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The concrete work include: First, we consult a lot of herbarium specimen from PE, NAS, IFP and XJA etc.; Second, we collect more than 100 species of Brassicaceae from Xinjiang, Jilin and Shandong; Third, we study the phylogeny of Brassicaceae from China to approach some divergence on the tribe, genera and species of Brassicaceae using many ways including morphology, micromorphology and molecular systematics, furthermore, estimate the significance of every index.

2确定了两种模式植物-拟南芥与盐芥的系统学位置和系统学关系,认为两者分别归于拟南芥属和盐芥属,其亲缘关系较远,拟南芥属靠近须弥芥属、荠属、亚麻荠属、旗杆芥属、无苞芥属、糖芥属以及垂果南芥等,而盐芥属靠近山嵛菜属和沟子荠属,对于它们的深入认识,为两种模式植物在进化领域研究物种抗逆适应与趋异分化提供了一个不可多得的比较模式系统,以及更多可供比较的物种,具有重要的应用价值。

Results The main imaging of adult polycystic kidney disease signs: abdominal plain film (45 cases) showed renal shadow was lobulated increase; cystic wall calcification (14 cases); polycystic kidney disease and renal milk of calcium (8 cases); pelvis angiography (61 cases), the performance of the renal pelvis, renal calices separated, distorted, elongated; retroperitoneal inflatable angiography (6 cases): Performance increases of renal kidney shadow Shadow and the relationship between the neighboring organs, its boundaries clear and identified; CT scan (30 cases): the performance of the majority of the size of the renal low-density areas, ranging from thin-walled circular; B-mode ultrasonography (47 cases) showed a circular echo-free renal multiple classes of liquid dark area .

结果 成人多囊肾的主要影像学征象:腹部平片(45 例)显示肾影呈分叶状增大;囊壁钙化(14例);多囊肾合并肾钙乳(8例);肾盂造影(61例),表现为肾盂、肾盏相互分离、变形、拉长;腹膜后充气造影(6例):表现为肾影增大肾影与邻近器官之间的关系,其界限清晰可辨;CT扫描(30例):表现为肾内多数大小不等的薄壁圆形低密度区;B型超声(47例)表现为肾内多个类圆形无回声的液性暗区。结论影像学检查有助于提高多囊肾的诊断的准确性。

In the long-period toxicity test, DCG was administered repeatedly for 60 days, the dose were 72.6,36.3,18.2 g/kg respectively(It refered to the dose of material,which amounted to 52.6,26.3,13.2 times of 50 kg human clinic use). There is no other abnormal changes about color patem,action,urine and stool,but cibation, hydroposia, body weight and index part of hematological and hematobiochemical parameters,coefficient and histomorphological figure of organs were examined during administration phase.The rats were examined in 21 days after the cessation of DCG, the abnormal changes of index was completely recovered.

定喘颗粒大鼠给药60 d的毒性试验,剂量分别为72.6,36.3,18.2 g/kg(相当于临床50 kg人用量的52.6,26.3和13.2倍),给药期间各组大鼠毛色、活动、二便均无异常变化,但给药组大鼠摄食、饮水、体重以及部分血液学、血液生化学指标和脏器系数有异常变化,停药后,上述异常变化指标均已恢复正常。

Methods MRI-based area measurements through several special coronal levels(anterior commisure, mamillary body and lateral genicular nucleus)of the hippocampus and entorhinal cortex were measured; the using imaging analyzer, we counted neuronal density of the hippocampus and entorhinal cortex on the same coronal levels.

应用MRI与组织学测量相对照的实验方法, MRI测量20例无明显神经系统疾病的人脑,在通过几个特定冠状层面上(前联合、乳头体及外侧膝状体),测量海马、内嗅区皮层厚度及面积;组织学方面应用图像分析仪进行神经元计数。

Their behaviour,activity,hair,feces were not found abnormal.Their food-intake and body weight were without any obvious variation;(2) After the experiment periods of three months and six months,the hematology examination of rabbits in all Ogroups and in all B groups was not made with distinct difference;(3) The blood biochemical measurement values in Ogroups and in B groups were basically similar;(4) When the systematic thanatopsia of laboratory rabbits,one rabbit each in a high-dose O group and a B group demonstrated 2-3 yellow-white circular nodes as large as a green bean in lingula pulmonis sinistri.Ohter organs were not found of obvious pathologic changes;(5)With the histopathology examination,the pathologic change of lung tissue seem by naked eyes was a focal suppurative inflammation under microscope.

其行为、活动、被毛、粪便等未见异常,进食量及体重变化未见明显差异;(2)实验期3个月、6个月的血清学检查,各药膏组与空白药膏组比较无显著差异;(3)药膏组与空白药膏组的血液生化测定值基本一致;(4)各药膏组实验兔的系统尸检表明,用药期空白药膏组和高剂量药膏组各有一只实验兔的肺心叶有2~3个黄白色绿豆大小圆形结节,其他脏器未见明显病变;(5)病理组织学检查,肉眼见到的肺组织病变镜检为局灶性化脓性炎症,其他组织未见病理性改变。

By using the several neuroradiologic skills, we can be offered the richable, nonaggressive, direct information to the clinical and experimental neurology researcher, make progresses of the two courses tightly.

