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In this paper, the effects of enrofloxacin residues on soil ecological processes (soil respiration, soil cellulosic decomposition, soil ammonification , and soil nitrification) were studied.

1μg/ g土对土壤氨化作用有刺激作用,而较高浓度恩诺沙星残留(1μg/ g土,10μg/ g土)则会对其起抑制作用,药物作用活性期为 9d;不同浓度恩诺沙星对土壤硝化作用影响极其显著,当恩诺沙星浓度达到 1μg/ ml时,在 3~ 9d内,对土壤硝化作用有一定抑制作用。

Results The extract of Huanglian Xiangru Decoction with different proportions of herbs could inhibit pathological changes in lung of mice caused by FM1 strain of Influenzavirus A and lower lung index, indicating anti-influenza activities in vivo with antifebrile effects on febrile rats caused by yeast, resistance to acute inflammation of auricular swelling of mice caused by paraxylene, decreasing writhing times of mice caused by glacial acetic acid, and increasing pain threshold of mice caused by hot plate.

结果 不同比例的黄连香薷饮提取物可明显抑制甲Ⅰ型流感病毒FM1株引起的小鼠肺部病变、降低肺指数,有体内抗流感病毒作用,对酵母所致发热大鼠有退热作用,对二甲苯所致小鼠耳廓肿胀有抗急性炎症的作用,有减少冰乙酸致小鼠疼痛扭体次数和提高热板致痛小鼠痛阈值的作用。

Results Job demands were positively related to emotional exhaustion, job resources were negatively related to depersonalization and positively to reduced personal accomplishment.

结果工作要求对情绪衰竭有正向预测作用,工作资源对情绪衰竭和去人性化有负向预测作用、对个人成就感有正向预测作用;工作资源对工作投入的3个维度都有正向预测作用。

It was known that pyridine and Triton-x100 had a bad effect on the cell growth and lycopene production were studied. The results indicated that pyridine and triton-x100 inhibited cell growth and lycopene synthesis;β-ionone increased cell growth and lycopene synthesis when the concentration was 0.05 ml/L, but when the concentration became higher, there would be a decrease on the lycopene output; span-20 increased cell growth and lycopene synthesis effectively when the concentration reached 2.0ml/L, and the lycopene yield became 2.4×10~(-2) mg/L which was 3-fold than control.

研究了培养基中加入不同浓度的小分子效应物吡啶、Triton-x100、Span-20、β-紫罗酮、异烟肼对番茄红素产量的提高作用,结果显示培养基中加入很低浓度的吡啶(0.1ml/L)就会对菌体产生很强的毒害作用;triton-x100对类胡萝卜素的合成也有很强的抑制作用,试验的浓度为0.2ml/L到5ml/L,结果发现它对菌体的生长和番茄红素的合成都有抑制作用;β-紫罗酮在浓度为0.05ml/L时有很小的促进作用,而浓度升高就有了比较强的抑制效果;span-20在液体培养基中的加量为2.0ml/L时,对番茄红素的合成有很强的促进效果,产量达到了2.4×10~(-2)mg/L,比原始菌株产量提高了2倍,而浓度进一步增大就产生了很强的抑制效果。

objective to observe the antitumor activities of different solution extracts of plumbago zeylanica l.in vitro.method mtt method was used to detect the inhibitory effects on four kinds of cancer cells.results chloroform extracts had significant inhibitory effects on the breast cancer cell(bre-04),the nerve cancer cell(n-04) and the lung cancer cell(lu-04),ic50 were 0.2699mg/ml and 0.2634mg/ml,0.4961mg/ml respectively.ic50 of chloroform extract on the hepg2 was 0.9379mg/ml.ic50 of petroleum ether extracts on bre-04,n-04,lu-04 and hepg2 was 0.5902mg/ml,0.5725mg/ml,0.7938mg/ml,0.6374mg/ml;ethyl acetate extracts had fairly inhibitory effects on the lu-04,ic50 was 0.7343mg/ml;n-butanol extracts and water-solubility extracts had notsignificant inhibitory effects on the four kinds of cancer cells.conclusion the antineoplastic effective parts of plumbago zeylanica l.were chloroform and petroleum ether extracts.

