插入物

These effects may be due to frequent reactivation of the active center for blocky copolymer by hydrogen.

我们推测这可能是由于H_2对发生丙烯二级插入的嵌段性活性中心频繁活化的作用，导致原来不加氢时生成嵌段共聚物转化为加氢下生成无规共聚物。

These kinds of complexes have steady thermodynamics property and abundant information of photochemistry and photophysics,which can bond with DNA by the mode of insert,static,chimb-face combine.In this paper,bipyridyl was selected as ligand.

金属吡啶配合物与DNA相互作用的研究一直是生物无机化学领域十分活跃的研究课题，此类配合物的热力学性质稳定，光化学和光物理信息丰富，能够以插入、静电和沟面结合三种方式与DNA作用。

In the process of use, the external hook will insert rope or other falling objects, when pick up drilling tool, hook tooth hooks falling objects and take to the ground.

在使用中将外钩插入绳类或其他落物内，上提钻具时，钩齿钩住落物而带到地面。

Methods Forty-four EBTB patients weredivided into an experiment group and a control group. All the patients of the two groups were treated with generalchemotherapy, the patients of experiment group accepted endobronehial decimeter wave therapy at the same time. Thepurulent secretion or caseous necrosis of the diseased region was drawn-off from the bronchi lumina by bronchoseope,then the antenna of the decimeter wave irradiator was inserted into the diseased region through the biopsy pore to per-form the radiation.

共选取44例狭窄型支气管内膜结核患者，将其随机分为治疗组及对照组。2组均给予常规抗结核药物治疗，治疗组患者同时介人纤支镜分米波治疗，将纤支镜插入病灶支气管内，充分吸出病灶支气管管腔内脓性分泌物或干酪样坏死物后，经纤支镜活检孔插入分米波天线，并将天线尖端插入病灶内实施分米波辐射。

Six pairs of oligonucleotide primers were designed to amplify and sequence scarlet gene of mutant stbr flies which were obtained by outcross between Bar and wild type flies.

设计了6对引物对本实验室由棒眼果蝇Bar与野生型果蝇杂交所产生的亮红眼突变型果蝇stbr的scarlet基因进行了测序，结果在scarlet基因的第4外显子检测到了一个7.5kb的插入，进一步的测序证实此7.5kb插入为逆转座子412元件。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Correlation coefficient: 0.9962 . Positively charged'free'complex ions should be readily quenched by 〓, but when it is bound to DNA, it can be protected from the quencher because the highly negatively charged 〓 would be repelled by the negative DNA phosphated backbone, hindering quenching of the emission of the bound complex.

从理论上讲，带正电的配合物的阳离子应该可以被大阴离子淬灭，但是在DNA存在下，金属配合物插入到DNA的碱基对中，而〓被带负电荷的DNA磷酸骨架强烈排斥，从而避免了〓的淬灭，因此大的Stern-Volmer曲线斜率相应于对配合物较弱的保护形式。D。

Full length nucleic acid sequence of recombinant pGEM-AC-7 were determined and we found recombinant pGEM-AC-7 was 1146 bp long, inculding all open reading frame except initiation coden ATG and 192 bp 3-end non-coding reagion.

DNA序列分析和PCR鉴定表明，插入片段均由5&端特异引物单个引物扩增出来，对7号重组克隆插入片段DNA全序列分析表明，7号重组克隆插入片段全长1146 bp，包含了除启始密码子外的全部编码区和192 bp的3&端非编码区。

A method and device for continuously supplying drops into female moldings which are rotatingly movable for manufacturing moldings, wherein synthetic resin in molten condition extruded from an extrusion opening is cut by a cutter attached to a holding mechanism to form the molten resin into drops in a determined quantity, the drops are held and conveyed by the holding mechanism, and the drops are forcibly inserted and supplied into the concaves of the female moldings.

是一种将用于对成形品进行成形的滴物向旋转可动的形成阴模连续供给的方法及装置，将从挤压开口部挤压出的熔融状态的合成树脂通过附设在保持机构上的切断工具切断、形成定量的滴物，利用保持机构将滴物保持输送，向成形阴模凹部强制插入、供给。

The method is just like the QuikChange site-directed mutagenesis method except for its special primer. This pair of primers is composed of a long primer and a short primer which is central overlapped.Both of the Tm of 5\'sequence in long primer and Tm of 3\'sequence in short primer are above 66℃.The Tm of short primer is almost half of that of long primer.It is convenient to perform insertion or substitution mutation of DNA fragments larger than 20bp,In addition,it is highly applicable in research about the deletion mutation of DNA fragments larger than 2000bp.

我们实验室一直在寻找一种简单高效的可以用于基因的删除、插入及替代突变的方法，通过大量研究和实验发现，可以使用一种基于反向PCR原理，使用全新的引物设计的突变方法——&COP&突变法，该方法采用与QuikChange点突变法相同的操作步骤，其策略是设计一对中心重合的引物，长引物的5\'端序列和3\'端序列的Tm值均不低于66℃，短引物Tm值约为长引物一半，且与长引物中心重合互补。

Objective To investigate the effects of interleukin-1 β converting enzyme gene on the biologic characteristics of ovarian cancer cells.

目的 探讨白细胞介素-1 β转换酶的表达，对卵巢癌细胞生物学特性的影响。

Campylobacter: This illness is the most commonly identified cause of diarrheal illness in the world.

弯曲：这种病是最常见的原因查明腹泻病，在世界上。