测定...的时间

Methods Fifteen dogs were randomly divided into 3 groups. Group A acted as the control group without CPB; group B and group C were subjected to cardiopulmonary bypass and crossclamping the aorta for 90 min. MP or saline was injected intravenously before surgery. Plasma levels of IL-1β、TNF-α、IL-6、IL-8 and IL-10 were measured at the following time points: before MP or saline were administered, aortic declamping, 30 min and 90 min, after aortic declamping. Immediately after the animals was killed, pulmonary arterial rings were obtained and mounted in organ chamber for assessment of endothelial function with endothelium-dependent or non-endothelium-dependent studies.

健康家犬15只，随机分为3组，A组：不进行CPB，作为非体外对照组；B组：麻醉后注射生理盐水10mL，常规全麻和CPB，主动脉阻断90min；C组：麻醉后静脉给予甲泼尼龙5mg/kg，其余处理同B组。B、C组分别在手术前及主动脉开放前、开放后30min和90min 4个时间点采动脉血，放免法测定血浆白介素－1β(IL-1β)、肿瘤坏死因子－α、白介素－6(IL-6)、白介素－8(IL-8)和白介素－10(IL-10)水平；手术结束时，A、B、C 3组均取右肺动脉段，固定于张力传感器，测定血管环内皮依赖性和非内皮依赖性扩张能力的变化。

The control group received same volume of normal saline instead. The platelet, prothrombin time, activated partial thromboplastin time, fibrinogen, factor Ⅷ coagulator activity, the operation duration, and blood loss within postoperative 24 h were determined and compared between 2 groups.

测定两组用药前和术后的血小板计数、凝血酶原时间、部分凝血活酶时间、纤维蛋白原定量、第八因子促凝活性；记录手术时间和手术开始至24 h中出血量，评价其临床效果。

The aim of this study was to explore the potential relationship between the enhancement of instant hemostatic function in vivo of cryopreserved platelets and its procoagulative related molecule activities. The ability of platelet binding factor Ⅴ, density of GPIb-Ⅸ-Ⅴ(CD42a) at platelet member surface were detected by flow cytometry, the clotting time induced by activated platelets were evaluated by coagulometer and platelet count, MPⅤ and PDW were measured by hemocytometer before and after fresh platelets were cryopreserved.

为了探索血小板冰冻保存后膜表面促凝血活性相关分子变化与冰冻血小板体内即刻止凝血功能增强之间的可能联系，用流式细胞术检测了新鲜血小板冰冻保存前后Ⅴ因子结合能力、血小板膜表面GPIb-Ⅸ-Ⅴ分子(CD42a)密度，用血凝仪测定激活血小板诱导血浆凝固时间变化，用血细胞计数仪测定血小板计数、MPⅤ和PDW。

MethodsBasket-stirring method was used to determine the dissolution rate by determining the dissolubility of puerarin at different time.

采用转篮法测定两者的体外溶出度，以葛根素不同时间溶出度为测定指标。

Forty operative cases were randomly divided into the retroperitoneoscopic group and the laparoscope group. All cases were applied general anesthesia, and hemodynamics of 30 minutes after CO2 insufflation, arterial blood gas, time of operation, time of anus purge gas and time of hospitalization were measured.

40例患者随机分为两组，分为经腹膜后组和经腹腔组，每组各20例，均采用全麻，分别测定气腹中30min患者的血流动力学、动脉血气分析及手术时间、术后肛门排气时间和住院时间。

breast cancer cell line MCF-7 were marked killed by trametes robiniphila murr in certain concentration. The IC50 of trametes robiniphila murr in three days to MCF-7 was about 0.05mg/ml. Its cell toxin were increased while the touched time was delayed or the concentration was advanced. Trametes robiniphila murr also can advance cell toxin of adriamycin and reverse resistance of MCF-7/Adr.

用MTT 法测定不同浓度的槐耳浸膏作用不同时间下对乳腺癌细胞系MCF-7 的杀伤作用，同时测定应用槐耳浸膏时对阿霉素的增敏作用和耐药逆转作用；结论：金克在一定范围内对乳腺癌细胞系具有明显的杀伤作用，其作用3 天时，IC50 在0.05mg/ml 左右，随着药物浓度升高和作用时间的延长其杀伤作用相应的增强，对阿霉素化疗具有增敏作用和逆转耐药作用。

The result indicated that the percentage of lymphocyte were decreased, the percentage of neutrophile granulocytes were magnified significantly with the lighting time increasing, which was due to the level of the melatonin inhibited in the long time lighting; and to supply different exogenous dose, induce the percentage of lymphocyte were magnifying, the percentage of neutrophile granulocytes were decreased. And the statistical analysis showed there was significant difference.

结果表明：在给予不同光照时间的条件下，随着光照时间的延长，褪黑素的分泌水平受到抑制，导致具有特异免疫功能的淋巴细胞百分率下降，具有非特异免疫功能的中性粒细胞百分率上升，经统计分析表明不同组间的测定结果均存在显着差异；采用24h全天光照，用以模拟机体切除松果腺的情况，再给予一定剂量外源性褪黑素，导致机体内具有特异免疫功能的淋巴细胞百分率上升，具有非特异免疫功能的中性粒细胞百分率下降，经统计分析表明不同组间的测定结果均存在显著差异，并且高剂量褪黑素对机体的影响更为明显。

By using the silicomolybdic yellow colorimetric method, the paper determines not only the existing form of soluble glass in the newly made-up, being used, and discarded cooling liquid,but also the relation of the stability of cooling liquid to the concentration and standing time of monosilicic acid and total silicic acid. The relation of the concentration of polysil...

本文采用硅钼黄比色法测定新配制、使用中和报废冷却液中的水玻璃存在形态和研究冷却液的稳定性与单正硅酸、总硅酸的浓度和放置时间的相关性；用四氢呋哺萃取比色法测定冷却液中的高聚硅酸含量与放置时间的相关性；研究用超声波、磁场等物理方法处理废冷却液的再生可行性及水玻璃的形态变化。

Dynamic respiratory compliance and total respiratory resistance were measured before surgical incision, after sternal closure in the operating room, and after arrival in the intensive care unit. The following data were recorded: age, weight, preoperative pulmonary infiltrates, preoperative mechanical ventilation, evidence of increased PBF before surgery, duration of CPB, duration of aortic cross-clamp, duration of deep hypothermic circulatory arrest, use of steroids, and volume of ultrafiltrate removed.

分别在麻醉诱导后、手术结束关胸后和返回ICU后3个时间点测定患儿的肺动态顺应性和全肺阻力，并记录患儿年龄，体重，术前胸片浸润性改变情况，术前机械通气情况，术前肺血流量增高情况，体外循环时间，主动脉阻闭时间，深低温停循环时间和体外循环中超滤量等参数。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。