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The results showed that coniferin could completely polymerize with model compound of β-O-4 type lignin to form lignin macromolecule. The M of the polymerized product was much higher than the starting materials.

研究表明:在漆酶的催化作用下木质素前驱物松柏醇-β-D-葡萄糖苷与木质素模型物能很完全的发生聚合,产物中不存在起始物的晶体,M也大大的增加了。

Using a comparative and simulative approach, this study quantitatively explored the effects of macrobenthos (Ilyoplax deschampsin and Corbicula fluminea) on the exchange of nitrogen across the sediment-water interface and the early diagenesis of nitrogen in tidal sediments from the Yangtze Estuary....

研究表明,谭氏泥蟹主要通过掘穴活动增加沉积物-水-气三相接触界面,促进沉积物中的无机氮(NH+ 4- N、NO- 2 -N和NO- 3- N)向上覆水体中扩散,并且也加快了沉积物中氮的氨化作用和硝化作用速率,而河蚬则主要通过生理活动机制影响潮滩生态系统内氮素的迁移转化过程。

Corbicula fluminea however affected the exchange of nutrient nitrogen across the sediment-water interface by the physiologic mechanism such as the excretion. Crabs such as Ilyoplax deschampsin changed the physical structure of the tidal flat sediments by the strong bioturbation, and increased the content of dissolved oxygen in the sediment by enhancing the touched area of sediment and air.

谭氏泥蟹主要通过生物扰动,增加沉积物-水-气三相界面之间的接触面积,促进沉积物界面附近营养盐物质交换过程;而软体动物河蚬则主要通过生理活动,影响潮滩沉积物中氮营养盐的界面扩散过程。

Since the dismutation of superoxide radicals by SOD is a reaction that is rate-limited by diffusion and enhanced by electrostatic guidance, all these structural features may be related to GP-MnSOD's high catalytic activity: first, Lys38 may increase the effective enzyme-substrate association rate by enhancing the electrostatic gradient at its substrate channel; second, an excess of acidic over basic residues makes the negative surface potential of GP-MnSOD more larger than those of other known MnSODs. This may enhance the reaction rate by reducing futile collisions of superoxide radicals with GP-MnSOD's surface.

因此,GP-MnSOD的上述结构特征可能与GP-MnSOD的较高酶活有关:首先,Lys38增加了底物通道口的正静电势梯度,从而可能促进了GP-MnSOD的活性中心与底物的有效结合速率;其次,GP-MnSOD表面覆盖的过量酸性残基使得它比其它MnSOD在底物通道以外的蛋白表面更多地被负静电势所覆盖,这可能有效地减少了超氧阴离子与GP-MnSOD表面的无效碰撞,从而提高了反应速率。

Increasing the surface of plant fiber and the ratio of activated sludge to plant fiber can improve the degradation degree of plant fiber.

增加降解物的表面积和提高活性污泥与降解物的比值,有利于降解物的降解。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实:两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用,并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著,最佳效应浓度为400nM;2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响:①CREG蛋白对VSMC迁移的影响:刮伤实验发现,加入最佳效应浓度的wtCREG和mCREG蛋白24h后,OB2组迁移能力下降,HITASY组无明显变化;细胞外基质金属蛋白酶-2,9(Matrix metallo-proteinase 2,9,MMP2 ,9)明胶酶电泳检测和Western blot检测结果证实,两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2,9减少,而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases,TIMPs)增加;②CREG蛋白对VSMC分化的影响:加入400nM的wtCREG和mCREG蛋白12h后,OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加,LM-1、FN表达减少;③流式细胞仪分析细胞周期和BrDU染色分析证实,加入400nM的wtCREG和mCREG蛋白后,OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572,HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034;3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用:①免疫共沉淀和免疫荧光双染色分析结果显示,CREG蛋白与M6P/IGF2R存在直接结合;②应用抗体阻断实验:将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中,CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱,而且与加入anti- M6P/IGF2R浓度正相关。

For the protein side chain,the band at 1 368 cm-1 assigned to the "buried" tryptophan shifted to 1 365 cm-1 of the "exposed" and the relative intensity reduced 59%. The relative intensity of 635 cm-1 assigned to the gauche conformation of the C—S decrased 15% and the band at 725 cm-1 increased 58%.These indicate that the structure of the protein changed in the diabetic serum.The relative intensity at 1 449 cm-1 assigned to the lipids characteristic increased 58%.The relative intensity of the glucide characteristic at 1 331,1 099 and 740 cm-1 increased 35%,100% and 62%,respectively.

