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Methods Realtime fluorescence quantitive RTPCR was used to detect the expression level of Tiam1 mRNA in gastric cancer tissues of 66 cases, paracancerous tissues (over 3cm) of 66 cases and gastric benign lesion tissues of 11 cases. Meanwhile, CK18 was selected as the hallmarker for lymph node micrometastasis to detect the level of CK18 mRNA expression in 199 lymph nodes from 24 cases of gastric carcinomas diagnosed negative by pathologic examinations.

采用TaqMan探针实时荧光定量PCR方法,定量检测66例胃癌患者癌组织、癌旁组织及11例胃良性病变组织中的Tiam1 mRNA拷贝数,同时将CK18作为淋巴结微转移的标志基因,定量检测24例病理诊断无淋巴结转移的胃癌患者199枚淋巴结的CK18mRNA拷贝数。

Indeed, copies flow so freely we could think of the internet as a super-distribution system, where once a copy is introduced it will continue to flow through the network forever, much like electricity in a superconductive wire.

实际上,拷贝流的自由流动让我们可以把互联网想像成一个超级的配送系统,一旦产生了一个拷贝,它将永远在网络中流动,就像超导导线中的电流。

Commercial radio and TV provide an "existence proof" of a working superdistribution system in which the copies are disseminated free and the stations only pay for what they use.

同样,商业电台和电视台也为这种有效的超级散布提供了一个&存在性证明&。因为拷贝的流传是免费的,而电台和电视台只要为它们所用到的那些拷贝来付钱就好了。

Commercial radio and TV provide an "existence proof" of a working superdistribution system in which the copies are disseminated free and the stations only pay for what they use.

商业电台和电视台为超级分发系统提供了&存在性证明&。该系统自由分发拷贝,电台和电视台只为它们使用的那些拷贝付钱。

Methods Endogenous EB1 mRNA and protein levels in embryonic cells of trophonema from artificial abortion and spontaneous abortion were compared, using fluorescence quantitative PCR, immunohistochemistry and western blot, respectively.

结果 EB1基因mRNA拷贝数/GAPDH基因mRNA拷贝数的均值在人工流产绒毛核型分析正常的胚胎细胞中为0.01004±0.008223,在自然流产绒毛核型分析异常的胚胎细胞中为0.1509±0.0633。

Storage is physically copied only as a consequence of modifications (to the source, or to the copy if the copy is writeable).

存储的物理拷贝只发生在修改之后源数据被改变,或者拷贝数据可写并被改变。

The digital watermarking technique, which is a new technique for protecting the copyright of the digital products occurring in recent years, is a hot point of the research of international academic circlers at present.

近年来,数字化技术和Internet的飞速发展,在最大限度地拓宽权利人利益范围的同时,也带来了版权保护的危机。由于图像、视频、音频和其他作品都能以数字形式获得,其拷贝非常容易,从而可能会导致大规模非授权拷贝,而这极有可能会损害音乐、电影、书籍和软件等出版业的发展。

The results indicated that the detected MDV1 in feather tip was the most abundant in all of samples (10^-10^8 copies per million cells). The FQ-PCR had a higher positive rate (100%) compared with AGP and regular PCR methods, and its sensitivity was 10-100 times than that of regular PCR, and the lower detection limit of the MDV1 assay was 2.78 copies.

结果表明,不论实验攻毒鸡还是自然发病鸡,羽髓中病毒富含量均高于其它组织,每百万宿主细胞内病毒含量为10^7~10^8拷贝;FQ-PCR检侧MD发病鸡只的阳性率高于AGP,达100%;该方法的灵敏度比常规PCR检侧高10~100倍,在单位细胞内可灵敏地检测到2.78个拷贝的病毒。

A ''Transparent'' copy of the Document means a machine-readable copy, represented in a format whose specification is available to the general public, that is suitable for revising the document straightforwardly with generic text editors or generic paint programs or some widely available drawing editor, and that is suitable for input to text formatters or for automatic translation to a variety of formats suitable for input to text formatters.

文件的&透明&拷贝指的是一份机器可读的拷贝,它以一种一般公众可以取得其规格说明的格式来表现,适合于直接用一般文字编辑器、一般点阵图像程序用于由图元像素构成的影像或一些可以广泛取得的绘图程序用于由向量绘制的图形直接地进行修订;并且适合于输入到文字格式化程式,或是可以自动地转换到适合于输入到文字格式化程序的各种格式。

A ''Transparent'' copy of the Document means a machine-readable copy, represented in a format whose specification is available to the general public, that is suitable for revising the document straightforwardly with generic text editors or generic paint programs or some widely available drawing editor, and that is suitable for input to text formatters or for automatic translation to a variety of formats suitable for input to text formatters.

文件』的「透明」拷贝指的是一份机器可读的拷贝--它以一种一般公众可以取得其规格说明的格式来表现,适合于直接用一般文字编辑器、一般〔点阵〕图像程序(用于由图元像素〔pixel〕构成的影像)或一些可以广泛取得的绘图程序(用于由〔向量〕绘制的图形)直接地进行修订;并且适合于输入到文字格式化程序,或是可以自动地转换到「适合于输入到文字格式化程序」的各种格式。

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