嵌合

So it can be essential momitoringindex of colon carcinoma and its tumor specificness is apply by manygene therapy of tumor. Transcriptional regulatory element of CEAcontains enhancer element and promotor in the 5th flanking region whichcan control expression of objective gene in positive cancer tissue of CEA. Objectives: To explore the joint promoter with high activity,consisting CEA promotor and CMV enhanser.

目的：本课题探讨由CEA启动子、CMV增强子组成的高活性嵌合启动子，通过融合启动子的调控提高融合自杀基因yCDglyTK在靶细胞表达的特异性；并以无毒副作用生物相容性好的磷酸钙纳米为载体，将该自杀基因靶向导入结肠癌细胞中，以达到提高基因治疗靶向性及安全性的目的。

Aim To prepare sterically stabilized liposomes modified with chimeric TNT-3 monoclonal antibody （chTNT-3） and investigate their immunoreactivity and in vitro targeting.

目的 研究人鼠嵌合抗肿瘤细胞核单抗（chTNT-3）-空间稳定脂质体的制备方法、免疫活性及体外靶向性。

Experiment Ⅴ: Mouse polyploid embryos were produced by electrofusing 2-cell blastomeres successionally.

实验六、通过2-细胞卵裂球交换制作2n／4n嵌合小鼠胚胎，对这种胚胎的发育能力进行了评价。

In this thesis, a multi-block grid method using embedding technique is adopted and applied to the computation of the transonic flow about wing-body-pylon-nacelle configuration.

本文对使用嵌合技术的三维多块网格方法计算多部件外形的跨音速流动进行了比较深入的研究。

Hereby, we will take advantage of trimera model to evaluate the effect of the new kind of gene carrier on tumor therapy and explore the possibility of applying the model to evaluate human-based vaccines.

因此，本研究拟利用这种人-鼠嵌合模型来评价我们所制备的新型基因疫苗载体在肿瘤治疗中的作用，探讨用这种模型来评价基于人的各种疫苗的可能性。

In recent years, major progress in the research of humanized animal model has been achieved. As same as transgenic animals, trimera model has become a candidate for evaluating the effect of vaccines targeting to human.

近年来，人-鼠嵌合模型在人源化动物模型研究领域中取得了很大进展，并使其和转基因动物一样，成为评价疫苗效果的一种选择。

Keywords: pancreatic cancer ;vaccine;trimera

中文关键词：胰腺癌；疫苗；三重嵌合

The involvement of oxidization of peroxid in the antigen-antibody reaction makes the cell membrane permeability enhanced, so the fluorescence-labeled antibody stain can infiltrate into the cell easily and contact the antigen rapidly and diffusely, and then the antigen-antibody crosslinking can be formed efficiently.

利用过氧化氢的氧化作用参与抗原抗体反应，使细胞的膜通透性增强，利于荧光标记抗体染液渗入，从而更快速、广泛地接触抗原，更有效的抗原抗体的嵌合交联。

Necropsies were performed at 21 days post-challenge, the lymph nodes in the nonvaccinated but challenged pigs were larger than those of vaccinated pigs. The PCV2 genomic copy loads in lymph nodes were reduced in vaccinated pigs. Moderate amounts of PCV2 antigen were detected in most lymphoid tissues of nonvaccinated pigs. These data indicated that when given intramuscularly in pigs, the chimeric PCV1-2 live virus and recombinant plasmid induces protective immunity against PCV2 infection and could potentially serve as an effective vaccine.

攻毒后21天攻毒对照组猪淋巴结比免疫组猪显著肿大，免疫组猪血清、淋巴结中PCV2病毒载量低于对照组，攻毒对照组猪淋巴结中PCV2抗原含量高于免疫组，这些结果表明嵌合型猪圆环病毒PCV1-2及真核表达质粒肌肉注射免疫商品猪后，对PCV2感染能产生保护性免疫应答，有可能成为候选疫苗。

The viral particles were investigated by serial passaging. These following results were obtained.

采用感染传代实验研究嵌合病毒cDNA在人源化细胞中是否可以产生感染性病毒颗粒。

Objective: To study the effect of polycythemia on blood oxygen saturation.

裴蕾目的：观察RBC剧增而引起的高粘血症对血氧饱和度的影响。

Based on SIMPLER algorithm in the curvilinear body-fitted coordinates, the calculations were performed for Pr=0.7, Re=10~1000 on non-orthogonal non-staggered grids which are generated by elliptic equation systems.

采用曲线坐标系下压力与速度耦合的SIMPLER算法，数值研究了周期性渐扩渐缩波纹通道内脉动流动与换热情况，流动Re数的范围为10~1000，Pr数为0.7。