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Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示:脾脏PCR检测结果表明1、2两实验组均有阳性病例出现,而对照组全部为阴性,说明实验鸡体内已有ALV-J内蒙株感染;接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化:肝脏、脾脏有程度不同的肿大,镜下观察,骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生,随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶,而对照组没有出现明显病变;免疫状态方面:以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强,ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

The part one of this thesis was concerned with expression and purification of recombinant low molecular weight sweet potato PAP and generation of a polyclonal antiserum from it.

本论文的第一部分致力于对低分子量的红薯紫色酸性磷酸酶的表达和纯化的研究,并产生相应的抗血清。

Antibody of mouse against rat of glial fibrilla acidic protein used to labeling astrocytes of the brain and antibody of rabbit against rat of c-fos labeling c-fos protein in the brain. The second fluorescence antibodies were FITC and Cy3, respectively. Observations of the results and quantitative analysis were conducted with LSCM.

在本实验中,我们应用免疫组织化学的双标方法,用小鼠抗大鼠胶质原纤维酸性蛋白标记大鼠脑内的星形胶质细胞,用兔抗大鼠c-fos标记大鼠脑内的c-fos蛋白,分别应用FITC和Cy3标记的荧光二抗来显示,最后用激光扫描共聚焦显微镜进行观察并定量分析。

The present study explored the effects of morphine on plasticity of spinal cord in three parts as follows: 1. the effects of morphine on dorsal root terminal in spinal lamina Ⅱ of spared root rat---- a quantitative study by FRAP histochemistry; 2. the effects of morpnine on synaptic reinnervation in spinal lamine Ⅱ of spared root rat----an EM quantitative study and 3. the effects of morphine on collateral sprouting of nerve fiber in spared root of rat----an EM quantitative study

本课题从三个方面探讨了吗啡对脊髓可塑性的影响:1、吗啡对备用根大鼠脊髓Ⅱ板层背根终末的影响——抗氟化物酸性磷酸酶组化定量研究;2、吗啡对备用根大鼠脊髓Ⅱ板层突触重建的影响——电镜定量研究;3、吗啡对大鼠备用根神经纤维侧支出芽的影响——电镜定量研究。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of MCSF (25μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 μg/L) and M-CSF (25 μg/L) with or without 10 μg/L TNF-α for4, 5, 6 and 9 days. The number of TRAP multinucleated cells and resorption pits on dentine slices were counted under light microscope.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞,在含有25μg/L M-CSF和0,1,10,100μg/L TNF-α的α-MEM培养液中培养5d后,观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成;细胞在含有25μg/L M-CSF和30μg/L sRANKL的α-MEM培养液中进行培养,比较加入和不加入10μg/L TNF-α培养4、5、6和9d后,所形成的TRAP多核细胞的数目和骨吸收面积。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of M-CSF (25 μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞,在含有25μg/LM-CSF和0,1,10,100μg/LTNF-α的α-MEM培养液中培养5d后,观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成;细胞在含有25μg/LM-CSF和30μg/LsRANKL的α-MEM培养液中进行培养,比较加入和不加入10μg/LTNF-α培养4、5、6和9d后,所形成的TRAP多核细胞的数目和骨吸收面积。

The biochemistry and image analyzing were used to investigate the effects of the PDGF-AA at high concentration on the osteoclastic bone resorption in the osteoblast-osteoclast co-culture system.

在高浓度PDGF-AA的作用下,应用生化检查和图象分析技术,检测PDGF-AA对成骨细胞-破骨细胞共育体系中破骨细胞骨吸收功能的影响;应用免疫荧光检测破骨细胞合成抗酒石酸酸性磷酸酶的变化;应用免疫组化和RT-PCR技术检测骨保护素配体的表达。

Results of this study indicate that alginate fiber can not resist acid and alkali, both salt endurance and oxidation resistance of alginate fiber are worse; various factors were applied to research the weight-lost of the fiber, such as concentration,temperature and time.

根据印染加工的特点,对海藻酸纤维的耐酸性、耐碱性、耐盐性及抗氧化性进行了初步研究与探讨,为海藻酸纤维在纺织行业的开发与应用提供了理论基础。

Results of this study indicate that alginate fiber can not resist acid and alkali, both salt endurance and oxidation resistance of alginate fiber are worse; various factors were applied to research the weight-lost of the fiber, such as concentration,temperature and time.

论文摘要:根据印染加工的特点,对海藻酸纤维的耐酸性、耐碱性、耐盐性及抗氧化性进行了初步研究与探讨,为海藻酸纤维在纺织行业的开发与应用提供了理论基础。

Methods: Cells isolated from neonatal rat long bones attached to matrix or sedimented onto bone slices and were stained with Gimsa and for tartrate-resistant acid phosphatase .

从新生SD大鼠的股骨、胫骨和肱骨的骨髓腔面分离粘附性强的细胞,直接在倒置显微镜下观察其活体形态或者经Gimsa染色后观察其形态;用抗酒石酸酸性磷酸酶染色对其进行鉴定。

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