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After analyzing glutinous rice waxy gene sequence reported by previous studies, two pairs of dominant and codominant STS molecular markers based PCR were designed for distinguishing glutinous rice, according to the distinctions between glutinous rice wx gene and nonglutinous rice Wx gene. Moreover, the whole sequences of the two Wx genes of nonglutinous rice reported by others were also aligned, and another two pairs of dominant STS molecular markers were designed to discriminate Wxa from Wxb based on the special site between the two genotypes. Then, corresponding rice genotypes were identified by the established new markers.

通过对前人公布的糯性水稻蜡质基因全序列测定结果的分析,根据糯性水稻与非糯性水稻蜡质基因序列位点的差异,设计了两个基于PCR扩增反应的可特异识别糯性水稻的显性和共显性STS分子标记;同时在前人公布的非糯性水稻蜡质基因的不同类型的全序列比对分析的基础上,选取了两种基因型特定的差异位点,设计了两个基于PCR扩增反应用于特异识别这两种Wx基因的显性STS分子标记,并用新建立的标记对相应的水稻基因型进行了检测。

The conclusions were as follows: theνO-H andνC=O IR absorption maxima shifted towards higher wave numbers after imprinting 17β-estradiol on TFMAA-co-TRIM copolymer with the red shift of theνO-H groups being apparent after precipitation polymerization for 16h while that forνC=O groups being observed after polymerization for 24h. A strong interaction between TFMAA and 17β-estradiol was confirmed by the high selectivity for 17β-estradiol, as indicted by the values of the separation factor of isomers of 17β-estradiol/17α-estradiol (2.28) and the imprinted factor (3.01). Particle diameter of TFMAA-co-TRIM polymer was between 300 nm and 1.5μm, which suited well for solid phase sorbent throughout at low column pressure. The recognition of imprinting TFMAA-co-TRIM polymer for sterol molecules was driven by enthalpy eluted with acetronitile, and low temperature was in favor of the separation of sterol structure analogues on imprinting column. TFMAA-co-TRIM polymer possessed of determinate anti-heat stability, with melting point beginning at 255.84℃, Tp=257.40℃, control TFMAA-co-TRIM polymer and imprinting TFMAA-co-TRIM polymer were respectively decompounded at 267.79℃and 343.11℃, and solid micro-extraction noddle prepared by the special polymerization also showed definite recognition for 17β-estradiol by GC/MS detecting at 270℃. By selection of various washing and elution solvents, elution reagents of close polymerization system were of more advantage in template molecules retention and recognition on molecularly imprinted solid phase extraction column. At a certain extent, progesterone, 17α-estradiol, 17β-estradiol and 4-androstene-3, 17-dione could be intercepted on the MISPE column. Especially, MISPE had high selectivity for progesterone, and imprinting TFMAA-co-TRIM polymer could achieve adsorption balance within 50 min by absorption kinetics test for 17β-estradiol. However, MISPE column showed better selectivity and enrichment property for 17β-estradiol than C18 and CSPE columns according to the data from HPLC and GC/MS analyses. Recovery of 17β-estradiol on MISPE column was up to 85.5% while when prime extracting solution of milk powder was sampled, the recovery of CSPE and C18 columns were 43.7% and 30.7%, respectively.

通过研究阐明:紫外聚合产物TFMAA-co-TRIM中的νO-H振动吸收峰在聚合16h后红移,νC=O振动吸收峰在聚合24h后红移;TFMAA-co-TRIM对雌二醇异构体的印迹因子达到了3.01,α为2.28,优于其它功能单体参与得到的聚合物识别特性;TFMAA-co-TRIM聚合物粒径介于300 nm至1.5μm之间,作为色谱固定相具有良好的通量和低的柱压;在乙腈流动相中,TFMAA-co-TRIM分子印迹固定相的分离过程主要被焓驱动,低温有利于分子印迹固定相分离甾醇结构类似物;TFMAA-co-TRIM分子印迹聚合物在255.84℃时开始熔融,Tp=257.40℃,聚合物CP的降解温度在267.79℃,MIP降解温度在343.11℃,制备的固相微萃取头初步经GC/MS 270℃的耐热性测定;不同的洗提溶剂筛选证明了选择接近聚合溶剂的洗脱体系更有利于MISPE发挥识别效应;分子印迹固相萃取柱对孕酮、17α-雌二醇、17β-雌二醇和雄烯二酮都具有不同程度的保留特性,可作为此类化合物的吸附材料,特别对孕酮强保留的富集特性,可作为孕酮的选择性识别吸附剂,其分子印迹聚合物对17β-雌二醇的吸附动力学测定显示在50 min内基本达到吸附平衡,具有作为传感器核心敏感材料的潜力;对比萃取奶粉中17β-雌二醇性能,MISPE柱比非分子印迹固相萃取柱和C18柱具有更高的保留,回收率依次为85.5%,43.7%和30.7%。

