检测板

At the same time, intact shuttle-plasmid pZ189 was also introduced into the host cell which was used for detection of nontargeted mutation frequency. After replicating in cells, progeny plasmid DNA was isolated and transformed into E coli. MBM7070.In the presence of ampicillin, X-gal and IPTG, pZ189-transformed E. Coli MBM7070 forms blue colony if the plasmid contains an active SupF suppresser tRNA gene, and white or light blue colony if the SupF tRNA gene is inactive.

但是由于检测非定标性突变需同时引入穿梭质粒pZ189在细胞中复制，并回收质粒DNA，通过转化宿主菌E.coliMBM7070，在含x-gal和IPTG的氨苄指示板上培养，如果用于检测突变的靶基因SupF tRNA基因发生突变，则菌落色泽为白色或淡蓝色，而正常者为兰色。

However, experiment indicate that the result of the detection method is inaccurate and anamorphic.

本文讨论了双板分动式侧滑台对轴间侧滑量的检测，在假定条件下，通过数学计算可求出轮间和轴间侧滑量，但实验结果却表明这种检测方法测量结果重复性差、失真较大。

Human umbilical venous endothelial cells were cultured, and were exposed to different concerntrations of HCY with/or not with CuSO4; Cell counting of detached cells were performed with a hematocytometer; the cell survival was monitored by MTT assay and the cellular injury was evaluated by LDH release; Hoechst 33258 staining was used to observe nuclear morphological changes and the apoptosis was measured by DNA agarose gel electrophoresis ;the percentage of apoptosis was quantified by flow cytometry.

体外培养人脐静脉血管内皮细胞，用不同浓度的HCY伴／不伴生理浓度Cu~(2+)与内皮细胞作用；细胞计数板检测脱落细胞率；MTT法及乳酸脱氢酶释放率的测定来衡量细胞的存活与损伤；Hoechst 33258荧光染色观察细胞核形态变化和DNA琼脂糖凝胶电泳检测细胞凋亡，并采用流式细胞术定量测定细胞凋亡率。

Serum estradiol level of rats was detected by means of radioimmunoassay. The positive staining and intensity of lipid in the meibomian gland alveolus was detected by Sudan Ⅲ staining.

实验评估：采用放射免疫分析方法检测血清雌二醇水平；采用组织化学苏丹Ⅲ染色方法对睑板腺腺泡上皮做脂质染色阳性和强度检测。

P19 differentiation was induced by retinoic acid, and P19 cells cultured on gelatin were used as control. Differentiated cells were observed with microscope. TURNEL method and DNA fragmentation analysis were used for apoptosis confirmation. Survival cells were detected by AlamarBlueTM method.

视黄酸(retinoic acid，RA)诱导P19细胞分化，培养于明胶包被培养板上的P19细胞为对照组，普通光学显微镜观察其神经分化情况，TURNEL法和DNA片段化分析法检测细胞凋亡情况，alamarBlueTM染色法检测细胞存活情况。

Companies have major manufacturing equipment: Taiwan Tateyama 12T Lead Machine 3 Taiwan, the United States casting machine Wirtz 9 Taiwan, the United States and Italy MAC production SOVEMA smear machine 4 sets, Japan Yuasa 2V series of four assembly lines, 12V series of assembly line 2 and Note 6 acid machine, cold acid machine 1, import and domestic charger 281 units, as well as atomic absorption Guang-Pu-yee, Miriam large current discharge, high and low temperature thermostat chamber, the functional detection system machines and other advanced testing equipment .

公司主要制造设备有：台湾立山12T铅粉机3台，美国WIRTZ铸板机9台，美国MAC和意大利SOVEMA生产的涂片机4台，日本汤浅2V系列组装线4条，12V系列组装线2条和注酸机6台，冷酸机1台，进口和国产充电机281台，以及原子吸收光普仪、大电流放电仪、高低温恒温试验箱、功能检测机等先进的系统检测设备。

They have tracking function in grinding process, and are able to autocontrol the dimension and process. They can measure, store and transmit static or dynamically for axle journal and dustproof seat information. Automatic measuring machine is adopted to inspect some dimension of the axle, instead traditional manual measuring and it is more credible.

选用进口车轴数控成型磨床及数控外圆磨床，其磨削质量稳定，磨削过程具备跟踪测量检测功能，实现磨削尺寸和过程的自动控制；轴径和防尘板座直径实现静态或动态检测、储存和传输，在线测量。

It consists of belt/roller coater/plate-chain/multi-times chain/trolley conveyer line, package line, varies of special machine, vacuumizing system, working performance test system, safety test system, electronic control system and is a complete set of equipment.

由皮带输送线、辊筒输送线、板链输送线、倍速链输送线、台车输送线、包装线、各种专机、抽真空系统、工况检测系统、安全检测系统、电气控制系统组合而成，形成完整的成套设备。

Quality of sliver forming is tested using Uster AFIS single fiber testing system under different integrated conditions for the rear hood of the card A186F equipped with back teeth guided flat with cotton web cleaner for the 3-step gauges and cotton web cleaner for 4-step wind throughput. Test shows that gauge excess small or excess large will result in poor quality of sliver forming.

为了研究棉网清洁器反齿导向盖板隔距大小和风量大小组合对生条质量的影响，采用Uster AFIS单纤维测试系统，对A186F梳棉机后罩板采用棉网清洁器的3档反齿导向盖板隔距和4档棉网清洁器风量，在不同组合条件下生产的生条质量进行了检测，检测结果显示，过大与过小的反齿导向盖板隔距对生条质量均有不利影响。

Quality of sliver forming is tested using Uster AFIS single fiber testing system under different integrated conditions for the rear hood of the card A186F equipped with back teeth guided flat with cotton web cleaner for the 3-step gauges and cotton web cleaner for 4-step wind throughput.

为了研究棉网清洁器反齿导向盖板隔距大小和风量大小组合对生条质量的影响，采用Uster AFIS单纤维测试系统，对A186F梳棉机后罩板采用棉网清洁器的3档反齿导向盖板隔距和4档棉网清洁器风量，在不同组合条件下生产的生条质量进行了检测，检测结果显示，过大与过小的反齿导向盖板隔距对生条质量均有不利影响。

Methods: Five patients with parkinsonism or dystonia were assigned to general anesthesia using an modified endotracheal tube.

本实验依照人体实验之相关规定进行，五位患有帕金森氏症或肌张力异常的病人接受神经立体定位手术。

If you can benefit from this book, it is our honour.

如果您能从本书获益，这将是我们的荣幸。