斑马鱼

The third part of this study is mainly on Ecsit in zebrafish embryogenesis.

本论文的第三部分研究了Ecsit在斑马鱼早期胚胎发育中的功能。

In zebrafish, the expression of fez becomes detectable at the anterior edge of the presumptive neuroectoderm by 70% epiboly.

本文用整体原位杂交的方法揭示了在斑马鱼胚胎发育过程中，Fez基因的表达最早出现在原肠期胚胎头部未来的神经外胚层细胞中，此时胚盘仅下包约70％。

Recently, there is already known Pregeneolone (P5) which could affect epiboly movement of zebrafish embryos is the first product of the steroidogenesis pathway, but effects of further downstream products on epiboly movement are unknown.

目前研究已知固醇荷尔蒙生合成机制的第一个产物pregnenolone (P5)对斑马鱼胚胎epiboly 移动有影响，但其它下游的固醇荷尔蒙对epiboly移动的影响仍然未知。

A genechip analysis was performed using RNAs derived from embryos injected with squint mRNA, MZoep mutant embryos that are deficient in Nodal signaling, and wildtype embryos at the 30% epiboly stage Transcriptswith at least two-fold changes in expression level between wildtype and the other samples were identifyied In squint mRNA-injected embryos, 265 transcripts show anincreased expression level and 111 have a decreased expression level; in MZoep embryos, the expression of 1 495 transcripts increases while 550 transcripts express at a decreased level.

为鉴定受Nodal信号调控的基因，特别是那些转录因子基因，通过将来自squint过量表达、缺失Nodal信号的MZoep突变体或野生型30%外包期胚胎的RNA与Affymetrix斑马鱼寡核苷酸芯片杂交。

Besides its convenience used in development research, the application in ethology is more and more extensively.

斑马鱼在神经生物学中的应用，除了在发育方面比其他模式动物更具优势外，在行为学方面的应用也更加丰富。

Using the zebrafish as an animal model, researchers in the lab of Harvard Medical School professor of systems biology Timothy Mitchison and Dana Farber Cancer Institute professor Thomas Look have discovered that when the tail fins of these creatures are injured, a burst of hydrogen peroxide is released from the wound and into the surrounding tissue.

利用斑马鱼作为动物模型，哈佛医学院的研究员生物系统学教授Timothy Mitchison和Dana Farber癌症研究所的Thomas Look教授发现，当这些生物的尾鳍受伤时，大量的过氧化氢被从伤口释放出来，进入了周围的组织。

Forced expression of fez induces ectopic expression of dlx2 and dlx6, two genes involved in brain development.

通过显微注射含有Fez基因的DNA或mRNA使该基因在野生型斑马鱼胚胎中超表达，可以诱导两个与脊椎动物前脑发育相关的基因dlx2和dlx6发生提前和异位的表达。

This result showed that the nucleotide sequence of the coding region and accordingly amino acid sequences of Myf-5 gene of gayal and cow, chimpanzee, rhesus monkey, human, dog, house mouse, Chinese softshell, chicken and zebrafish were quite conservative.

大额牛与普通牛、黑猩猩、恒河猴、人、狗、家鼠、软体贝壳、鸡、斑马鱼的Myf-5基因编码区核苷酸序列同源性分别为99.0％、90.0％、90.5％、90.5％、87.2％、84.8％、72.9％、64.3％、51.8％，相应的氨基酸序列同源性分别为99.6％、94.9％、94.6％、94.9％、89.9％、88.7％、81.6％、70.4％、56.6％，这说明大额牛与其他9个物种在Myf-5基因的编码区核苷酸和相应氨基酸序列上具有较高的保守性。

Some problems about the evolution of the repeated DNA in human Y chromosome-specific heterochromatin region were discussed.

因此，以斑马鱼和河豚等鱼类模式物种与人类之间直接开展比较基因组研究是近年来的国际学术探讨热点之一(Amores et al 。，1998； Meyer et al 。，1998)。

Whole-mount in situ hybridization showed that zebrsfish rnf141 gene expressed ubiquitously during embryogenesis, with higher expression found in the telencephalon, cerebellum and hindbrain.

杂交结果显示，rnf141基因在斑马鱼胚胎中广泛表达，尤以端脑、小脑和后脑表达丰度为高。

Methods: Five patients with parkinsonism or dystonia were assigned to general anesthesia using an modified endotracheal tube.

本实验依照人体实验之相关规定进行，五位患有帕金森氏症或肌张力异常的病人接受神经立体定位手术。

If you can benefit from this book, it is our honour.

如果您能从本书获益，这将是我们的荣幸。