根

1H NMR spectroscopic analysis of aromatic protons combined with theoretical analysis of molecular structures proved the existence of two rotational isomers at 300 K, as well as a rapid interconversion equilibrium at 330 K for both compounds Ⅰ and Ⅱ. However, only one conformer exists for compound Ⅲ and puerarin containing 7-phenolic hydroxyl instead of propyl in A-ring as the case of compounds Ⅰ and Ⅱ. Based on UV-Visible absorption data of neutral and basic solutions, and on the density function calculations, the 7-phenolic hydroxyl group in A-ring was found to be more acidic than the 4'-phenolic hydroxyl group in B-ring. The mechanism of derivation reaction and the structure-reactivity relationship of puerarin as an antioxidant were further discussed.

其中7-丙基葛根素Ⅱ属于新型取代衍生物；变温1H NMR和理论计算结果表明，在300 K时，葛根素和4'-丙基葛根素以单一构型存在；而7，4'-二丙基葛根素和7-丙基葛根素则存在两种核磁可分辨的旋转异构体，当温度升至330 K时，两种异构体可以相互转化；通过对比分析葛根素及其衍生物脱质子产物的紫外可见吸收光谱并结合理论计算，确定了A环上7位酚羟基比B环上4'位酚羟基先脱去质子，并由此进一步阐明了葛根素衍生化反应机理以及在抗氧化作用中可能的活性位点和结构活性关系。

Firstly, according to electromagnetic field theory, the 2-dimensional (2-D) mathematical model of a three phase squirrel-cage Y802-2 induction motor is developed. Then general equations can be deduced from finite element discrete and functional analysis. The motor's 2D solving region of electromagnetic field is given based on the boundary conditions and assumption conditions of the squirrel-cage induction motor. Through finite element mothod and inner–outer convergence method, magnetic field distributions and magnetic forces of bars can be calculated in following rotor conditions: health rotor, one single broken bar, two adjacent broken bars, two broken bars with one good interval, two broken bars with two good interval and four adjacent broken bars.

本文首先根据电磁场理论建立Y802-2型三相笼型感应电动机二维电磁场的数学模型，并对其进行了有限元离散以及泛函分析，推导出总体方程；在基本假设和边界条件基础上，给出笼型感应电动机二维电磁场的求解区域，然后应用有限元并结合内外双收敛方法，对样机稳态运行进行数值计算，得出电机在转子完好、一根导条断裂、连续两根导条断裂、间隔一根两根导条断裂、间隔两根两根导条断裂及连续四根导条断裂六种情况下的磁场分布和导条所受电磁力的变化情况。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

The four protozoa had different changing patterns in rhizosphere and nonrhizosphere soil. About 90 times more Colpoda were determined in rhizosphere than in nonrhizospher at 52 days after sowing. There were more Anweba in rhizosphere than in nonrhizosphere at 74 days after sowing. Much more Bodo were found in rhizosphere at 32 days alter corn sowing. However, the number of the Bodo in rhizosphere soil was only 10% of that in the nonrhizosphere soil at 52 days after sowing. Rhizosphere always contains more Cercomonas than nonrhizosphere within the whole growing season of corn.

不同的原生动物的根际效应差异很大，肾形虫在抽雄期R/S比接近90；变形虫在吐丝时期表现出一定的根际效应；波豆虫在大喇叭口时期表现出比较强烈的根际效应，但在抽雄期则表现出十分强烈的负根际效应，根际土壤中波豆虫数量不到非根际土壤的10%；尾滴虫在玉米整个生育时期都表现出微弱的根际效应。

Root-derived organic carbon is composed of three compartments: root detritus, root eaudates and root symbionts, which represents a large important and potential C sink in forest ecosystems, The known responses of root derived organic carbon and heterotrophic microbes to elevated CO2 were reviewed. Overall, increases in root biomass, productivity, and exudation indicate an augmentation of C inputs into the soil via roots, although changes in root lifespan are still unclear and may modify this flux positively or negatively, Altogether, changes in root chemistry and morphology that may augment soil C storage under elevated CO2 include decreased N concentration and wider root diameter, Alternately, a shift in rooting depth upwards in the soil profile may increase average turnover rates of root detritus. Effects of CO2 concentration on C qualities of root exudates and root symbionts are the least understood components discussed here, Changes of soil heterotropic microbial activity and community under elevated CO2 still remained large uncertainties.

林木根源有机C包括根东通过根枯落物、根系分泌物和根共生菌周转3条途径向土壤输入的有机C，它是森林生态系统中一个重要的、潜在的C汇，综述了根源有机C与其微生物对CO2浓度升高的响应，虽然对根系寿命的变化尚不清楚，但CO2浓度升高将导致根系生物量、生产量、死亡量和分泌物的增加；同时，CO2升高亦促使根共生菌生物量的增加而增加了共生菌的C归还潜力，表明CO2升高使根源有机C的输入增加了，CO2农度升高情况下，根系化学性质和形态特征的这些变化均有利于增加土壤C的吸存；而根分布深度的降低则对土壤C吸存不利；CO2浓度升高对根分泌物和根共生菌质量的影响研究则极少，CO2浓度升高下土壤微生物活性和群落组成的变化存在较大的不确定性，目前CO2浓度升高下林木根源有机C对森林长期C吸存的贡献仍很不清楚。

Radiograph of teeth in mesiodistal direction were taken.