On the basis of sea resources analysis, some argues of important scientific theory and technological assembly in the exploitation of oceanic resources are discussed in the paper, such as the technological assembly of deepsea creature resources commercial applications, the study on the hydatogenesis of sea bottom, the study on the reaction between water and rocks, the discriminating and exploitation technology of natural hydrate and solid metallic deposits.

在各种海洋资源可利用性分析的基础上,针对应用开发,提出的海洋资源开发中的重大科学理论与技术集成应引起科学界与管理部门的充分关注,包括深海生物资源商业应用开发的技术集成、海底热液成矿作用理论研究、水—岩反应研究、天然气水合物与固体金属矿产的识别与开采技术集成。

The separative criterion is important to the feature extraction and clustering analysis in statistical pattern recognition.

1引言可分离性判据在统计模式识别的特征提取和聚类分析中有重要应用犤1,2,3犦。

Hydraulic fracturing breccia can be joggled joint and the veins have clear fluid evidence. It is found that the fractures were developed in the crust around the uncompacted belt. Hydraulic fracturing experiments were conducted by using composite samples consisting of micrite and sandstone.

通过对大港滩海区沙一段下部岩心观察和薄片分析,识别出水力破裂角砾、砂岩脉、水力破裂裂缝及活化同沉积裂缝4种水力破裂作用产物,水力破裂角砾之间具有可拼合性,脉体具有明显流体作用痕迹;本区水力破裂发生于欠压实带周围的&壳&中。

The Bi-static Forward Scattering airier Radar can be used to detect, recognize and track the target with small detection probability in the low altitude sky, on the land or on the sea.

双基地前向散射栅栏雷达可以对低空、陆地或海面的低可探测性目标进行检测,识别和跟踪。

It was found out that the automation level of the drill and driver's operation level are also key factors which affect the drilling process.

本文通过对岩石的可钻性和钻进参数进行的分析研究,用已知岩性与未知岩性进行比较,能够得到相对的岩石特性,从而达到识别岩石性质的目的。

However, RFID stands for Radio Frequency Identification, and is also called as Electronic Label. It goes through radio signals to identify specific targets, and reading and writing information, as well as don't need identity system and specific targets to create such as electronic, optics or machinery. Properties of wireless communication, space-penetrating, information tracking and real-time location system/ RTLS will apply for increasing the system capability of safety and protection to Robot.

而「RFID, Radio Frequency Identification 」称之为「无线射频识别」,又称「电子标签」,是一种通信技术,可透过无线电讯号识别特定目标并读写相关资料,而无需识别系统与特定目标即可建立含电子、光学或机械接触,其特性:无线通讯、空间穿透性、资讯历程可追踪与「即时定位系统 RTLS, Real Time Location System」等,必然将成为机器人、机械手臂安全设计与防护的新应用。

This method,on one hand extractes the most efficient low features for lipreading,on the other hand,selectes features reasonably to improve their distinguishability.With 42-dimensional two-level visual features can get 77.8% rate of lip movement contents recognition for speaker-dependent cases.

此方法一方面提取了唇读的最有效的低级语义特征,另一方面更加合理地选择利用了特征的有效可区分性,使得用42维二级视觉特征,对特定人的唇动内容识别正确率达到77.8%。

Friend or foe digital transponders replace outdated technology with digital systems that improve reliability and maintainability.

敌我识别器将采用数字化系统取代原有的过时技术,提高了可靠性和可维护性。

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Since historical times,England ,where the early inhabitants were Celts, has been conquered three times .

从有历史以来,英国,在此地早期居住的是凯尔特人,已经被征服了三次。

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