目的 探讨白花丹体外抗肿瘤作用的活性部位。方法 mtt染色法对白花丹五种溶剂提取物体外抗肿瘤活性进行了初步的研究。结果氯仿提取物对乳腺癌细胞(bre-04)、神经癌细胞(n-04)、肺癌细胞(lu-04)有较好的抑制生长作用,ic50分别为0.269 9 mg/ml、0.263 4 mg/ml、0.496 1 mg/ml,对肝癌细胞hepg2抑制作用差, ic50为0.937 9 mg/ml;白花丹石油醚提取部位对乳腺癌细胞(bre-04)、神经癌细胞(n-04)、肺癌细胞(lu-04)、肝癌细胞hepg2的ic50分别为0.590 2 mg/ml、0.572 5 mg/ml、0.793 8 mg/ml、0.637 4 mg/ml;乙酸乙酯提取物对肺癌细胞(lu-04)有一定的抑制作用,ic50为0.734 3 mg/ml;水溶性提取物以及正丁醇提取物无明显抑制肿瘤作用。结论白花丹氯仿提取部位、石油醚提取部位体外抗肿瘤作用较好,值得对其提取部位进一步分离纯化并研究其抗肿瘤作用机制。

The biocatalyzed characters of the purified cellulases were investigated with the CMC-Na as substrate. The most appropriate catalyzed temprature for cellulase 1 is 60 "C,and 65 for cellulase 2. The two enzymes have the extreme stability at the temperatures no more than 50.The CMC-Na has the protective effect on the cellulase. The pH stability range of cellulase 1 is 4 ~ 8, and 6-9 for cellulase 2. Zn2+, Ca2+, Mg2+, K+, Li+can ativate cellulase 1. K+, Li"1 can ativate cellulase 2. Cellulase 1 can mainly decompose CMC and salicin. Cellulase 2 can decompose not only CMC and salicin, but other substrates feebly.

以羧甲基纤维素为底物时,酶1的最适催化温度为60℃,酶2的最适催化温度为65℃,在50℃以下稳定性较好,底物对酶有较强的保护作用;酶1和酶2的最适pH分别为5.5和5.5~6.0,酶1的pH稳定性范围为4~8,酶2的稳定性范围为6~9;Zn~(2+),Ca~(2+),Mg~(2+),K~+,Li~+对酶1有激活作用,K~+,Li~+对酶2有激活作用;酶1主要对CMC和水杨素有分解作用,对其它底物几乎不分解;酶2除了可以分解CMC和水杨素外,对其它底物也有微弱的分解作用。

Base on core observation and grain size analysis, and under associated consideration of logging trace characteristics, Chang8 reservoir in study area is classified into three sedimentatal microfacies, subsea river, mouth bar and river flank.3.The mian diagenesis in study area are: mechanical compaction, cementation, metasomasis and denudation in late diagenetic phase.4. In allocation of reservoir macroscopic heterogeneity, more heterogeneticer reservoir, class 1 and class 2, mainly locate in the center of subsea river or crook and divaricate of the river; class 3 and class 4 mainly locate in river flank, and the physical property of these locality are better.5. Diagenesis of reservoir have influence on the pore structure which influences the reservoir microscopic heterogeneity. The better the pore structure is, the slighter the reservoir microscopic heterogeneity is; on the contrary, the reservoir microscopic heterogeneity is serious.