蛋白质侧链色氨酸吲哚环&埋藏式&的谱线1 368 cm-1位移到&暴露式&的1 365 cm-1,且相对强度减少了59%;侧链C—S键的扭曲构象的谱线635 cm-1相对强度减少了15%,而反式构象的谱线725 cm-1增加了58%,说明C—S键的扭曲构象部分转变为反式构象,表明糖尿病人血清中蛋白质的有序结构发生变化;类脂物特征峰1 449 cm-1相对强度增加了58%,与糖类有关的特征峰谱线1 331,1 099和740 cm-1相对强度分别增加了35%,100%和62%,从而表明糖尿病人血清中与脂类和糖蛋白有关的物质含量增加。

As compared with the traditional irrigation-traditional fertilization, the following results of the improved optimized irrigation-optimized fertilization were obtained: significantly higher nitrogen use efficiency; the like tiller number per unit area, percentage of the tiller ear number/the tiller number, the ear number per unit area, the greatly reduced grain number per ear, and significantly heavier weight of 1, 000 grains; a trifle taller plants; shorter base internode length, a bit more weight per unit internode length, longer internode length under ear; almost the same LAI values in middle and later stages; coincident process of dry matter accumulation from straight growth onwards, a little more dry matter accumulation amount from earing to maturity; a little longer flag leaf, 2〓 leaf, 3〓 leaf (but the three leaves were shorter than those of the optimized irrigation-traditional fertilization) and shorter 4〓 leaf from top, a bit reduced total area of these leaves; almost the same leaf thickness, mean canopy leaf tilt angle, light extinction coefficient , ratio of light penetration in the upper, middle and lower parts of the wheat canopy, and fair plant type; almost the same senescence process of flag leaf; and increased root amount in 0-120cm soil layer and smaller rate of progressive decrease of the root length density and root weight density with soil getting deep, which can strengthen the resistance to adverse conditions such as water stress in the later stage.

在优化水肥条件下,冬小麦总分蘖数、成穗率及单位面积穗数与传统水肥比较接近,但穗粒数显著减少,千粒重显著提高;株高稍有增加;基部节间长度缩短,充实度略微增高,穗下节间增长;中后期LAI与传统水肥的LAI值基本吻合;起身至成熟干物质积累量动态变化过程与传统水肥十分接近,但抽穗至成熟所形成的干物质量增加;上三叶变长(但上三叶长度增加幅度小于优化灌溉-传统施肥处理),倒4叶变短,总叶面积略有减少;叶片厚度、冠层平均叶倾角、消光系数、冠层各层的透光率与传统水肥差异很小,株型较好;旗叶叶绿素含量动态变化与传统水肥十分接近;0-120cm土层的总根量明显增加,上层根群和深层根群的差异最小,有利于提高小麦后期的抗逆力。

With the increase of the deforested years, soil erodibility parameter increases.

2随着植被破坏开垦年限的增加,土壤容重逐渐增大,而毛管孔隙度逐渐减少;土壤水稳性团聚体呈减少趋势,且小团聚体所占比例逐渐增加;物理性粘粒随开垦年限的增加而减少,而粗粉砂呈增加趋势,表明土壤粗化现象明显。

The molecular motion of amorphous region at high-frequency was found to be hardly affected during the stretching processes. In summary, elongation has created major but different influences on the morphology and molecular motion of the aforementioned four series of polymeric elastomers. For those elastomers like natural rubber and poly , elongation has induced crystallization of the noncrystalline or the soft segments. The crytallinitiy and the crystal thicknesses increase monotonously during the course of the stretching; On the other hand, the stretching processes for those semicrytalline copolymers like EMA and POE are rather complex, including the formation of crystal at relatively low λand the destruction of the crystal at the utmost λ. It revealed that stretching process has resulted in a decreasing of the thicknesses and a shortening of 〓C T〓s for the orthorhombic crystalline components.

拉伸对上述四个弹性体体系的聚集态结构都产生了很大影响,但是影响的程度和内容因体系而不同,对于本身存在着化学交联或者是物理交联的体系而言,拉伸导致了其中本来难以结晶组分的结晶,且结晶度随着拉伸比增加而增加,与此同时,体系结晶的晶片厚度也会逐渐增加;而对于结晶性共聚物体系,如EMA和POE,结晶区本来起到了物理交联的作用,在这种情况下拉伸的影响变得比较复杂,拉伸对于不同晶型的影响有所不同,在拉伸比较低的情况下体系总体的结晶度会有所增加,说明有新的结晶产生,当接近或达到断裂伸长比时,体系的结晶度会突然降低,含量占优的结晶会被拉伸所破坏,含量较低的结晶在整个拉伸过程中受的影响较小。

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