增殖实验判断gp100特异的CD8+T 细胞活化阈值。

This report identified S-RNase genes of sweet cherry (Prunus avium L.), presented the sequences of S genes by using a pair of specific primers PruC2 and PruC4R based on the conserved regions C2 and RC4 of Rosaceous S-RNase genes and researched the S gene specific productsfrom the genomic DNAs of different cultivars in which most of the S genotypese were unknown.

根据蔷薇科S-RNase基因高度保守区C2 和RC4区设计一对特异引物PruC2 和PruC4R，对甜樱桃品种的基因组DNA进行S基因特异PCR扩增。

Our results indicate that 1 NSC606985, at nanomolar level, can effectively induce apoptosis in AML cells NB4 and U937 and significantly inhibit the proliferation without cell death in breakpoint cluster region–Abelson murine leukemia kinase-carrying leukemic K562 cells; 2 At such low concentrations, this agent also significantly inhibits the clonogenic activity of hematopoietic progenitors from patients with AML; 3 For apoptosis induction, NSC606985 rapidly induces the proteolytic activation of protein kinase Cδ with loss of mitochondrial transmembrane potential and caspase-3 activation; 4 Co-treatment with rottlerin, a PKCδ-specific inhibitor, completely blocks NSC606985-induced mitochondrial m loss and caspase-3 activation, while the

结果显示：(1)纳摩尔浓度的NSC606985即可有效诱导AML细胞系NB4和U937细胞凋亡并显著抑制含有bcr-abl融合蛋白激酶的K562细胞的增殖；(2)低浓度的NSC606985也显著抑制来自AML病人骨髓的新鲜白血病细胞的克隆形成能力；(3)除了迅速诱导线粒体跨膜电位的崩塌及Caspase-3的活化外，NSC60698也导致蛋白激酶Cδ的水解激活；(4)PKCδ特异的抑制剂rottlerin能够完全抑制NSC606985诱导的线粒体跨膜电位的崩塌和Caspase-3的活化，而Caspase-3特异的抑制剂z-DEVD-fmk仅能部分削弱PKCδ的活化及细胞的凋亡；(5)以移植PML-RARα转基因小鼠产生的白血病细胞建立的模型研究了该化合物对白血病可能的治疗作用。

We had preliminary researched on the mechanism of Stylosanthes guianensis cv. Mineirao resistant to anthracnose, a specific fragment of SG2950-1 was acquired from genome DNA of Stylosanthes guianensis cv. Mineirao by the technique of AFLP in our laboratory, we acquired both ends of the fragment of SG2950-1 by RACE from Stylosanthes guianensis cv.

我们已经对Mineirao柱花草的抗病机理进行了初步研究，并用AFLP分子标记技术从其基因组DNA中获得了一特异片断SG2950-1，利用快速扩增cDNA末端技术将基因组DNA特异片段的两端从Mineirao柱花草扩增出来，经测序后拼接得到MSRA基因的全长序列。

By PCR analysis using specific primers, one specific band at 600~700 bp was distinctively present in cms backcrossed generations. It was genetically stable and cytoplasmically inheritable in the various backcrossed progenies.

根据Brassica nap型细胞质雄性不育相关基因orf222设计兼并引物，利用特异引物PCR方法，分别在不育源、F1、BC2以及BC3世代中扩增出一条600~700 bp 大小的特异条带，而在叶用芥菜保持系中无此条带，具细胞质基因遗传特性。

Amplified fragment length polymorphism was performed on 41 strains of V dahiiae of cotton in search of DNA probes specific for distinguishing between nondefoliating strains and defoliating strains. Specific bands of 433 base pairs and 1 lObp amplified only from nondefoliating strains were obtained using two pairs , E64 M53 and E49 M65 ,which named EM433 and EM110, respectively.

选用25对EcoRI和MseI引物组合，对供试的41个V.dahliae进行AFLP扩增，筛选到两对引物E64、M53和E49、M65，能分别扩增出433bp和110bp两条仅为V.dahliae非落叶型菌系独有的特异片段，可将落叶型与非落叶型菌系分开，这两条特异片段被命名为EM_(433)和EM_(110)。

It was confirmed that facultative apomixes is existed in coffee species based on emasculating treatment, cyto-genetic observe and SSR marker detection.

细胞遗传学观察发现咖啡四倍体类型材料(2n=4x=44)的多胚和多胚苗中含有天然的单倍体胚和单倍体小苗(2n=2X=22)；去雄处理后，可见子房膨大，其中5份材料获得了果实和种子，初步说明无融合生殖现象的存在；对小粒种品种间杂交F1代个体的SSR分析，发现多胚苗中存在与母本图谱相同且不携带父本特异带的后代以及携带双亲特异带的有性后代，表明子代有仅来自母本的无融合生殖个体分离出来。 3 建立了咖啡无融合生殖的分子鉴别方法。

In this study, the author used a molecular marker SCW-37 that was specific for BYDV resistant gene Bdv2, and two molecular markers that were specific for powdery mildew resistant genes Pm13 and Pm21 to analyze 54 candidate plants. As a result, 1 plant pyramided Pm13+Pm21+ Bdv2 resistant genes, 1 plant pyramided Pm13+ Bdv2, 11 plants pyramided Pm21+ Bdvi, were selected for further breeding practice.

本研究利用黄矮病抗性基因Bdv2特异的SCAR标记SCW-37，及对白粉病抗性基因Pm13和Pm21特异的标记，对54株杂交回交后代进行扩增，检测选择到Pm13+Pm21+Bdv_2抗病优质材料1株1484-16；Pm13+Bdv_2一株1484-9；Pm21+Bdv_2基因聚合株11株。

Methods: Sensitivities and specificities of traditional tests, corneal rose bengal/fluorescein staining tests and BUT test were e-valuated in 112 primary SS patients and 185 controls.

在112例PSS患者和185例对照者中，评估上述试验的敏感度和特异度及其主要影响因素。结果：Schirmer、角膜荧光素活体染色和BUT试验的特异度分别为43.8％、93.5％和31.4％，敏感度分别为91.7％、50.0％和87.5％。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。