结果 ：单根单根管上颌第二前磨牙的根管弯曲仅有 2个，单根双根管牙齿的根管明显弯曲有 6个，在 2 8个双根牙中有明显根管弯曲的 6个，根管弯曲的牙齿总发生率为 6 。6 %(14 /2 13)。

The secondary root number per plant of Yumai No 49,a mi d-s trength quality wheat,is the smallest among the three varieties except the winte r stage but its root system quality is the best;the intensity of reducing TTC an d the activity of SOD of fresh wheat root are the highest during the period fro m winter stage to flag leaf stage.Since flag leaf stage,its physiological charac ters are weaker and have bigger variation during whole stage.The secondary root number per plant of Yumai No 50,a soft quality wheat,is the biggest except duri ng winter stage,and its dry weight of root of single plant is the heaviest in ev ery stage.Before 19th,March,its intensity of reducing TTC is the weakest and dec reases abruptly after flag leaf stage,but its activity of SOD decreases slowly.R oot dry weight per plant of Yumai No 34,a high quality wheat,is the smallest amo ng the three varieties and its root system quality is the weakest.Before jointin g stage,the intensity of reducing TTC is stronger,but after that,it decreases sl owly though it is smaller,and the activity of SOD decreases slowly in every stag e.

中筋小麦豫麦49号除越冬初期外，其它各时期单株次生根条数均为最少，单株根干重介于豫麦34号及豫麦50号之间，单根质量高；越冬初期至挑旗期，根系活力、根中SOD活性均强于其它两个品种，挑旗后，根系生理活性较小，整个生育期根系生理活性变化幅度大；弱筋小麦豫麦50号除越冬初期外，其它各时期单株次生根条数均最多，单株根干重各时期均为最大；拔节前根系活力最弱，挑旗后根系活力下降幅度较大，根中SOD活性较强且各时期变化幅度不大；强筋小麦豫麦34号，单株根干重最小，根系质量最差；拔节前根系活力较强，拔节后根系活动较弱，下降速度缓慢，根中SOD活性各时期变化幅度较小。

Root,immunoassay of ELISA(enzyme linked immunosorbent assay) was employed to determine IAA concentration in root tip, unilateral application of agar block containing IAA on root tip was carried out to investigate the effect of exogenous IAA on phototropic response.

为了研究生长素在水稻根负向光性反应中的作用，用含有IAA的琼脂块贴附在种子根的根尖表面观测IAA对根的生长效应，用ELISA法测定根尖的IAA含量，并对种子根弯曲部分进行半薄切片，结果表明：(1)种子根的生长方向既受光的调控也受外施的IAA的调控，根尖向贴有含IAA琼脂块的一侧弯曲生长；(2 )不定根在光照 1.5h后，背光侧的IAA含量明显大于向光侧；(3)种子根尖发生弯曲生长是伸长区的细胞不均等生长所致。

Ninety seven spores or sporocarps of VAMF were wet sieved from the rhizosphere soil of mycorrhized pteridophytes, from which 25 VAMF species which belonged to the genera of Acaulospora, Gigaspora, Glomus, Sclerocystis and Scutellospora respectively were identified, and the taxonomic characters of the 25 species were described. The Glomus fungi were the dominant VAMF in the rhizosphere soil of pteridophytes, they took up 44% in the identified 25 VAMF (11 species belong to Glomus); the fungi in Acaulospora and Sclerocystis in the next place, they took up 24% and 16% respectively in the identified VAMF; the fungi in the other genera took up 16%. In terms of the relationships of pteridophytes and the VAMF isolated from their rhizosphere soil, there was no specifity between the VAMF and their host.

从具有VA菌根的蕨类植物对应的根际土壤中用湿筛法筛取了97份VA菌根真菌的孢子或孢子果，从中鉴定出了分属于无醒囊霉属、巨孢囊霉属、球囊霉属、硬囊霉属和盾孢囊霉属的VA菌根真菌25种，并对这25种真菌的分类学特征进行了描述；球囊霉属真菌是蕨类植物根际土壤中的优势类群，已鉴定的25种真菌中，有11种属球囊霉属真菌，占44%，其次是无梗囊霉属和硬囊霉属真菌，分别占24%和16%，其他属的VA菌根真菌占16%，从分离鉴定的蕨类植物根际土壤中的VA菌根真菌与蕨类植物的关系看，VA菌根真菌与蕨类植物之间无明显的专一性。

objective to evaluate clinical effect of the handling protaper nickel-titanium rotary instruments in preparation of canals of posterior teeth.methods 96teeth with pulpitis or periapical periodontitis were instrumented by handling protaper rotary instruments using step-down technique,and by k files using step-back technique for control.all teeth were obturated with lateral condensation methods.the efficiency of preparation was analyzed with ra-diographs before and after operation.results no transportation,apical blockage and ledge were found in test.the technique could keep canals smooth.there were two ledge,one instrument fracture and nine transportation in control.the operative time was shorter and post treatment pain seldom occurred in handling protaper group.conclusion the handling protaper niti rotary instruments can prepare the canals effectively and safety.after the preparation,the canals could be easily washed,sterilized and filled.it is an efficient instrumentation method for posterior teeth's canals and be used widely.

目的 评价手用protaper预备后牙根管的临床效果。方法选取患有牙髓炎和根尖周炎的后牙96例，随机分成试验组和对照组各48例，试验组用手用protaper多锥度镍钛旋转器械逐步伸入法预备根管，对照组用k锉，逐步后退法预备根管，两组均用侧向加压充填法充填根管，根据治疗前后的x线片，评价后牙根管预备效果。结果试验组无根管偏移、根尖阻塞、台阶，且根管壁平滑流畅，无器械折断等并发症发生。对照组有2例台阶形成，器械折断1例，根管偏移9例。试验组操作时间短且术后疼痛轻微。结论应用手用protaper预备后牙根管快速、安全，成形效果好，易于根管冲洗消毒及填充，可视为临床上预备后牙根管的有效方法。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。