利用多项神经影像学技术,优化检查序列,可为临床及实验性脑卒中研究提供丰富的、无创性的、直接的信息,使神经科临床治疗与影像学诊断紧密地结合起来。

Results 1. The expression of P53, KI-67, hTERT and ToPo II a protein was higher in astrocytoma than that in nomal brain tissue, the expression of these markers was obversely correlated to the grade of astrocytoma (P.05). The expression of Fas was higher in astrocytoma than that in nomal brain tissue, on the contrary, expression of P16 was lower in astrocytoma than that in nomal brain tissue.

结果 1、星形细胞瘤中P53、KI-67、hTERT与ToPoⅡα蛋白表达阳性率高于正常脑组织,P53、KI-67、hTERT与ToPoⅡα蛋白表达与星形细胞瘤的病理组织学分级呈显著正相关(P.05);Fas蛋白在星形细胞瘤中的表达高于正常脑组织,而P16蛋白表达低于正常脑组织,Fas、P16蛋白表达与组织学分级无显著相关性。

The periostea of both experimental and control side of the mandibular ramus were taken and prepared, 2 of each 5 rabbits in a group were prepared for HE stain detection and 3 for proliferating cell nuclear antigen immunohistochemical detection.Results:1, The newly formed bone was detected on the lateral aspect of mandibular ramus after periosteal distraction. The bone was shaped like a hill. It looked very low and was full of holes at postoperative day 28. With the time of consolidation period lengthened, the newly formed bone matured gradually. X-ray examination showed the new bone shaped like a hill. The average values of new bone height at postoperative days 28,35,42 and 56 were 1.86 + 0.15mm, 2.29 + 0.29mm,3.19 + 0.13mm and 4.70 + 0.45mm. Histological examination of both HE stain and picricacid-fuchsin stain showed the increase in the number of osteoblasts and the change in the orientation of collagen fibers and bone trabecula. There were no significant differences between newly formed bone and original bone on the lateral aspect of mandibular ramus at postoperative day 56 histologically.2 Compared with the control side, the distracted periostea proliferated obviously under the microscope, and the number of periostealcells increased with satiation of cellular nuclear per unit area. The images of PCNA immunohistochemical stain of periosteum showed that the experimental periosteum proliferated obviously after distraction compared with the control side.

结果:骨膜牵张成骨的实验研究南京医科大学硕{学位论文l、骨膜牵张后,可见下领升支外侧的骨皮质上有新骨形成,新骨呈山峰状凸起,术后第28天的新生骨较低平,多孔隙,随着固定时间的延长,新骨逐渐成熟;下领升支前后向切线位X线投照显示新骨呈山峰样隆起;经测量,术后第28、35、42和56天组平均新生骨厚度分别为x.86士0.15mm、2.29士0.29mm、3.19士0.13mm和4.70 土0.45mm;脱钙骨组织的HE染色和不脱钙骨组织的苦味酸一品红染色的组织学观察均显示了新生骨在成骨细胞数量上的增长,以及胶原纤维和骨小梁排列方向上的变化,术后第56天的新生骨在组织学上与原升支骨组织已无明显区别。2、HE染色显示,与对照组相比较,实验侧骨膜增生明显,细胞间排列紧密,单位面积内骨膜细胞数增多,细胞核饱满;骨膜PCNA 免疫组化染色显示,与对照侧相比较,实验侧骨膜在牵张后出现了明显的增生迹象,PCNA阳性细胞分布紧密,单位面积内阳性细胞数较对照组多,靠近骨表面的骨膜中的阳性细胞数更多而且分布更为紧密。

The author could observe the dissolved degeneration of part of sacrospinal muscle fibers,muscle fiber hypertrophy,and a small quantity of inflammatory cell infiltration with a light microscope.

结果]各项酶学检验无异常;组织学光镜下观察到竖脊肌纤维部分溶解变性,肌纤维肥大,少量炎性细胞浸润;电镜下观察到肌纤维灶状溶解,核周线粒体聚集,胞内脂滴、溶酶体增多,肌卫星细胞增殖分化。

Result]All of enzyme tests were normal.The author could observe the dissolved degeneration of part of sacrospinal muscle fibers,muscle fiber hypertrophy,and a small quantity of inflammatory cell infiltration with a light microscope.Focal solution of muscle fiber,the aggregation of mitochondria around the nucleus,the increase of lipid droplet and lysosome in cyte,and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope.

结果]各项酶学检验无异常;组织学光镜下观察到竖脊肌纤维部分溶解变性,肌纤维肥大,少量炎性细胞浸润;电镜下观察到肌纤维灶状溶解,核周线粒体聚集,胞内脂滴、溶酶体增多,肌卫星细胞增殖分化。

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