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The rats were divided into 6 groups and received chitosan embrocation, hyaluronidase injection, hyaluronidase injection plus chitosan embrocation, saline embrocation, or saline injection, or received no treatment as control.

随机分为6组,分别给局部外涂几丁糖、局部注射透明质酸酶、局部注射透明质酸酶联合外涂几丁糖、局部外涂生理盐水、局部注射生理盐水和不进行任何处理。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生;HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润,纤维细胞轻度增生,硬膜外神经根内有炎细胞浸润及炎性渗出;特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变;免疫组化染色,给药后四周及八周时,硬膜内外均有Ⅱ型胶原纤维增生;固兰染色B、C、D三组未见有明显脱髓鞘改变,与A组相比无明显异常改变;电镜观察B、C、D三组在给药后的四周及八周时,表现为硬膜外神经根内髓鞘厚薄不一,形状不规则,可见成纤维细胞,神经内膜中可见有巨噬细胞;粗大的有髓神经纤维变化明显,无髓神经纤维受累较轻;八周时电镜下D组改变较B、C两组为轻。

Methods Seventy-five SD rats were randomized into five groups: normal, model, iodine glycerin (20 g/L), high-dose SP (200 g/L) and low-dose SP (100 g/L). The experimental periodontitis rat models were established by ligating molars with steel wire combined with intramuscularly injection of prednisolone (1.25mg, 8 times). Except that the model and normal groups were treated by applying saline, the other groups were treated by applying the corresponding drugs 0.5 mL to the peridentium, tid for 10 days. After treatment, debris index, probe depth and bleeding rate during probing were investigated and the pathological changes of periodontal tissues were also observed.

方法]SD大鼠随机分为正常组,模型组,碘甘油组(20g/L),舒口散高、低剂量组(200、100 g/L);采用钢丝结扎牙齿加肌注波尼松龙(l.25 mg/次,共8次)的方法复制大鼠实验性牙周炎模型;药物采用口腔局部涂搽,每次0.5mL,每日3次,10 d为1疗程,模型组与正常组给予等容积生理盐水;检测各组软垢指数、探诊深度、探诊出血阳性例数,并取牙周组织进行病理学检查。

On the other hand, there were four cases of hematoma and three cases of pseudoaneurysm in preclose group. There was no statistal difference in terms of local complications between the 2 groups.

Angioseal组发生局部血肿5例,无假性动脉瘤发生;Perclose组发生局部血肿4例,假性动脉瘤3例,两组间并发症的发生率差异亦无统计学意义。

There were fewer positive cells in other groups. The change tendency from few to many is control 2, MP3→MP2, MP4→control 1, MP1 Conclusions 1. Methylprednisolone is a very potential anti-scar drug and can elevate successful rate of rabbit filtration. 2. Used in the way of cotton patch, MP has lower ocular toxicity. Samely, high concentration could be got topically. 3. In definite range, the activity of MP is concentration-dependent. In other words, the activity can be promoted with the increase of concentration. Total results considered, 100mg/ml MP group is identified to have the best effect of all MP groups in the experiment.

; 实3组与对2组脯间差异不显著(Pl.05),但与其它继间差异显著①<0.05);实2组和实4组间差异不显著,但与其它各组间差异显著(P<0.05人结论1、甲基强的松龙是—种很有潜力的低毒的摊痕抑制剂,可提高兔B湖过阶术的成功率;人甲基强的松溉用术中湿片贴敷的方式,既减轻了毒副作用,又可以达到局部用药的高浓度,是一种值得推荐的给药方法;3、在一定范围内,甲强龙的作用与浓度呈正相关关系,本次实验以 100ined组效果最好。

There were no significant differences for the firing rates in the site of contralateral TNC neurons among during pre-CSD,CSD,and post-CSD (P>0.05).For flunarizine group,the firing rates in the site of ipsilateral TNC neurons during pre-CSD were higher as compared with during CSD(P<0.05).2.1 There were statistical differences on palasma levels of CGRP and SP among the three groups(P<0.05).The levels of CGRP and SP in CSD group were higher than control group(P<0.05).No significant differences on the levels of CGRP and SP in ipsilateral trigeminal ganglia were found among the three groups(P>0.05).2 The number of neurons with positive CGRP and SP immunoreactivity was statistically different in right-sided trigeminal ganglia among the three groups (P<0.05).The number in fight-sided trigeminal ganglia in CSD group was higher as compared with control group(P<0.05).The number in right-sided trigeminal ganglia was statistically higher than that in left-sided trigeminal ganglion in CSD group(P<0.05).3.1 Altered ReHo in ipsilateral pons and other brain regions response to pain such as basal nuclei,thalamus,cingulated gyms and prefrontal cortex was detected during the acute spontaneous attack as compared with during headache remission(P<0.05,corrected by Monte Carlo simulation). 2 Positive functional connectivity was detected between ipsilateral pons and other brain regions related to pain within pain state and within non-pain state (P<0.05,corrected by false discovery rate,FDR).Increased functional correlation between ipsilateral pons and other pain-related brain regions such as ipsilateral prefrontal cortex and contralateral subcallosal gyrus was detected during the acute spontaneous attack as compared with during headache remission(P<0.05,corrected by Monte Carlo simulation).

