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To test the hypothesis, neontal rat cadiomyocytes Treat with heat shock(HS,42℃,2h) to induce the expression of HSP70,then teated with 0.5mmol/L H2O2. We first found H2O2 can reduced cadiomyocytes apoptosis and HSP70 only over-expression in the HS group, other two group only express HSP70 a little in the test of expression of HSP70.Then we tested the concentration of Ca2+, the [Ca2+ ] of CON was 192.224+6.654, the [Ca2+ ] of H2O2 group was 290.6918+8.922and the [Ca2+]of HS group was 214.2633+4.484.To further determine how HSP70 repaired the Ca2+ homestasis, we measured calcium transient of each group.

实验中应用MTT和流式细胞技术检测细胞的凋亡,发现损伤组的存活率明显低于其他2组,热休克组(Heat shock group,HS group)细胞存活率略低于正常组细胞;免疫组化结果表明只有在HS组才检测到阳性反应,即是HSP70的表达,其他2组均为弱阳性结果;应用离子成像技术对细胞内钙离子浓度进行测定,发现损伤组290.6918+8.922明显高于正常组192.224+6.654和HS组214.2633+4.484,HS组细胞内的钙离子浓度略高于正常组;为了进一步探讨HSP70对于ROS引起的心肌凋亡过程中钙离子的调控机制,又对各组的心肌细胞进行了钙瞬变的测定。

Measure normal early pregnancy choria tissue and JAR cell by RT-PCR, LH/hCG receptor mRNA expression on both pregnancy choria tissue and JAR cell which is consistent with that be measured in ovarian granulosa cells, indicate that JAR cell and normal choria trophocyte presence LH/hCG receptor, hCG is one of the autocrine hormone.

二、用RT-PCR法可测到正常妊娠早孕绒毛组织、JAR细胞的LH/hCG受体mRNA表达,与正常卵巢颗粒细胞所表达的LH/hCG受体mRNA一致,提示JAR细胞与正常绒毛滋养细胞存在LH/hCG受体,hCG为其自分泌激素之一。

Methods The present study included twelve undialyzed ESRD patients with anemia.The sera from the uremic patients were added to CFU-E and BFU-E culture in the concentrations ranging from 1.25% to 5%.In vitro CFU-E and BFU-E growth in the presence of sera from ESRD patients was compared with in the presence normal human subjects with the use of normal mice bone marrows.

选择12例尿毒症肾性贫血未透析患者为试验组,另选正常健康体检者12例为正常对照组,将实验组、正常对照组血清分别稀释成不同终浓度,采用甲基纤维素细胞培养的方法对小鼠骨髓细胞进行培养,对比不同浓度血清组红系集落形成单位、红系爆式形成单位的集落数。

objective to investigate the existence of the erythropoiesis inhibitors.methods the present study included twelve undialyzed esrd patients with anemia.the sera from the uremic patients were added to cfu-e and bfu-e culture in the concentrations ranging from 1.25% to 5%.in vitro cfu-e and bfu-e growth in the presence of sera from esrd patients was compared with in the presence normal human subjects with the use of normal mice bone marrows.results the effects of the sera from uremic patients on cfu-e and bfu-e colony growth in concentrations ranging from 1.25% to 5% were investigated to be dose-dependent.the effect increased with the concentrations increasing of the sera(p<0.05).there were not significant inhibitory effect on the cfu-e and bfu-e growth of mice bone marrow in vitro from sera of the normal subjects.conclusion the erythropoiesis inhibitors exist in uremic sera which has a dose-dependent inhibitive effects on the growth cfu-e and bfu-e of normal mice bone marrow cells.

选择12例尿毒症肾性贫血未透析患者为试验组,另选正常健康体检者12例为正常对照组,将实验组、正常对照组血清分别稀释成不同终浓度,采用甲基纤维素细胞培养的方法对小鼠骨髓细胞进行培养,对比不同浓度血清组红系集落形成单位、红系爆式形成单位的集落数。结果在实验组中,与血清浓度为0的浓度组相比,与各浓度组血清共同培养的小鼠骨髓cfu-e和bfu-e的集落数均明显减少,且随血清浓度的增加,小鼠骨髓cfu-e和bfu-e的集落数逐渐减少,各浓度组间差异有显著性(p<0.05)。结论尿毒症患者血清抑制小鼠骨髓红系祖细胞集落形成,随患者血清浓度的增加,抑制作用增强,具有剂量依赖性。

The term untenantable refers to if there is an interruption of the normal operation of the facilities in the Property, or of the normal supply of water, electricity or gas for over five days or any other circumstances that affect the normal use of the Property.

该无法居住的情况包括在物业内正常的工作被中断,正常的供水,供电和煤气的供应被中断持续五天,或任何其他的情况影响到该物业的正常使用。

The term untenantable refers to if there is an interruption of the normal operation of the facilities in the Property, or ofthe normal supply of water, electricity or gas for over five days or any other circumstances that affect the normal use of the Property.

该无法居住的情况包括在物业内正常的工作被中断,正常的供水、供电和煤气的供应被中断持续五天,或任何其他的情况影响到该物业的正常使用。

The term untenantable refers to if there is an interruption of the normal operation of the facilities in the Property, or of thenormal supply of water, electricity or gas for over five days or any other circumstances that affect the normal use of the Property.

该无法居住的情况包括在物业内正常的工作被中断,正常的供水、供电和煤气的供应被中断持续五天,或任何其他的情况影响到该物业的正常使用。

The pump runs with an abnormal noise, could you check the shaft center lines between the motor and pump to see if they are aliened?

这台泵运转的声音不正常,请检查一下电机和泵的轴线69 这台泵运转的声音不正常,请检查一下电机和泵的轴线是否正常

Self-protect, press the seset button quickly (nit more than 0.1 second), turn on the illumining switch.if the led lighting normally, the system resume normal. if the led light blinks, turn the swith to off, and then press the seset button .if the led light turns dark, the module need to be charged.

模块自保护,可短按顶部的复位按钮(按下时间不能超过0.1秒),打开照明开关,如LED正常发光,即功能己恢复正常,如照明LED不能正常跟光,请先顶部开关拔至&OFF&,再短按复位按钮,如,LED光明显变暗,表示本电能模块需补充电量了。

Results:① The PO2 of model group reduces obviously, but the PCO2 raises obviously, compared with normal contral group having notable difference; The PCO2 of every curing group is lower than the normal contral group equally, the PCO2 overtops the normal contral group obviously, and having notable difference. It explains that the model is maded successful.

结果:①模型组的 PO2明显降低,而 PCO2明显升高,与正常组相比有显著性差异;治疗各组 PO2均低于正常组, PCO2明显高于正常组,且有显著性差异,说明造模是成功的。

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