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Finally,we deal with the stability problem of the Bergman kernel,i.e.,do theBergman kernels of〓converge to the Bergman kernel of D on compact subsets of〓where〓is a sequence of bounded domains converges to a bounded domainD in the sense of Boas?

最后我们研究Bergman核的稳定性问题,即,若一列有界域〓按Boas意义下收敛于一个有界域D,是否有〓的Bergman核在〓上局部一致地收敛到D上的Bergman核?

Finally, we deal with the stability problem of the Bergman kernel, i.e., do the Bergman kernels of D_k. converge to the Bergman kernel of D on compact subsets of D × D where D_k i-s a sequence of bounded domains converges to a bounded domain D in the sense of Boas?

最后我们研究Bergman核的稳定性问题,即,若一列有界域D_k按Boas意义下收敛于一个有界域D,是否有D_k的Bergman核在D×D上局部一致地收敛到D上的Bergman核?

There was no observable morphological change in the macronucleus of the amicronucleate cell during the whole conjugation.

虽然有小核细胞的配子核并不进入无小核细胞,但是两细胞间却有细胞质的迁移与交换。

Mn-complexes in which Mn atom ligand with the N atom within ligand can stimulate the recovery of electron transfer and oxygen evolution. The trinuclear Mn-complex is extremely sensitive to the addition of CaCl2. It is suggested that there is an interaction between Ca2 and carboxyl within the trinuclear Mn-complex during photoactivation and this interaction benefits the ligation of Mn atom to the apo-WOC and form an active WOC. Binuclear MnMn complex shows slightly higher efficiency than binuclear MnMn complex in restoration of O2 evolution activity. It is suggested from our results that recovery of electron transport and O2 evolution with synthetic Mn-complexes is affected by different factors. Cl- can stimulate the reconstitution of WOC at the concentration of over 100mM;the maximal recovery of O2 evolution activity requires the presence of CaCl2 and 33 kDa protein polypeptide together. Bicarbonate can stimulate the reconstitution of WOC.

锰配合物中锰原子与配体中的氮原子配位连接时,能显著恢复电子传递活性和放氧活性;三核锰化合物在重组时对CaCl2的存在非常敏感,我们认为Ca2 与三核锰化合物中的羧基之间存在一定的相互作用,而这种作用有助于锰原子的光配位进而使三核锰化合物易于组装成有活性的水氧化复合物:双核锰化合物MnMn比双核锰化合物MnMn在恢复放氧活性方面更有效;影响锰化合物电子传递能力恢复的因素与影响锰化合物放氧活性恢复的因素不同;在锰蔟重组过程中,氯离子的浓度必须在100mM以上,才能有效进行光重组;最大光重组效率的获得必须有钙离子和33kDa多肽同时存在;碳酸氢根离子促进锰化合物与去锰光系统II的光组装。

With DGD method, the electron microscopy observation has provided new information on the process of chromatin migration. That is: Firstly, the nucleus moved toward cell wall and a picture characteristic of synizesis stage of meiosis appeared; Secdonly, the transmigration of chromatin occured through CC, and a more extensive region constituted of nuclear skeleton left behind chromatin, which was named as"clear spaces" under light microscopy; Thirdly, at the late stage of synizesis, most of chromatin had gone into adjacent cells through CC and fused into a whole, and the nuclear skeleton left in the former cell gradually mixed with cytoskeleton in cytoplasm.

DGD—包埋去包埋电镜观察表明:百合花粉母细胞中有类似核纤层的结构存在;在染色质穿壁运动过程中,首先是细胞核向细胞壁靠拢,并可留下瞬间的运行轨迹——细胞核后方出现只有细胞骨架而少有细胞器的区域;其后是染色质开始穿壁,在穿壁染色质后方细胞核内出现无染色质仅有密集的核骨架的区域,到了染色质穿壁后期,大部分核物质都已穿至相邻细胞,并彼此融合,而残余在原穿出细胞中的核骨架己逐渐与原细胞的胞质骨架融为一体。

An analysis revealed very significant positive correlation between trophozoite area and nucleus area (r=0.54, P .01), trophozoite volume and nucleus volume(r=0.47, P .01), and negative correlation between trophozoite area and trophozoite roundness (r=-0.29, P .01), no significant correlation between nucleus roundness and trophozoite roundness.

相关分析显示滋养体与核的面积(r=0 。5 4 ,P 。0 1)、滋养体与核体积(r=0 。4 7,P 。0 1)相关性有显著性意义,滋养体面积和核圆度呈负相关(r=- 0 。2 9,P 。0 1),滋养体圆度和核圆度之间无相关性。

After HRP application, HRP labelled cells were distributed in the nucleus of trapezoid body , lateroventral periolivary nucleus , retrotrapezoid nucleus , lateral paragigantocellular nucleus and lateral reticular nucleus . The findings demonstrated the existence of neural structural relations between the chemosensitve areas and the Tz, LVPO, NRT, LPGi and LRt, and revealed that these nuclei might participate in the mechanism of central chemical regulation on respiration.

观察到用浸有HRP的滤纸局部敷贴于延髓腹外侧表面化学感受区后,在延髓斜方体核、外周橄榄腹外侧核、斜方体后核、巨细胞旁外侧核和外侧网状核等核团找到HRP标记的细胞,表明上述延髓内部核团与腹外侧表面化学感受区之间存在神经结构联系,提示延髓腹外侧表面化学感受区对H〓等物质的化学感受机制可能与上述核团神经元伸向该区的突起有关。

⑴There are NOS/ER positive neurons in the hypothalamic paraventricular nucleus, supraoptic nucleus, periventricular nucleus and lateral hypothalamic area.⑵The double-labeled neurons were mostly distributed in the medial dorsal and lateral dorsal of supraoptic nucleus and in the medial parvicellular, ventral zone. They were extensive slightly,but scattered in lateral hypothalamic area, sporadic in the periventricular nucleus.

1在大鼠下丘脑视上核、室旁核、下丘脑外侧区及室周核均有NOS与ER双染神经元分布。2 NOS与ER双染神经元主要集中分布在视上核的背内侧和背外侧部及室旁核小细胞部腹内侧区,在下丘脑外侧区分布较广但比较分散,室周核呈散在分布。

In another way, the technology of FQ-PCR (real-time fluorescent quantitative PCR) was used to measure the ratio of white nucleus occupying the whole nucleus in 34 light-yellow strains. Among them, the ratio of yellow nucleus and white nucleus in 19 strains was access to 1:1, while the other strains showed that the copies of yellow nucleus exceeded white ones.

另一方面,本实验利用FQ-PCR(teal-time fluorescent quantitative PCR)技术测定了34个浅黄色菌株中白色核数目占总的核数目的比值,其中有19个菌株白色核的拷贝数与黄色核的拷贝数接近于1:1的关系,15个菌株中黄色核的拷贝数要大于白色核的拷贝数。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。

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Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

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