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Methods40 adult male SD rats were used in this study and divided into 4 groups at random: normal group, aging model group, TNY treatment group, and negative control group, each group had 10 rats. The subacutely aging rats were made by injecting Dgal into the rats' abdominal cavity continuosly. The learning and memory ability of rats in each group were detected with Morris water maze. The monoamine transmitter contents in hypothalamus and hippocampus were detected with HPLCECD.

方法选用成年雄性SD大鼠40只,随机分为4组,每组10只:正常组、衰老模型组、TNY用药组、阴性对照组。D半乳糖连续腹腔注射制作亚急性衰老的大鼠模型,采用Morris水迷宫检测各组大鼠的空间学习记忆能力及采用高效液相色谱电化学法检测各组大鼠下丘脑、海马NE,DA,5HT的含量。

In RD group, subretinal injection with 10 mg/ml hyaluronic acid sodium was performed on the left eyes of the rats to set up RD model, and the left eyes and retinal tissue were collected 1/2 day, 1 day, 2, 4, 8, 16 and 32 days after RD; there were 11 rats in each subgroup.

方法88只Wistar大鼠分为2组:正常对照组11只鼠;RD组77只鼠,左眼视网膜下注射10mg/ml透明质酸钠建立RD模型,分别在RD后1/2、1、2、4、8、16、32d收集大鼠左眼球及视网膜组织,RD后各时间组每组各11只大鼠

In RD group, subretinal injection with 10 mg/ml hyaluronic acid sodium was performed on the left eyes of the rats t o set up RD model, and the left eyes and retinal tissue were collected 1/2 day, 1 day, 2, 4, 8, 1 6 and 32 days after RD; there were 11 rats in each subgroup.

88只Wistar大鼠分为2组:正常对照组11只鼠;RD组77只鼠,左眼视网膜下注射1 0 mg/ml透明质酸钠建立RD 模型,分别在RD后1/2、1、2、4、8、16、32 d收集大鼠左眼球及视网膜组织,RD后各时间组每组各11只大鼠

METHODS: Two groups of rats [allograft group and syngenic graft group] underwent limbal transplantation and healthy rats were taken as normal controls. The expression of RANTES and MCP 1 mRNA was detected by quantitative competitive RT PCR at post operative days 1, 3, 7, 11, 14, 21 and 30 and rejections were observed.

大鼠随机分为同种异体移植组、自体移植组,并以健康大鼠作为正常对照组,用定量竞争性RT PCR的方法,在术后1, 4, 7, 11, 14, 21和30 d分别检测各组大鼠RANTES和MCP 1 mRNA的含量,并观察大鼠眼表变化,判断排斥反应的发生。

Methods: Fifty-four female SD rats (8-month-old) were randomly divided into three groups, 18 rats in each group, They included a sham-operated group, an ovariectomized group and a tonifying kidney recipes therapy group, After 3 months, the animal models of osteoporosis were established, pour titanium smooth columnar implants were placed in the proximal metaphyses of left tibiae of the rats.

选用纯种8月龄雌性SD大鼠54只,随机分为3组:假手术组,卵巢切除组,卵巢切除加中药治疗组,每组18只。切除卵巢切除组及中药组大鼠双侧卵巢,假手术组大鼠仅切除双侧卵巢附近的脂肪组织。术后3个月,在三组大鼠胫骨近中干骺端分别植入纯钛光滑柱状种植体。

Intra-portal alopecia was observed in all rats which received 30 Gy and some rats which received 20 Gy. The abnormal neurological signs were not found in all the rats, but the tend of body weight increase was less pronounced in 1-3 months in the irradiated rats than those unirradiated.

所有存活大鼠无异常神经系统体征,30Gy剂量组全部和20Gy部分大鼠照射后射野内有脱毛,而且照射组大鼠在受照后1至3个月期间,体重的上升趋势低于未照射组大鼠,但无统计学差别。

ResultsThere was no expression of Caspase-3 in group A. The expression of Caspase-3 in group C was decreased compared with that in group B(P<0.01. In groups B and C, expansion and vacuolization of mitochondria, swollen neurons and apoptotic bodies appeared, which suggested that WBH could induce neuron injury, and the neuron injury in group C was more severe than that in group B.

结果A组大鼠海马区神经元无Caspase-3表达,B、C两组大鼠海马区神经元有Caspase-3表达,B组较C组Caspase-3表达平均灰度值减小。B、C两组大鼠海马神经元超微结构发生改变、细胞器水肿,部分线粒体空化,内质网稀疏,突触前膜、后膜结构不完整,可见自噬体结构;B组大鼠的神经元超微结构损害较C组轻。

(1) Oxidative/antioxidative reactions, inflammatory reactions, apoptosis in lung are involved in the pathogenesis of HILI;(2) ABX, rHuEPO have protective effects on HILI respectively;(3) Hyperoxia can increase VILI in rats, but has little effects with low tidal volume mechanical ventilation.

结论(1)氧化抗氧化失衡,炎症反应,肺内细胞凋亡参与了高氧所致成年大鼠肺损伤的过程;(2)ABX通过抗炎、抗氧化、抑制细胞凋亡作用对成年大鼠高氧肺损伤具有保护作用;rHuEPO通过抗炎、抗氧化、抑制细胞凋亡及一定程度的抗纤维化作用对成年大鼠高氧肺损伤具有保护作用;(3)高浓度氧能明显加重大鼠通气机所致肺损伤,而高浓度氧对小潮气量通气影响较小。

Methods: The model of acute myocardial ischemia in rats was developed by ligaturing the left descending anterior coronary artery. Forty male Wister rats were divided into normal control group, ischemia model group, Xuesetong group and Verapamil group. The effect of Xuesetong on the CPK, LDH, SOD and MDA in serum and myocardium and myocardial infarct range was observed.

采用结扎大鼠冠状动脉左前降支复制出大鼠心肌缺血再灌注损伤模型。40只Wister大鼠分为正常对照组、模型组、血塞通组及维拉帕米组,观察血塞通对大鼠血清磷酸肌酸激酶、乳酸脱氢酶、血清及心肌组织超氧化物歧化酶和脂质过氧化物丙二醛及对心肌梗死范围的影响。

To approach its" mechanism of action, we carded out this experiment. The SD rats were objects in this experiment. We made rat focal cerebral ischemia model with middle cerebral artery occlusion using a thread block technique. We gave a mark of the neurologic impairment in rat and detected the contents of calcitonin gene-related peptide and neuropeptide Y in rats" cornu ammonis and brain mantle respectively by electro-acupuncture on shuigou(DU 26) acupoint. In this way, we are trying to explore the mechanism of acupuncture therapy in cerebral ischemia.

为了进一步探讨其作用机理,本实验以SD大鼠为研究对象,采用线栓法制作大鼠局灶性脑缺血模型,运用电针水沟进行治疗,并对大鼠神经功能缺损进行评分,检测了大鼠海马及大脑皮质降钙素基因相关肽以及神经肽Y含量,从中枢神经肽类递质的变化探讨电针抗脑缺血损伤的作用机理,为临床治疗缺血性脑血管疾病提供实验依据。

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