抗抗酶

Earthworms might be a good bioindicator for the contamination of roxarsone to soil. No significant influence of roxarsone contamination upon the biomass and the reproductive ability of earthworms was found, which indicated that earthworms could tolerate heavy contamination of roxarsone. 2. Effects on the important enzymatic system of earthworms The activities of PROD, BROD and MROD in P450 family were inhibited.

对蚯蚓体内重要酶系统的影响洛克沙胂对P450酶系中的PROD（7-戊氧基异吩噁唑酮-O-脱乙基酶）、BROD（7-苄氧基异吩噁唑酮-O-脱乙基酶）和MROD（7-甲氧基异吩噁唑酮-O-脱乙基酶）的活性都有抑制作用，对MROD的抑制作用最明显；在抗氧化酶系统中，对过氧化氢酶在整个试验进程中都有激活作用，而对其他抗氧化酶有时起激活作用，有时起抑制作用，规律性不明显。

Vulgaris to magnetic treatment The Superoxide anion in algae cell increased and antioxidant ability was declined by a suitable magnetic treatment. There was different response of the enzymic component-Superoxide dismutase , Catalase , and peroxidase enzyme, and their activities were induced to increase. There was no much response for the nonenzymic component-glutatnione to the magnetic treatment, while content of carotenoid was reduced remarkably with the increasing magnetic intensity and time.

小球藻抗氧化系统对磁处理的响应一定强度的磁处理使小球藻中超氧阴离子·O〓升高，抗氧化能力降低，抗氧化酶系统的关键酶SOD，CAT，POD酶应激性升高；非酶体系的GSH对磁处理响应不明显，Caro含量普遍有较为明显的降低，随处理时间的增加Caro的量下降显著。

The activities of anti-oxidases of Alternanthera philoxeroides were much higher than that of Orgza sativa, the activities of SOD, POD and CAT increased significantly in Alternanthera philoxeroides, while they changed a little in Orgza sativa.

在渗透胁迫下，空心莲子草体内的SOD、POD、CAT等抗氧化酶活性及总抗氧化能力均显著上升，而水稻的抗氧化酶活性变化相对较小。空心莲子草的抗氧化酶活性显著高于水稻。

SOD and POD were important antioxidant enzymes for resisting drought in seedlings of Broussonetia papyrijera L., which can effectively avoid the iniury of oxidative stress for the trees through inducing the plasma-membre protection system. But the function of CAT to resist drought was not obvious.

SOD和POD是构树幼苗抗干旱的重要抗氧化酶，它能够通过自身诱导抗氧化酶系统以提高适应能力而忍受逆境，但构树幼苗的CAT酶在抗干旱胁迫上的作用不明显。

In order to understand antioxidant enzymes activities in response to temperature increment and the active oxygen free radical clearing function of antioxidant enzymes in antarctic ice mircoralgae Chlorophyceae L4, the free radical generation rate, the malonaldehyde content changes and the corresponding antioxidant enzymes activities changes in Chlorophyceae L4 at different temperatures were determined.

为了阐明南极冰藻Chlorophyceae L4抗氧化酶活性对温度升高的响应以及了解冰藻细胞内抗氧化酶系统对活性氧自由基的清除作用，对不同温度下（包括最适温度6℃、高于最适温度10℃和15℃）Chlorophyceae L4细胞中自由基的产生速率、丙二醛含量的变化和相应的抗氧化酶系统活性的变化进行了测定。

The major results of this proposal are as follows:(1) ABA induces a rapid, substantial accumulation of apoplastic H2O2 in mesophyll and bundle sheath cells of maize leaves, and the accumulation of apoplastic H2O2 is involved in the induction of the chloroplastic and cytosolic antioxidant enzymes.(2) ABA-induced H2O2 production activates a 46 kDa MAPK, which in turn induces the expression of antioxidant genes and up-regulates the activities of antioxidant enzymes. The activation of MAPK also enhances H2O2 production, forming a positive amplification loop.(3) Water stress also induces the activation of a 46 kDa MAPK, which is dependent on the accumulation of ABA and H2O2 production induced by water stress and involved in the up-regulation of the expression and the activities of antioxidant enzymes.(4) ABA-induced H2O2 production mediates NO generation, which in turn activates a 46 kDa MAPK and results in the up-regulation in the expression and the activities of antioxidant enzymes in ABA signaling. NO-independent signaling is also involved in ABA- and H2O2-induced antioxidant defense.

