注射

This paper studies the optimum arrangement of gate and vent locations for RTM process design using a mesh distance-based approach and the trigram method to find optimal...

本文讨论了用网格间距法设计最佳注射口和排气口位置，寻找RTM工艺充模过程中的最佳辅助注射口的三线形法以及对多注射口的RTM工艺进行了分析。

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只，随机分为电穿孔组和非电穿孔组，每组9只，制作右下肢趾长伸肌失神经支配模型；EP组为将质粒pEGFP-N1溶液50μl(0.8μg／μl)注射入右趾长伸肌后，立即于两侧腱腹交接处给予电穿孔，电穿孔参数为：电场强度为200V／Cm，脉冲100μs，频率1Hz，施加10次脉冲；NEP组仅质粒pEGFP-N1溶液注射；转染后1、2、3周，荧光显微镜下观察趾长伸肌中GFP的表达情况，转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况，检测和优化体内转染效率。2、健康雌性SD大鼠78只，随机分为失神经对照组、RC2基因治疗组(RC2组)，MAFbx基因治疗组，每组24只，制作右下肢趾长伸肌失神经支配模型，余6只为正常组；分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌，之后给予电穿孔，方法同上；治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化，治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积，肌细胞超微结构变化以及肌纤维中蛋白含量变化。

The toxicity of cryoprotectans increased as the concentration of its increased.3, the survival rate of embryos microinjected with 6M PM was 25.07±1.57% after being coolled in -20℃ for 10 minutes by programmed cooling method, whenas ,the survival rate of controls dealt with five steps balance method was 20.88±2.84%, which indicated that the chilling sensitivity of embryos with microinjection decreased.

而任何一种抗冻剂均随着浓度的增加其毒性随之增加。3、另外，在冷敏感实验中，显微注射6M的PM的胚胎胚胎，应用程序化法处理，以2℃/min的速率降至-20℃，平衡10min后解冻处理，发现注射PM的胚胎低温处理后成活率为25.07±1.57%，而6M的PM五步平衡法同步处理的对照组胚胎成活率为20.88±2.84%，说明显微注射的胚胎冷敏感性一定降低。

The median patient age was 41 years, 1 female and 3 male. The patients received BMSCs infusion at a dose of (1.0-2.0)×107 cells every time by intrabone marrow injection from the anterosuperior iliac spine and BMSCs from the same donor for the same patient were infused more than once.

骨髓间充质干细胞髓内注射具体方法：取右髂前上棘为穿刺注射点，注射骨髓间充质干细胞悬液，4例患者的给药剂量基本控制在(1.0~2.0)×107个细胞/次，行间断多次给药。

Part IIMorpholino-mediated translational inhibition and overexpression oftbx2 in zebrafish cardiogenesis Objective To elucidate the role of tbx2 in zebrafish cardiogenesis via morpholino-mediated translational inhibition and overexpression on the base of expression pattern of tbx2. Methods Gene-specific antisense morpholino oligonucleotides were designed to against ATG region of zebrafish tbx2 to knockdown endogenous tbx2 gene.

设计tbx2-MO和对照MO，在斑马鱼受精卵的单细胞期，进行不同浓度的显微注射，并通过荧光蛋白融合标记实验和tbx2-mRNA、tbx2-MO共注射拯救实验，验证tbx2-MO抑制tbx2基因表达的有效性和特异性；基因过表达是通过给斑马鱼受精卵注射体外合成的tbx2-mRNA，来研究tbx2基因功能获得对斑马鱼胚胎心脏发育的影响。

In Rh2＋DDP group 1000uM cisplatine and 100uM gensenoside Rh2 were injected to nude mice by abdomen. Growth status of tumors were observed. Diameter of tumors were measured. After 60 days,〓Tc-MIBI were injected from vein of nude mice tail to perform SPECT exam and calculate the value R of the tumors. Later the nude mice were killed.

将A549DDP细胞和A549细胞分别种植于裸鼠皮下，种植A549细胞的裸鼠为A549组，种植A549DDP细胞的裸鼠分对照组、DDP组和Rh2+DDP组，每组4只裸鼠。A549组和对照组的裸鼠腹腔注射生理盐水，DDP组裸鼠腹腔注射低效浓度的顺铂；Rh2+DDP组裸鼠腹腔注射低效浓度的顺铂及无毒浓度的Rh2。

IL 1β and IL 6 levels in supernatant of homogenates of cerebral cortices were measured by the Enzyme Linked Immunosorbent Assay.

28只C57BL/6J雄性小鼠接受MPTP 25 mg/kg ip注射连续5 d，对照组（10只）注射生理盐水，在最后一次注射的第1（10只），第3（8只）和第14日（10只）杀鼠，然后用酶联免疫吸附的方法检测大脑皮质匀浆液上清内细胞因子IL 1β和 IL 6的水平。

In other three groups, the left coronary artery was ligated to establish myocardial infarction models. Following 15 minutes of coronary artery deligation, the chest was opened. Rats in the cotransplantation group were treated with 250 μL (1×1010/L) BMSCs suspension + 8 mg/L Salvianolic acid B-treated EPC suspension 250 μL (1×108/L) in five points of the myocardium surrounding the deligation region. Rats in the BMSCs group received 500 μL BMSCs suspension. Rats in the model group were injected with an equal volume of cell medium.

结扎冠状动脉15 min后再次开胸，共移植组吸取骨髓间充质干细胞悬液250 μL(浓度1×1010 L-1)+ 8 mg/L丹酚酸B预处理的内皮祖细胞悬液250 μL(浓度1×108 L-1)，于结扎区附近心肌组织分5点注射；骨髓间充质干细胞组同法注射单纯骨髓间充质干细胞悬液500 μL，模型组注射等量细胞培养液。

We conclude that KB-R7843 has protective effects on contrast media-induced nephrotoxicity. The protective role of KB-R7943 may be related to its positive effects on renal hemadynamics through inhibiting the increase of ET-1 and TXA2 induced by contrast media in the environment of hypercholesterolemia and its cytoprotective influence on renal cells.

据此推导：（1）KB-R7943对高胆固醇血症环境下造影剂注射所致的肾损害有保护作用；（2）KB-R7943对造影剂肾损害的保护作用可能与其阻止高胆固醇血症环境下造影剂注射所致的肾皮质ET-1、TXA2浓度的上升从而阻止高胆固醇血症环境下注射造影剂所致的肾血流动力学的紊乱有关以及其对肾脏细胞的细胞保护作用有关。

Methods: The experimental type Ⅱ diabetic rats were induced by injection of strepiozotocin (STZ, 30mg/kg ) and fed with high fat and high glucose fond. The rats were divided into three groups: a normal conrmal control group, a diabetes group and a treated group. The treated group were hypodermically injected insulin 3U/d at interfemus for 4 months, white DC and NC group were vehicle groups that were treated with the same close saline, By using SABC method, thee express ion of collagen type Ⅰ and Ⅲ in SAN was detected.

高糖高脂饮食加小剂量链脲佐菌素腹腔注射(STZ，30mg/kg)制备Ⅱ型糖尿病大鼠模型，并随机分为正常对照组、糖尿病组和胰岛素治疗组（每只3U/d股内侧皮下注射），对照组和糖尿病组注射等剂量的生理盐水每天1次，持续4个月；窦房结组织行SABC法免疫组织化学显色，检测窦房结组织中的Ⅰ型和Ⅲ胶原的表达及变化。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。