微探针

ABSTRACT: Objective To investigate the influence of glutamate levels in rat striatum after intracerebral implantation of microdialysis probe.

目的 探讨微透析探针置入的刺激对大鼠纹状体内谷氨酸水平的影响。

According to the advance of research of tradescantia ecotoxicology all the world, this paper summarized the advance of research and significance of new problem of Tradescantia micronucleu mechanisms and tradescantia stamen hair mutation mechanisms, and the development of the probe test technology, quantification test technology and tradescantia ecotoxicogenome and so on in order to give a reference to research the tradescantia molecular toxicology.

文章从国内外紫露草生态毒理的研究现状出发，针对紫露草微核产生及雄蕊毛突变机制这类新问题的研究现状及意义，以及探针检测技术、定量检测技术和紫露草的生态毒理基因组学的发展等方面进行综述，为紫露草分子毒理上的研究提供参考。

The phase equilibrium relationship and the equilibrium composition of conjugate phases in Ag--Ti system were determined by means of metallographic microscope and electron probe microanalysis. Especially for the liquid phase the B phase and the intermediated phase.

通过金相观察和电子探针微区分析，对退火处理后的试样进行相分析和成分测定，从而获得了相应温度下的Ag--Ti二元体系的相平衡关系和共轭平衡成分。

This review focuses on newly developed strategies for miRNA quantification, and elucidates in detail the probe-hybridization based methods including Northern blotting, microarray, gold nanoparticle labelling, and splinted ligation with radioactive labels. The amplification-based methods including quantitative PCR, rolling cycle amplification, invader assay, and the next generation sequencing methods were also discussed. The advantages and disadvantages of these methods were compared.

文章系统地介绍了最新发展的microRNA定量检测方法，详细阐述了基于探针杂交技术的Northern blotting法、微阵列芯片法、纳米金标记法、桥连同位素标记法，以及基于扩增技术的定量PCR检测法、滚环扩增法、引物入侵法和新一代大规模高通量测序法等，并对这些方法的优缺点进行了分析比较。

Both of the two methods are compatible with selective liquid phase epitaxy.

这样使得选择液相外延方法制备的微探尖也可以应用于其它种类的扫描探针显微镜。

First we give the analysis of the receiver front-end block diagram, link budget and specification allocation. Then, all the sub-circuits , including low noise amplifier, waveguide-to-microstrip transition , image reject band--pass filter, low-pass filter, and sub-harmonic mixer , are described in details.

在接收机组成方案分析，链路计算及指标分配的基础上，结合理论计算和计算机仿真，给出了各单元电路(包括探针式波导-微带转换器，低噪声放大器，镜频抑制带通滤波器，分谐波混频器，低通滤波器等)的设计和仿真结果。

The probe of chip includes the entire genomic DNA of at least ten thiobacillus ferrooxidans, at least two thiobacillus acidophilus sulfur oxides, at least one eosinophils thiobacillus, at least one acidithiobacillus albertensis bacteria, at least six thiobacillus oxidation of ferrous - Lo, at least eight heterotrophic bacteria acidophilus, at least one metal leaf sulfur bacteria, at least one Acidianus tengchongensis, at least a Metallosphaera sedula and at least two of thermophilic bacillus curing.

该芯片的探针包括至少10株氧化亚铁硫杆菌、至少2株嗜酸氧化硫硫杆菌、至少1株喜温嗜酸硫杆菌、至少1株Acidithiobacillus albertensis菌、至少6株氧化亚铁微螺菌、至少8株异养嗜酸菌、至少1株金属硫叶菌、至少1株嗜酸两面菌、至少1株勤奋金属球菌和至少2株嗜热硫化杆菌的全基因组DNA。这种芯片，能够检测酸性环境中丰度较低的微生物，具有高通量、同时、快速、准确、灵敏地分析酸性环境中微生物群落组成的优点。

Cytoplasmic actin microfilament systems were localized in the freshly isolated onion protoplasts of bulb parenchyma cells using TRITC labelled phalloidin probe in the present study.

使用四甲基罗丹明标记的鬼笔环碱探针，以新分离的洋葱鳞茎薄壁细胞原生质体为材料，观察了细胞胞质肌动蛋白微丝骨架的结构与形态。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

In the case of analytical protein microarrays, well-characterized molecules with specific activity, such as antibodies, peptide-MHC complexes, or lectins, are used as immobilized probes.

就分析的蛋白质微阵列来说，具有特定活性的很好描绘的分子，如抗体，肽-MHC复合物，或凝集素，被用作固定的探针。

Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来，英国，在此地早期居住的是凯尔特人，已经被征服了三次。

Bluetooth OBEX File Transfer Enables the sending and receiving of files on your phone via Bluetooth.

蓝牙OBEX文件移动允许经过蓝牙传送和接受文件。。。。