本项目的主要研究结果如下：（1）ABA诱导的H2O2产生主要出现在叶肉细胞与维管束鞘细胞的质外体中，质外体H2O2的积累能够上调叶绿体与细胞溶质中抗氧化酶的活性；（2）ABA诱导的H2O2产生活化一个46kDa的MAPK，由此而导致编码抗氧化防护酶基因的表达与酶活性的上调；而MAPK的活化也能增强H2O2的产生，从而形成一个正的反馈调节环；（3）水分胁迫也能诱导一46kDa MAPK活化，这一MAPK活化依赖于水分胁迫诱导的ABA积累以及H2O2的产生，同时参与水分胁迫诱导的抗氧化防护基因的表达与抗氧化酶活性的上调；（4）ABA诱导一个依赖于H2O2的NO产生，NO活化一个46kDa的MAPK，从而导致抗氧化防护基因的表达以及抗氧化酶活性的上调；同时一个不依赖于NO的信号转导途径也存在于ABA诱导的抗氧化防护过程中。

Methods Anti-DNase B antibodies of 87 patients with acute rheumatic fever and 202 rheumatic heart disease patients with rheumatic activity were detected with anti-DNase B antibody microtitration test,and compared with antistreptolysin "O" test.

采用抗DNA酶B微量法和自制试剂测定急性风湿热87例、活动期风湿性心脏病202例病人血清的抗DNA酶B抗体，与抗溶血素O抗体对照研究。

Objective To evaluate diagnostic value of anti-deoxyribonuclease B microtitration test in rheumatic fever. Methods Anti-DNase B antibodies of 87 patients with acute rheumatic fever and 202 rheumatic heart disease patients with rheumatic activity were detected with anti-DNase B antibody microtitration test,and compared with antistreptolysin "O" test.

目的 评估A组溶血性链球菌感染的血清学测定方法对诊断风湿热的临床价值方法采用抗DNA酶B微量法和自制试剂测定急性风湿热87例、活动期风湿性心脏病202例病人血清的抗DNA酶B抗体，与抗溶血素O抗体对照研究。

The activities of superoxide dismutase, catalase, peroxidase and malonyldialdehyde were determined in hepatopancreas in oriental weatherfish Misgurnus anguillicaudatus exposed to different concentrations 0.025mg Cd(Ⅱ/L, 0.25 mg Cd/L and 2.5 mg Cd/L of Cadmium chloride Cd(Ⅱ to evaluate the effects of cadmium stress on xanthine oxidase and antionxidant enzyme activities.

研究了不同浓度的cd对泥鳅肝脏内抗氧化酶包括超氧化物岐化酶、过氧化氢酶、过氧化物酶活性的影响以及对脂质过氧化指标－丙二醛含量的影响，探讨了在Cd胁迫下，泥鳅肝组织内抗氧化酶活性以及脂质过氧化的变化趋势及其关系。

Methods: Bypreparing anti-human type Ⅳ collagen IgG with rabbits and utilizing sodium periodate horse-radish peroxidase-labelling anti-human type Ⅳ collagen monoclonal antibody to change diluentcomponent and concentration, we establioshed sandwich ELISA.

以免疫家兔制备抗人COLⅣ多抗包被微孔板，采用改良过碘酸钠法辣根过氧化物酶标记抗人COLⅣ单抗，改变各种稀释液成分及浓度，建立双抗夹心酶免疫测定法。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。