增序列

The gene regulating sequence of human collagen type Ⅰ is located at 5'end and first intron. The carriers of reporting gene was made, and the function of promoter, enhancer and inhibiter was examined in this study. On the basis of above work, MBP ectopic expression carriers were further built. The result laid a foundation for investigating the feasibility, and validity to specially express MBP gene of human nervous system, in tendon fibroblast.

人Ⅰ型胶原基因调节序列位于5'端和第一内含子，本研究构建了带报告基因的载体并检测了其启动子，增强子和减弱子的功能，以此为基础，进一步构建了MBP异位表达载体，为探索在神经系统少突胶质细胞特异表达的人脑髓鞘碱性蛋白MBP基因转至肌腱成纤维细胞表达的可行性和有效性奠定了基础。

Methods:The expressive plasmids of AML1b and AML1-ETO were transfected into CV-1 and 293 cells.The expression level of endogenous pig7 gene was detected by Realtime-PCR.The luciferase reporter plasmids containing pig7 enhancer/corresponding mutant sequences were constructed and co-transfected into CV-1 cells with expressive plasmids of AML1b and AML1-ETO.The transactivity of pig7 enhancer was assayed by luminometer.

研究方法：AML1b和AML7-ETO表达质粒分别转染及共转染CV-1和293细胞，用Realtime-PCR方法检测上述基因对两种细胞内源性pig7基因mRNA表达水平的影响；构建含AML1b结合位点的pig7基因增强子序列以及相对应的突变位点的荧光素酶报告基因质粒；将表达载体/突变载体与AML1b或AML1-ETO基因瞬时共转染CV-1细胞，分析AML1b、AML1-ETO对报告基因的转录调节作用。

A discrete weighted Hardy inequality is studied, and by proving a refinement lemma, the characterization of the weight sequence ｛ω_｝_(n≥1) in order that the weighted l~-Hardy inequality holds for all nonnegative and nonincreasing sequence c_ is given.

本文研究离散的加权Hardy不等式，通过建立权序列的加细引理，给出了对任何非负非增数列 lp-加权Hardy不等式成立时权序列{ωn}n≥1的特征刻划。

We consider Theorem 1 to be highly significant and apply it to obtaining the rank of a certain augmentation quotient group by proposing Theorem 3 and giving its complete proof.

应用具有Np-序列有限p-群的特殊性质和重量函数，基本序列等概念以及已有的一些结果，分别研究了类为1的pk(k 2)阶A bel基本p-群和类为2的p4阶基本p-群之增广商群Qn的结构，得到了当n足够大时Qn作为A bel基本p-群的秩。

Firstly, it is found that there are many obvious differences between Acorus and Araceae. Their differences are based on many aspects which include characteristics of morphology, anatomy and epidermis of leaf, types of tapetum in anther walls , patterns of endothecial thickenings, and ways of development of endosperms, presence or absence of perisperm, components of photochemistry, and sequences of rbcL.

研究表明：1。菖蒲属与天南星科其它属在叶的形态、结构、表面特征，花药绒毡层类型，药室内壁增厚的特点，胚乳的发育方式，外胚乳的有无，植物化学成分，rbcL基因序列等多方面存在着显著的差异。

According to the classical signal transduction pathway of estrogen, we use Luciferase reporter gene to screen phytoestrogen form Selaginella tamariscina, Pinus massoniana Lamb, Corallodiscus flabellate, Dryopteris sublaeta Ching et Hsu and Leonurus heterophyllus. In this study,the pβgal-Control vector, the pERE-TAL-luc vector, the pCNX2-hERα/pCNX2-hERβvector were transfected into HEK293 cell line. If the components in the herbs combine with the ER, then it could induce the expression of reporter gene controlled by ERE.

根据体内的雌激素信号转导途径，我们选用了以luc为报告基因、ERE为调控序列的重组报告基因载体pERE-TAL-luc，并将其与重组人ERα(humanERα，hERα)表达载体pCXN2-hERα和重组人ERβ(humanERβ，hERβ)表达载体pCXN2-hERβ共转染ER阴性细胞系，建立了靶向ERα或ERβ的细胞水平药物筛选模型，结合小鼠子宫增重实验，对卷柏、松针、益母草、石胆草及贯众等5种中药的植物雌激素作用进行了研究，以期为临床防治更年期综合症等提供准确、科学的实验基础。

In the last part of thesis, primary study of enhancer binding protein was done using yeast one-hybrid system.

在本文的最后一部分，首次应用酵母单杂交系统对痘苗病毒增强子序列在大肠杆菌中的DNA结合蛋白作了初步研究。

It should be pointed out that designing an efficient solution method for a nondifferentiable optimization problem is not an easy task.

作为统一的算法方面的研究，我们可以看到增广的拉格朗日方法可以用来解上述的第一类问题，而基于灵敏度的分析的序列二次规划方法完全有能力解上述的第二类问题。

Erve growth factor combined with its receptor, activated Ras - MAP kinase , mitogen - activated protein kinase kinase is the important regulating factor that make IkB kinase, IKK, phosphated, IkB kinase make the IκBα:(the subunit of NF -κB ) phosphated, the phosphated IkBα degraded, and p65 - p50 heterodimer can be formed, then the heterodimer translocated to nucleus and combined with the promoter domain or other consensus sequence.

GF与其受体相结合，最后可以激活Ras-有丝分裂激活的蛋白激酶途径，有丝分裂激活的蛋白酶激酶(MEKK1)是IγB激酶发生磷酸化的重要调节因子，IKK使NF-κB的亚单位IKBα发生磷酸化，磷酸化的IKBα发生降解，而最后留下p65-p50二聚体，该二聚体然后转位到细胞核内与κB基因的增强子区域或与他的顺式作用序列结合。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克，果实色泽鲜艳，汁多味浓，甜酸适口，离核仁甜，清末时就驰名陕、甘两省，清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。