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漏出液

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To complete the surgery, the hole in the dura must be completely sealed to prevent leaking of cerebrospinal fluid.

为了完成整个手术,硬脊膜上的小孔必须完全密封防止脑脊液漏出。

The rat hearts were harvested and mounted on the Langendoff apparatus to perfuse with K-H buffer solution to estimate the leakage of enzymes of the myocardial tissue. Then they were arrested and stored in each preservation solution (4℃) for 8 hours.

采用Langendoff装置进行心脏保存前后的灌注,各实验组分别使用不同保存液灌停心脏并在低温条件下保存鼠心8 h,于灌停前及复灌后留取冠脉流出液测定心肌酶漏出量。

The rat hearts were harvested and mounted on the Langendoff apparatus to perfuse with K-H buffer solution to estimate the leakage of enzymes of the myocardial tissue. Then they were arrested and stored in each preservation solution (4℃) for 8 hours. At the end of the storage period, the rat hearts were resumed and reperfused in Langendoff model. The water content and the leakage of enzymes of the myocardial tissue were measured.

采用Langendoff装置进行心脏保存前后的灌注,各实验组分别使用不同保存液灌停心脏并在低温条件下保存鼠心8 h,于灌停前及复灌后留取冠脉流出液测定心肌酶漏出量。

Methods:LPO content was determined by TBA-Acetic acid method. Samples included healthy serum, plasma, leakage and seepage fluid from thoracicoabdominal cavity and extract materials from cells, plasma before and after taking panax quinquefolium L, serum samples after peroxidation were also taken from healthy patients, coronary heart disease, non-insulin dependent diabetes mellitus, hypertension, edible vegetable oil and its smoke condensation oil.

用TBA-醋酸法检测正常人血清,血浆,胸腹腔渗、漏出液,各细胞提取物及服用西洋参前后的血浆和经过氧化处理后的正常人、糖尿病、高血压、冠心病患者的血清及食用油脂、相应烟雾冷凝油的过氧化脂质含量。

Methods: Leptin levels from serum and ascitic were measured in 67 patients with hepatic cirrhosis (among them complicated with SBP 32 and transudate group 35) by immunoradiometric assay, and the levels of TNF-α and IL-6 were measured by ELISA.

采用放射免疫法测定67例肝硬化腹水患者(SBP组32例,漏出液组35例)血清及腹水瘦素水平,同时采用双抗体夹心ELISA法测定血清及腹水的TNF-α、IL-6水平。

Results 10 example transudate patient according to the traditional method judgment standard examination, some 7 examples is diagnosed correctly, in the peculiarity 7/10(70.0%),22 example percolate, some 13 examples by the right distribution, the sensitivity 13/22(59.0%), the rate of accuracy...

结果10例漏出液病人按照传统方法的判断标准检测,有7例被正确诊断,特异度7/10(70.0%),22例渗出液中,有13例被正确分类,灵敏度13/22(59.0%),准确率为(7+13)/32(65.7%)。

For example, it is often presumed that cardiac ascites is a transudate when this is rarely the case, ascitic protein is .25 g/l in up to 30% of patients with otherwise uncomplicated cirrhosis,32 40–43 and patients with cirrhosis and tuberculous ascites may have a low ascitic protein.44 The serum ascites-albumin gradient is far superior in categorising ascites with 97% accuracy (table 1).42 45 46 It is calculated as

如通常假定心源性腹水为漏出液而这其实非常少见,在达30%的没有并发症的肝硬化病人中腹水蛋白>25g/L,32,40-43有肝硬化和结核腹水的病人也可能腹水蛋白很低。44血清腹水-白蛋白梯度在腹水分类时有更高的优越性,准确率达97%(表1)。42,45-46计算方式如下

Blood D-dimer levels in patients with tuberculous pleurisy,tuberculosis and malignant lesions were higher than normal reference values,with significant difference,while in patients with transudate had no statistical significance with normal reference values.

结核性胸膜炎、肺结核、恶性病变患者血液D-二聚体水平均较正常参考值有显著增高,漏出液患者结果与正常参考值无统计学意义。

The optical density value of cells was detected by MTT colorimetric method, content of MDA and NO in the supernatant fluid of cultured cells was detected by spectrophotometer, and the transudatory content of LDH was detected by automatic biochemistry analyzer.

用MTT法检测细胞吸光度值,利用分光光度计分别测量细胞上清液中的MDA和NO含量。用自动生化分析仪测定培养液中LDH漏出量。

It is very important to biological treatment of angiogenesis diseases such as hypoxic/ ischemic cerebral diseases and cerebral ischemia/ reperfusion.PC 12 cells were treated with cobalt chloride (125uM) for 0、1、 2、4、8、12、16 hours.

离体培养的PC12细胞长满瓶底后,加入终浓度125uM氯化钴的培养液,孵育0、1、2、4、8、12、16h,检测细胞培养上清液中LDH的漏出;并收集缺氧0、1、4、8、16h细胞,戊二醛固定,切片,电镜观察细胞超微结构的改变。

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