复制子

Further studies also show that Dicer can inhibit the replication of the HCV subgenomic replicon.

进一步的研究显示Dicer能抑制HCV的亚基因复制子的复制。

WORD PROCESSOR Spell Checker (20+ langs., check block/all), Thesaurus, full control of fonts, colors, highlight color, left/right/center/justify/indent text, page margins, bullets, table color/shading/border/resize, format painter, text-sort, block-move, line/word-select, char.map, 100-level undo/redo, second editor pane etc. ATTACHMENTS: include files of any type and reasonable size; TREE FEATURES copy/paste, expand/collapse,sort, number tree, order tree-nodes through drag-drop, copy subtree directly to another database.

字处理器的拼写检查（ 20 + langs ，检查座/所有），词库，完全控制字体，颜色，突出的颜色，左/右/中心/理由/缩进文字，页边距，子弹，表颜色/阴影/边界/大小，格式的画家，文本排序，分块移动，线/字选择， char.map ， 100级别的撤销/重做，第二编辑窗格等附件：包括文件的任何类型的和合理的规模；树的特点复制/粘贴，展开/折叠，排序，有多少树，为了树节点通过拖放，复制子树直接向另一个数据库。

The multi-copy expression vector pZM is a shuttle plasmid which can replicate in Escherichia coli, Streptomyces and S. erythraea. It contains PermE promoter, fd terminator, multiple clone site, thiostrepton and ampicillin resistance genes, and ColE1 ori and pJV1 ori replicons which make the vector replicate in E.

游离型多拷贝表达载体pZM是可以在大肠杆菌、链霉菌和糖多孢红霉菌中扩增的穿梭型质粒，带有PermE启动子、fd终止子、多克隆位点、硫链丝菌肽和氨苄青霉素抗性基因以及在大肠杆菌和糖多孢红霉菌中复制的ColE1 ori和pJV1 ori复制子，载体大小9880bp。

Methods 1.3 fold overlength genome of HBV,from pHBV-dimer carring two head to tail copies of HBV genome,was cloned into pCR2.1 by combined PCR and restriction enzyme digestion.

目的 构建乙型肝炎病毒感染性复制子表达载体，建立HBV体外复制细胞模型，分析其复制特性及对抗病毒药物的敏感性。

Several fragments containing the replication origin were cloned into replicon probe vector pUC-MEV constructed in this study. After screening a 4. 8kb PvuⅡ-SnaBⅠ fragment were determined to be the minimum replication region of the plasmid.

将包含复制起始原点在内的数个片段分别插入构建的嗜盐古生菌的复制子探针载体pUC-MEV中，通过筛选得到了pHH205最小复制功能片段。

The approach usually used to distinguish individual replicons from fused eyes is to rely on stretches of DNA in which several replicons can be seen to be active, presumably captured at a stage when all have initiated around the same time, but before the forks of adjacent units have met.

区分独立复制子与熔化眼的常用方法是依靠DNA的伸直，因为在其中几个复制子能够发现具有活力，假设当所有的复制子在相同时间内已经起始时在同一状态时被观察，但必须在连接单位的复制叉相遇之前。

Then the HCV subgenomic replicon cell model was incubated with Napp at concentration of 1mg/ml, 10~(-1)mg/ml, 10~(-2)mg/ml, 10~(-3)mg/ml, 10~(-4)mg/ml in cell culture solution and the negative and positive controls were set up correspondingly. After 3 days, the intracellular RNA of HCV subgenomic replicons were detected by FQ-RT PCR.

然后分别取终浓度为1mg/ml、10~(-1)mg/ml、10~(-2)mg/ml、10~(-3)mg/ml、10~(-4)mg/ml的Napp与含HCV亚基因复制子细胞培养液中共同孵育，同时设空白对照组和IFN-α2b阳性对照组，3天后用荧光定量PCR法检测HCV复制子中RNA的含量。

To rely on stretches of DNA in which several replicons can be seen to be active, presumably captured at a stage when all have initiated around the same time, but before the forks of adjacent units have met.

区分独立复制子与熔化眼的常用方法是依靠DNA的伸直，因为在其中几个复制子能够发现具有活力，假设当所有的复制子在相同时间内已经起始时在同一状态时被观察，但必须在连接单位的复制叉相遇之前。

It has been shown that the replicon has no significant impairment on the growth and metabolism of host cells.

复制子在人肝癌细胞株huh 7细胞株中能高水平的自主复制。

Objective To construct expression vector of HBV infectious replicon and establish hepatitis B virus replication model in vitro, and observe the dynamic changes of viral replication and its sensitivity to antiviral durg.

目的 构建乙型肝炎病毒感染性复制子表达载体，建立HBV体外复制细胞模型，分析其复制特性及对抗病毒药物的敏感性。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。