结果1。对照组未发现CSD;同侧TNC放电频率,CSD中>CSD后>CSD前P<0.05对侧TNC放电频率,CSD前、中、后无统计学差异(P>0.05氟桂利嗪组同侧TNC放电频率,CSD前>CSD中(P<0.05),CSD前与CSD后及CSD中与CSD后之间无统计学差异(P>0.05)。2.1关于放免测定,各组血浆CGRP、SP水平有统计学差异(P<0.05),CSD组高于对照组(P<0.05),CSD组与氟桂利嗪组、对照组与氟桂利嗪组之间均无统计学差异P>0.05各组之间同侧三叉神经节中CGRP、SP水平未见变化(P>0.05.2关于免疫组化研究,右侧三叉神经节CGRP、SP免疫阳性细胞数三组之间有统计学差异(P<0.05),多重两两比较结果CSD组大于对照组(P<0.05),CSD组与氟桂利嗪组之间、对照组与氟桂利嗪组之间无统计学差异P>0.05左侧三叉神经节CGRP、SP免疫阳性细胞数三组之间无统计学差异(P>0.05CSD组中右侧三叉神经节CGRP、SP免疫反应阳性细胞数大于左侧(P<0.05)。3.1局部一致性分析发现两组患者头痛疼痛状态较非疼痛状态脑活动发生变化的脑区有同侧脑桥以及其他疼痛相关脑区如基底节区、丘脑、扣带回、前额叶皮层等(P<0.05,蒙特卡罗模拟校正)。2功能连接分析发现疼痛状态与非疼痛状态下主要疼痛相关脑区均与同侧脑桥有功能联系P<0.05,false discovery rate,FDR校正疼痛状态与非疼痛状态比较,同侧前额叶皮层、对侧胼胝下回等疼痛相关脑区与同侧脑桥之间功能联系增强(P<0.05,蒙特卡罗模拟校正。

The treatment group 61 examples cervix of the uterus partial used zhi kang capsul powder (Sanqi, ginseng, Chinese goldthread rhizome, hyacinth bletilla, so on), the control group 60 example cervixes of the uterus used the Yunnan medicinal powder partially,The treatment courses are 3~5d.for both growp.

治疗组61例宫颈局部使用致康胶囊药粉(三七、人参、黄连、白及、血竭等),对照组60例宫颈局部使用云南白药,两组疗程均为3~5d。

The right knee joint of rabbits were depilated and embrocated with Zhuang jin ye group or vehicle group once per day. There was no special treatment in the normal control and the model control group.

正常对照组及模型对照组不作处理,壮筋涂膜液组将壮筋涂膜液涂布于已脱毛的患膝局部,每日一次,赋形剂组将不含药的涂膜液基质涂布于已脱毛的患膝局部,每日一次。

To observe the curative effect of blood-letting puncture on acute soft tissue injury.42 cases were percussed heavily by pyonex and fast inserted by triangular-edged needle for bleeding.The visual simula- tion and evaluation standard of curative effect were performed.The results showed that the joint pain had greatly relieved,and other symptoms had obviously improved,the total effective rate was 93.02%.

采用局部梅花针重叩、三棱针点刺出血的方法,观察刺络治疗急性软组织损伤42例,与局部针灸治疗对比,应用视觉模拟法及疗效判定标准,比较2组治疗后的症状改善情况,结果:刺络治疗组关节疼痛明显减轻,症状明显改善,总有效率93.02%。

RESULTS: Compared with acupuncture and Chinese herb used separately, acupuncture and herb combination significantly decreased the level of reduced viscosity in whole blood, plasma viscosity, blood sedimentation and erythroagglutination index..Significant increases of TMPH in the heart, liver and hind paw tissue except kidney were observed after the combined treatment, which also raise cAMP level in heart and liver.

结果:针药组大鼠的全血还原粘度、血浆粘度、血沉、红细胞聚集指数均较单纯针刺组、单纯药物组显著降低;单位时间内针药组大鼠体内(心脏、肝脏、病灶局部组织)盐酸川芎嗪含量明显高于单纯药物组,肾脏中却无明显增高;同时针药组大鼠心脏、肝脏中的cAMP含量明显高于单纯针刺组和单纯药物组。

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