特异

The results showed that about610 protein spots could be visualized on the two dimensional electrophoresis map bycoomassie brilliant blue staining within Mr 9.0～100.0kD and pH 4～7,and about 94 spotswere differential expressed between male-sterile line and its maintainer line by PDQuestsoftware.Among those differential proteins,about 49 spots and 40 spots were differentialexpressed between uninucleate anther stage and binucleate stage respectively;Meanwhilecomparing with the 2-DE maps of the total proteins from the binucleate anther stage anduninucleate stage,15 differential spots were detectable at male-sterile line;and 16 differentialspots were detectable at its maintainer line.

在分子量9.0～100.0 kD、等电点4～7线性范围内，可识别约610个蛋白质点，PDQuest软件分析结果表明：在单核期不育系和保持系之间存在40个差异蛋白质点，二核期两者共有49个差异点；不育系在单核期和二核期两者之间存在有15个差异点，保持系在两个不同时期有16个差异点；不育系与保持系间在表达量上的差异蛋白质点以及不育系与保持系间特异表达或特异缺失的蛋白质点很可能与小麦雄性不育有关。3。

80 Individuals of ATA-A F2 plants were used for RAPD molecular mapping of the monoecious gene by bulk segregant analysis. 840 random primers were chosen for polymerase chain reaction amplification between monoecious pool and andromonoecious pool, the results showed that: primer S128 was tightly linked to the monoecious character, and primer S493 was tightly linked to the andromonoecious character.

以甜瓜单性花系ATA-A系与两性花品种YJX-1的杂交F2代的80个单株为材料，利用群分法进行单性花基因有关RAPD分子标记研究，筛选分析了840个10bp随机引物的PCR扩增谱带，结果表明，随机引物S128在1180bp，S493在930bp处产生与性别相关的特异性条带，S128的特异条带与单性花性状相关，S493的特异条带与两性花性状相关。

There were on unique different protein observed in flag leaf at different development stage; but unique different protein were found in anther at binucleated stage and the male-sterile materials lack a 31 kD polypeptide compared with normal material.

结果表明，从不同发育时期的旗叶可溶性蛋白中尚未发现特异蛋白的存在，但在花药中发现有特异蛋白的存在，表现在二核期生理型雄性不育界遗传型雄性不育材料均比正常的可育材料缺少了1条分子量为31kD的多肽。

Result 452base pairs DNA fragments specific for Ehrlichia and 555bp for E.canis were amplified from Rhipicephalus sanguineu collected from Guangdong and Boophilus microplus from Guangxi.

结果 从广东采集的血红扇头蜱和广西采集的微小牛蜱样本中扩增出埃立克体452bp特异片段和犬埃立克体的555bp特异片段。

Methods: CD34(superscript +) cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C inhibitor chelerythrine chloride (3 μmol/L) were induced by 5 ×10^(-7)mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR.

采用免疫磁珠法分离人胎肝CD34细胞，培养4d后，蛋白激酶C特异抑制剂chelerythrine chloride(3μmol/L)处理24h，再加入维A酸处理24h5×10^(-7mol/L，无血清培养基培养5d，Western印迹和半定量RT-PCR法分析维A酸处理前后神经特异基因表达。

It was suggested:(1) A strikingly strong sensitivity to eye gaze is observed from birth, and eye gaze perception is key to the development of language capabilities and social cognition;(2) The neural basis of gaze perception is the superior temporal sulcus, which is heavily connected with the amygdala implicated in processing emotion, and the STS is an important part of a wider network for social cognition neural systems;(3) Processing on early stage of eyes gaze cueing reveals specific event-related potential component;(4)The eyes gaze cueing effect has been labeled reflexive and likened to orienting in response to peripheral cues, however, it lasts longer;(5) The gaze cueing effect is influenced by many different facets, from lower level configural aspects such as face orientation to higher level cognitive factors. Furthermore, the gaze cueing effect reveals significant sex difference in normal adult population.

结果发现：婴儿从一出生就对眼睛注视线索表现出强烈的敏感性，眼睛注视知觉对语言和社会等能力的发展有很大影响；(2)颞上沟是加工眼睛注视线索的特异神经结构，它与实时监控情绪和情感的杏仁核存在神经联结，成为社会认知神经系统的重要组成部分；(3)对眼睛注视线索的早期加工显示出特异的脑电活动模式；(4)眼睛注视线索效应与外周线索的反射式效应相类似，但持续时间较长；(5)眼睛注视线索效应不仅受面部结构信息的影响，也受自上而下加工等高水平认知因素的调节，并显示出明显的个体差异。

The confirmative tests of weak-reactive samples included neutralization assay for specific antigen and immune-blotting or immuno-fluorescence antibody assay for specific antibody.

确认方法可分为特异抗原确认的中和试验和特异抗体确认的免疫印迹或荧光抗体试验。

Seven flower/pod-specific genes and one constitutively expressed gene were further investigated.

其中有7种花/豆荚特异的基因和一种特异表达的基因。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶（基因命名 KLK1，酶命名为hK1）cDNA克隆入肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒，用获得的重组质粒转染COS-1细胞，经间接免疫荧光试验证明，上述单克隆抗体能与转染细胞发生特异反应。

In this paper, PCR primers were designed based on the sequence of a single copy in atp6 gene. A 500bp fragment was amplified, and then the PCR products were digested with restriction enzym BglII.

根据atp6的基因序列设计特异扩增引物，以瓣化型胡萝卜核质互作雄性不育系H05A及其保持系H05B为试材，扩增获得了1条约500bp的特异扩增片段。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。