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Additionally,this study developed three species-spcific real-time fluorescence quantitative PCR-based assays for rapidly, specificity, sensitivity,detecting Brucella abortus, Brucella melitensis, Brucella suis.

在建立布鲁氏菌属荧光定量PCR检测方法的基础上,根据布鲁氏菌基因组多拷贝插入元件IS711及其下游多态位点,设计了检测牛、羊、猪种布鲁氏菌的引物和牛、羊、猪种特异性荧光探针,优化了牛、羊、猪种布鲁氏菌荧光定量PCR反应体系和条件,建立了牛、羊、猪种布鲁氏菌荧光定量PCR检测方法,组装了牛、羊、猪种布鲁氏菌检测试剂盒,该方法能特异检测上述3个种的布鲁氏菌。

The preparation of polymer/montmorillonite intercalation composite materials has been studied by applying two kinds of photo polymerization reaction (photo acid generation and photo radical generation)in this work.

用小角X光衍射技术对产物内蒙脱土的结构进行表征,结果表明,蒙脱土经改性后能很好地使聚合单体及预聚物插入黏土层内并在光照下"就地"聚合生成光聚合复合材料。

Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库,在大肠杆菌中扩增后提取混合质粒DNA,经电转化非缺陷标志酒精酵母 AS.2.1364,在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子,从阳性转化子中分离重组质粒证实含5.0kb的插入片段,用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

The results show that the Zn complex can be partially intercalated into the double helix of DNA due to the planarity of the benzimidazole ring and the bulky structure of the complex.

实验结果表明,由于配合物的八面体构型以及苯并咪唑环的平面性,其与DNA作用方式可能为部分插入。

An aerosol spray device which is capable of imparting a unipolar charge by double layer charging and charge separation to liquid droplets of a composition sprayed therefrom has a spraying head in the form of an insert in an actuator, the spraying head having a bore through which liquid is expelled having an outlet, preferably with a tortuous periphery, having an L/a ratio of at least 8 (preferably at least 10) where L is the length of the periphery defining the bore outlet in mm and a is the cross-sectional area of the bore outlet in mm2 and the apparatus being constructed such that the droplets are expelled from the spraying head at a flow ratio of at least 0.4 (preferably at least 0.5) grams per second and have a charge to mass ratio of at least +/-1x10-4 C/kg.

本发明还提供了一种喷射装置,它能够通过双层充电和电荷分离而向要喷出的组合物液滴施加单一极性的电荷,该喷射装置具有一个喷头,该喷头制成为致动器中的插入件的形式,喷头具有一个孔腔,液体能够通过该孔腔从装置中排出,该孔腔具有一出口,该出口最好具有曲线周边,并且L/a比率至少为8,其中L是以mm为单位的确定孔腔出口的周长,a是以mm2为单位的孔腔出口的横截面积,该装置构造成使得液滴在至少每秒0.5克的流动速率以及具有至少+/-1×100C/kg的电荷/质量比的状态下从喷头排出。

Visible'.'' through a haze or thin cloud, especially such a ring around the moon or sun, caused by diffraction of light from suspended matter in the intervening medium.

微弱'。''颜色的发光的环,尤指围绕月亮或太阳的这种环,是由于悬浮物在插入媒介中的衍射造成的

Have had the intense function fromthis the explanation preparation and DNA, matched the body the planearyl residue groups and bases to insert the DNA double spiral plane,caused the plane aryl residue groups and bases the electronic clouddensity to have the change.

由此说明配合物和DNA发生了强烈作用,配体的平面芳基基团插入了DNA的双螺旋平面,导致平面芳基基团的电子云密度发生改变。

Someone – etcetera etcetera etcetera – had jammed a bottle between them in a rather rude position.

某个人—或者某物等等等等,在那两条腿之间以相当粗鲁的姿势插入一个瓶子。

During the Late Triassic to Early Jurassic, a sequence of continental margin sediments and flysch were highly deformed and thrust eastward onto the rocks of the Yangtze craton while the Sichuan Basin was accumulating clastic sediments as a foredeep basin.

边缘沉淀顺序和复理层高度不成形,并向东插入到长江克拉通的岩石之上;与此同时,四川盆地不断地沉淀可分解的沉积物而形成了前渊盆地。

These experiments suggest that the aromatic moiety on the side chain of the ligands may partially intercalate into the base pairs of DNA.

说明这三个配合物是以配体支链上的芳香环平面部分地插入到相邻碱基对之间与DNA结合的。

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