叶脉

Sazanami's features are the green veins on yellowish and lancerated spring lea ves.

Sazanami的特色就是春天在淡黄色叶片上的绿色叶脉。

The mutant displays defects in both compound leaf architecture and leaflets polarity pattern. Backcross test indicates the nl1 phenotype is caused by mutation on a single recessive locus.

研究显示nll突变表型是由单位点隐性突变造成的，突变体中小叶原基提前起始、小叶极性模式改变、叶脉模式改变，同时影响花器官发育和茎维管束模式建成。

The mutant displays defects in both compound leaf architecture andleaflets polarity pattern. Backcross test indicates the nl1 phenotype is caused by mutation ona single recessive locus.

研究显示nl1突变表型是由单位点隐性突变造成的，突变体中小叶原基提前起始、小叶极性模式改变、叶脉模式改变，同时影响花器官发育和茎维管束模式建成。

Nigra during the experiment. Successfully established the transformation procedure Agrobacterium-mediated of Populus simonii×P nigra: cut vigorous leaves to 2-3 patches through the main vein after growing about 20 days, pre-culture time was 3~4days;centrifuge Agrobacterium when being developped to the logarithm growth timeOD_(600=0.5, then dilute Agrobacterium to the concentration of OD_(600)=0.3-0.4 with MS liquid culture medium or asepsis distilled water for infection;infection time was 6-15 minute;after being co-cultured 3 days, inoculate on the selecting medium to be filtrated in succession during inducing unsureness buds and rooting with Kanamycin(containing Kanamycin 40mg/L and Cefazolin Sodium 700mg/L).

成功摸索出农杆菌介导的小黑杨遗传转化体系：选取生长20d左右的健壮的小黑杨组培苗叶片，通过主叶脉剪2～3下，预培养3～4d；将培养至对数生长期的农杆菌OD_(600=0.5离心后用液体MS培养基或无菌蒸馏水稀释成OD_(600)=0.3～0.4浓度，进行浸染；侵染时间为6～15min；共培养3d后，转入到筛选培养基中进行诱导不定芽及生根阶段的卡那霉素连续筛选(含有40mg/L卡那霉素和700mg/L的头孢唑林钠)。

What are your goals and strategies for growth?

你的成长目标和策略是什么？

And unto the angel of the church in Sardis write; These things saith he that hath the seven irits of God, and the seven star I know thy works, that thou hast a name that thou livest, and art dead.

3：1 你要写信给撒狄教会的使者，说，那有神的七灵和七星的，说，我知道你的行为，按名你是活的，其实是死的。