嵌合

The leafstalk, chief vein, bud, the cane top on side branch, bast and xylem of type B had type A and C tow components, belonging to chimeric tissues. The leaf pulp and thin leaf vein had no components of type C, belonging to pure tissues of type A. Type B plant had two kinds of color roots, the rufous roots belonged to type A tissue, the yellow roots belonged to type C tissue through the SSR identification.

深州杨B型的叶柄、叶主脉、芽、侧枝茎尖、韧皮部、木质部同时检测到深州杨A、C型两种成分存在，属于嵌合组织，B型叶肉及细叶脉中检测不到深州杨C型成分，属于深州杨A型纯合组织；深州杨B型植株的地下部分含有红褐色和土黄色两种根，经检测，红褐色根是属于深州杨A型的纯合组织，土黄色根是属于深州杨C型的纯合组织。

The new chimeric infectious DNA clone and its derived, a virulent chimeric virus are constructed from the nonpathogenic PCV1 in which the immunogenic ORF gene of the pathogenic PCV2 replaces a gene of the nonpathogenic PCV1, preferably in the same position.

所述新型嵌合的传染性DNA克隆及其衍生的有毒力的嵌合病毒是用非致病性PCV1构建的，其中，致病性PCV2的免疫原性ORF基因取代了非致病性PCV1的基因，优选是在相同的位置上取代。

To develop safe and effective vaccines is the fundamental strategy to combat the fatal disease. Among the HIV vaccines in research, live attenuated vaccine is attractable since it can induce broad, long-lasting and strong immunity.

采用HIV与猴免疫缺陷病毒(SimianImmunodeficiency Virus，SIV)构建的SHIV嵌合病毒为开发HIV疫苗提供了一个新方向，但由于SIV与HIV的同源性很高，二者嵌合的SHIV在安全性方面存在一定的风险。

The characteristics of the grafted "Improved Sweet Orange" seedlings showed various segregations, such as "yellow fresh color of Aurantium type","chimaera of red or yellow fresh color of Aurantium type","red fresh color of Aurantium type","chimaera fruit of 1Vlicroacrumen type" and "variant of Microacrumen type", in the vegetative progenies found so far.

一种是把植物的嫁接变异视为&嫁接杂种&；另一种看法则认为除非两个嫁接亲本体细胞的核真正融合在一起，成为染色体数加倍的新个体，才能称为&嫁接杂种&，而这种&嫁接杂种&至今还未见到有可靠的报道。目前所见到的有关嫁接变异的报道，实际上是一些&嫁接嵌合体&，即两个嫁接亲本体细胞的机械嵌合。

The two disease-resistance genes chitinase and glucanase,which were respectively directed by commelina yellow mottle virus promoter and CaMV35S promoter,were introduced into cotton genome via Agrobacterium tumefaciens .

通过根癌杆菌介导法，将维管束特异启动子(竹节花黄斑病毒启动子CoYMV)启动的几丁质酶和CaMV3 5S启动的β 1，3 葡聚糖酶嵌合双价基因，导入陆地棉品种冀合 3 2 1和中棉所 3 5。

The two disease-resistance genes chitinase and glucanase,which were respectively directed by commelina yellow mottlevirus promoterand CaMV35S promoter,were introduced into cotton genome via Agrobacterium tumefaciens.

通过根癌杆菌介导法，将维管束特异启动子(竹节花黄斑病毒启动子CoYMV)启动的几丁质酶和CaMV35S启动的β-1，3-葡聚糖酶嵌合双价基因，导入陆地棉品种冀合321和中棉所35。

Results The expression plasmid of pVAX1 IL-18 HN was contructed successfully confirmed by enzyme digestion identification. The expressions of IL-18 and HN chimeric genes were confirmed, and the expressed HN protein had higher hemagglutinative titer.

结果：酶切鉴定证实正确地构建了pVAX1 IL-18 HN嵌合表达质粒，Western blotting和血凝试验检测结果表明，嵌合后的IL-18和HN基因均能够表达，且表达的HN蛋白具有较高的血凝活性。

After being induced to differentiate in vitro, cells with various morphologies showed β-gal activity, including nerve cells, neuroglial cells, epithelial cells. We also detected β-gal activity in a wide variety of tissue elements in solid tumors made by injecting the MC15 and MA34 cells into syngenic mice. Then we produced chimeric embryos by injecting the MC15 cells into Kunming blastocysts and recovering the embryos at various developmental stages.

SiCMVIE(Simian Cytomegalovirus Immediately Early)启动子是一个强启动子，本文首次将SCMVIE启动子应用到ES细胞途径上制作嵌合鼠，并通过lacZ基因在ES细胞和早期嵌合胚胎中的表达，表明该启动子是一个能在小鼠多数组织表达的启动子，这与1993年Peppel等人用原核显微注射法获得的转基因小鼠上所得的有关结果是基本上是一致的。

Methods: The human Fd repertories genes were amplified by RT-PCR from PBMC of hepatoma patients, and cloned into the vector pComb3X with chimeric L chain to construct the human-mouse hybrid Fab phage library.

利用RT-PCR自肝癌患者外周血淋巴细胞中扩增全套人抗体重链Fd基因片段，克隆入含嵌合L链的展示载体pComb3X，建立人－鼠杂合Fab噬菌体抗体库。

Chimeric analysis of the thymuses and spleens by FACS at day 1 and day 30 after separation demonstrated that the survivals of skin allografts were not correlated with the chimeric degrees.

利用流式细胞仪分析技术分别对上述处理的BALB／c及B6小鼠在联体分开后的第1天和第30天进行胸腺及脾脏嵌合程度的检查，发现皮肤耐受期与嵌合程度不呈正相关。

Objective: To study the effect of polycythemia on blood oxygen saturation.

裴蕾目的：观察RBC剧增而引起的高粘血症对血氧饱和度的影响。

Based on SIMPLER algorithm in the curvilinear body-fitted coordinates, the calculations were performed for Pr=0.7, Re=10~1000 on non-orthogonal non-staggered grids which are generated by elliptic equation systems.

采用曲线坐标系下压力与速度耦合的SIMPLER算法，数值研究了周期性渐扩渐缩波纹通道内脉动流动与换热情况，流动Re数的范围为10~1000，Pr数为0.7。