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The type, initial feeding time and concentration of precursor for HV unit formation had great effects on PHBV production in shake flasks. Propionate was selected as the better precursor for HV fraction than valerate after comprehensive survey of these two precursors. The suitable PHBV shaking-flask culture conditions were obtained through the analysis of PHBV fermentation process, the investigation of several factors by using RSA method and the fed-batch experiments. Cell dry weight, PHBV content, PHBV concentration, HV fraction and yield coefficient of HV unit to propionate were 27. 7g/L, 80.7%, 22. 4g/L, 16. 1mol% and 0.50g/g, respectively.

在共聚物PHBV的摇瓶发酵过程中,PHBV中HV组分形成的前体物质的种类、初始加入时间以及加入量是影响PHBV发酵过程的重要因素;在综合比较丙酸和戊酸这两种前体的基础上,选择丙酸作为HV组分合成前体较适宜;通过对PHBV发酵过程曲线分析、影响PHBV发酵指标的各因素的响应面分析以及摇瓶发酵补料试验,得到了较适宜的PHBV摇瓶发酵补料条件,在此条件下,细胞干重、PHBV含量、PHBV浓度、HV组分含量和HV组分对丙酸的产率系数可分别达到27.7g/L、80.7%、22.4g/L、16.1mo1%和

The results show that a little amount of heteropolymolybdovanadophosphate can remarkably speed the degradation, the best conditions of the oxidative degradation of chitosan with hydrogen peroxide catalized by heteropolymolybdovanadophosphate in homogeneous condition are as follows: the ratio of catalyst and chitosan is 1 to 10, concentration of hydrogen peroxide is 0.15%, the temperature is 80℃, reaction time is 2h. 0.2g chitosan was degraded under the best condition and the molecular weight by viscometry was degraded from 1460000 to 7812. then water-soluble chitosan was obtained by this method; The heterogeneous method is a high efficient way, the best condition of this method are as follows: the ratio of catalyst and chitosan is 2 to 100, the volume of 30% hydrogen peroxide is about 3mL, the temperature is 90℃, reaction time is 5min~30min.

研究结果表明,适量磷钼钒的存在可显著加速壳聚糖的降解,磷钼钒催化过氧化氢均相氧化降解壳聚糖的适宜反应条件是:催化剂与壳聚糖的质量比为1∶10、过氧化氢百分浓度为0.15%、反应温度为80℃、反应时间为2h,在此条件下对0.2g溶解在100 mL 0.5%稀醋酸中的壳聚糖进行降解,可使壳聚糖的粘均分子量由原来的146万下降到七千多(7812),成为水溶性低分子量壳聚糖,从而达到应用的要求;在异相条件下以磷钼钒为催化剂催化过氧化氢氧化降解壳聚糖是一种高效快速的方法,此法降解壳聚糖的最佳反应条件是:催化剂与壳聚糖的质量比为2∶100;30%过氧化氢的用量为3mL左右;反应温度为90℃、反应时间为5min~30min。

This article mainly discusses the large-scaled cultural conditions of clone in Gametophyte Laminaria .It studies the proper temperature, light time, light intensity, the consumption of nitrogen and phosphorus, the cultural state and inoculating density on the growth of gametophytic clone, the experiment of large-scaled culture had been done under comparatively proper conditions, in the same time, we studied the growth rule of gametophytic clone in the cultural process.

本文主要探讨了海带配子体克隆的大规模培养条件,对海带配子体克隆生长的适宜温度、光照时间、光照强度、营养盐消耗、培养状态以及接种密度进行了研究,并在相对适宜条件下进行了大规模培养实验,同时,对海带配子体克隆在培养过程中的生长规律进行了研究。

Results show the optimal conditions for the moringa seed oil extraction are as follows: using petroleum ether as solvent,the ratio of solvent to moringa seeds is 8∶1,extraction time 40 min and extracting twice with the aid of 28 kHz ultrasonic.At the conditions,the ...

结果显示,超声波辅助溶剂萃取辣木籽油的最佳条件为:溶剂石油醚,提取次数2次,提取时间40 min,超声波频率28 kHz,液料比8∶1,在此条件下,辣木籽的平均出油率为35.85%,提取率96%,表明超声波辅助溶剂萃取法是一种可用于萃取辣木籽油的适宜方法。

The redox separation conditions were investigated in detail. It was indicated that TBAPI exhibited appropriate oxidation intensity and well selectivity, thiolethers can be oxidized into sulphoxide, but it cannot oxidize thiols.

详细考察了氧化还原分离条件,实验结果表明,TBAPI具有适宜的氧化强度和良好的选择性,它能将硫醚硫氧化成亚砜,且在同条件下,对噻吩硫不起作用。

The experiment results showed the characteristics of the fermental Toxigenic Pasteurella multocida could meet the standard of producing AR vaccine in the colony morphology, toxin pathogenesis, and other aspects. The serotype of fermental Toxigenic Pasteurella multocida did not change.

实验结果表明,在适宜条件下D型产毒素Pm13-1株培养物符合其菌落、菌体形态特征;糖发酵实验结果符合其生化特性;其产毒素能力符合对制苗菌株产毒素、致病变能力的要求,且未发生血清型的变化。

Under optimal condition, the nitrifying bacteria performed well in NH4(superscript +)-N removal.

在适宜条件下,海洋硝化细菌对海水中氨氮具有较好的去除效果。

The stems of bamboo,a tropical grass, can grow at the phenomenal rate of 0.3m/day under optimal conditions.

热带草本植物竹子的茎,在适宜条件下,可以惊人的速度(0.3m/每天)生长。

Transformation yield was observed for the monolayers.

在适宜条件下我们观察到14%的光电转换效率。

Based on an experiment for the adsorption of trace silver by xanthic ester cotton under different conditions,the most suitable condition for such adsorption has been determined.

本文通过黄原酯棉对痕量银在不同条件下的吸附试验,找出黄原酯棉对痕量银吸附的最适宜条件,为在分析化学以及环保中利用黄原酯棉分析痕量银提供参考。

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Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

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