本次研究所取得的主要成果及熟悉有:1、将合水地区长8储层细分为6个小层:长8_1~1、长8_1~2、长8_1~3、长8_2~1、长8_2~2和长8_2~3.2、依据岩心观察和粒度分析,并结合测井曲线特征,研究区长8储层分为水下分流河道、河口坝和河道侧翼四种沉积微相。3、研究区主要经历的成岩作用有:机械压实和压溶作用、胶结作用、交代作用、晚成岩期溶解作用。4、从宏观非均质性平面展布特征来看研究区宏观非均质较强的1类和2类区主要分布于水下分流河道中间或河道拐弯和分又处。3类和4类往则主要分布于水下分流河道侧翼,这些地方储层均质性相对较好。5、储层成岩作用影响储层的孔隙结构,储层的孔隙结构又对储层的微观非均质性有不同程度的影响,孔隙结构越好,则储层的均质性越好,储层的孔隙结构差,则非均质性强。

Results ①Five days after abdominal application of Xiaoji power(0.1 g per mouce),the medicine showed a very significant effect in enhancing gastrointestinal propulsive function.② Xiaoji powder not only had relaxing effect on the normal smooth muscle in vitro,but also had a strong spasmolytic effect on acetylcholine,green bariuminduced spasm of intestinal smooth muscle.The experiment was based on 10 μg atropine sulfate as a positive control to carry out control experiments.It showed the graphics of atropine sulfate in spasmolytic role.③Xiaoji powder navel paste therapy played a positive role in adjusting the gastrointestinal electric disorder caused by deficiency of spleen Qi,and could restore it to its normal state.

结果 (1)以0.1 g/只消积散给予小鼠腹部用药5 d,即显示非常显著的增强胃肠推进功能作用;(2)消积散不仅对正常离体平滑肌有舒张作用,同时对由于乙酰胆碱、氯化钡引起的肠平滑肌痉挛也有强的解痉作用,该实验是以硫酸阿托品10 μg作为阳性对照药进行对照实验,显示了硫酸阿托品的解痉作用图形;(3)消积散敷脐对脾虚引起的胃肠电紊乱有明显的调节作用,使脾虚大鼠的胃肠电基本恢复到正常状态。

Estrogen deficiency can increase the mRNA expression of MMP-1、MMP-3 and IL-1β in OA synovia tissue. Estrogen could inhibit the mRNA expression of MMP-1、 MMP-3 and IL-1β in OA synovia tissue. Progestin could even decrease this expression. Estrogen could slightly increase the mRNA expression of TIMP-1. It is suggested that low dose of estrogen supplement play a protective effect on OA cartilage, and progestin could increase this protective effect.

雌激素缺乏可使OA关节滑膜中MMP-1、MMP-3和IL-1β mRNA表达增加,雌激素对关节滑膜组织中MMP-1、MMP-3和IL-1β mRNA表达有抑制作用,孕激素可增强雌激素的这一抑制作用,雌激素对TIMP-1有轻度升高作用,提示低剂量雌激素对OA关节软骨有一定保护作用,而加用孕激素这一保护作用增强。

Red cell membrane damnified by oxidation can be recovered with LSPC at 5.0 g/mL, the fluorescence polarization degree of the membrane was similar to normal level, indicate that increased anisotropic by membrane oxidation decreased with LSPC.2 Antioxidation Effect of LSPC on skinLSPC can improve body antioxidation ability by increasing the antioxidase synthesis and its activity in skin and serum.

半体内法测得LSPC能显著激活NK细胞活性。表明,LSPC对免疫功能有较强的调节作用。4莲房原花青素对酪氨酸酶及黑色素生物合成抑制作用初步研究 LsPC对黑色素的生物合成有显著的抑制作用。7.8 X10一49/L一5.09几LsPc对酪氨酸酶活力有抑制作用,其中,7.8 X1049/L一0.19/L时,随LsPC浓度增大抑制率下降;0.lg/L~4.og/L时,随着LSPC浓度增大抑制率逐渐增高,最大抑制率高达99.9%,且该抑制作用属于可逆的竞争性抑制。

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