可微性

This method is simple, accurate, repeatable and high selective for the determination of the active amylopsin in multi-enzyme mi...

该法便捷，准确可靠，重现性好，可用于多酶微片胶囊中胰淀粉酶活力的测定。

The conclusions were as follows: theνO-H andνC=O IR absorption maxima shifted towards higher wave numbers after imprinting 17β-estradiol on TFMAA-co-TRIM copolymer with the red shift of theνO-H groups being apparent after precipitation polymerization for 16h while that forνC=O groups being observed after polymerization for 24h. A strong interaction between TFMAA and 17β-estradiol was confirmed by the high selectivity for 17β-estradiol, as indicted by the values of the separation factor of isomers of 17β-estradiol/17α-estradiol (2.28) and the imprinted factor (3.01). Particle diameter of TFMAA-co-TRIM polymer was between 300 nm and 1.5μm, which suited well for solid phase sorbent throughout at low column pressure. The recognition of imprinting TFMAA-co-TRIM polymer for sterol molecules was driven by enthalpy eluted with acetronitile, and low temperature was in favor of the separation of sterol structure analogues on imprinting column. TFMAA-co-TRIM polymer possessed of determinate anti-heat stability, with melting point beginning at 255.84℃, Tp=257.40℃, control TFMAA-co-TRIM polymer and imprinting TFMAA-co-TRIM polymer were respectively decompounded at 267.79℃and 343.11℃, and solid micro-extraction noddle prepared by the special polymerization also showed definite recognition for 17β-estradiol by GC/MS detecting at 270℃. By selection of various washing and elution solvents, elution reagents of close polymerization system were of more advantage in template molecules retention and recognition on molecularly imprinted solid phase extraction column. At a certain extent, progesterone, 17α-estradiol, 17β-estradiol and 4-androstene-3, 17-dione could be intercepted on the MISPE column. Especially, MISPE had high selectivity for progesterone, and imprinting TFMAA-co-TRIM polymer could achieve adsorption balance within 50 min by absorption kinetics test for 17β-estradiol. However, MISPE column showed better selectivity and enrichment property for 17β-estradiol than C18 and CSPE columns according to the data from HPLC and GC/MS analyses. Recovery of 17β-estradiol on MISPE column was up to 85.5% while when prime extracting solution of milk powder was sampled, the recovery of CSPE and C18 columns were 43.7% and 30.7%, respectively.

通过研究阐明：紫外聚合产物TFMAA-co-TRIM中的νO-H振动吸收峰在聚合16h后红移，νC=O振动吸收峰在聚合24h后红移；TFMAA-co-TRIM对雌二醇异构体的印迹因子达到了3.01，α为2.28，优于其它功能单体参与得到的聚合物识别特性；TFMAA-co-TRIM聚合物粒径介于300 nm至1.5μm之间，作为色谱固定相具有良好的通量和低的柱压；在乙腈流动相中，TFMAA-co-TRIM分子印迹固定相的分离过程主要被焓驱动，低温有利于分子印迹固定相分离甾醇结构类似物；TFMAA-co-TRIM分子印迹聚合物在255.84℃时开始熔融，Tp=257.40℃，聚合物CP的降解温度在267.79℃，MIP降解温度在343.11℃，制备的固相微萃取头初步经GC/MS 270℃的耐热性测定；不同的洗提溶剂筛选证明了选择接近聚合溶剂的洗脱体系更有利于MISPE发挥识别效应；分子印迹固相萃取柱对孕酮、17α-雌二醇、17β-雌二醇和雄烯二酮都具有不同程度的保留特性，可作为此类化合物的吸附材料，特别对孕酮强保留的富集特性，可作为孕酮的选择性识别吸附剂，其分子印迹聚合物对17β-雌二醇的吸附动力学测定显示在50 min内基本达到吸附平衡，具有作为传感器核心敏感材料的潜力；对比萃取奶粉中17β-雌二醇性能，MISPE柱比非分子印迹固相萃取柱和C18柱具有更高的保留，回收率依次为85.5%，43.7%和30.7%。

The toxicity of spent potliner is discussed in this paper according to the research on main constitutes,microstructure,tiny section components,ash content and hydrating reactivity of SPL. The result indicates that the toxicity of SPL mostly rests with its leaching toxicity,contaminates in SPL are mainly dissolving fluorides and cyanides,these contaminates mostly come from electrolyze which infiltrate into the potliner and cause erosion,contents of dissolving F— and CN— in SPL are far more than the permitted discharge in national standard. SPL, which seriously does harm to eco-environment is classified as hazardous solid waste and should be detoxified as soon as possible.

本文从废槽内衬的物相组成、微观形貌、微区成分、灰分、水化反应性五方面研究了废槽内衬的危害性，结果表明，废槽内衬的危害性主要在于其浸出毒性，毒害物质主要是可溶氟化物和氰化物，毒害物质主要来自于电解质对内衬的渗透、侵蚀，废槽内衬中可溶F—和CN—的浓度远远高于国家标准规定的允许排放量，因此废槽内衬被认定为固体危险废弃物，严重危害生态环境，必须进行无害化处理。

Influnce of neutrophils in-to the walls of large epicardial coronary arteries in responsable to ischemia-reperfusion ［J］.

这种聚集可导致白细胞性栓子的产生，并可致微栓塞的发生［14］。

CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度；CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段；微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率，同时具有一定的靶向性，但总的转染范围仍然有限；采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预，发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低，CaN AβmRNA水平虽有下降趋势，但无统计学差异，提示质粒的用量及超声导入的参数有待进一步研究使其优化。

Based on the hypothesis of Brands, this thesis presents two off-line electronic cash system applied to small payment: one is an off-line nondivisible electronic cash system with complete anonymity, and the other is an off-line nondivisible electronic cashsystem with repealed anonymity. The two systems can solve the bottleneck problem of bank server. Furthermore, based on the concept of virtual debit as well as the payment demand of pay-per-use electronic commerce and mobile commerce, this paper designs a micro-system NPAY, which can solve the problem of extra-consuming, greatly reduce the system communication overhead, and the consumer can anonymously consume.

基于Brands假设，设计了两个适用于小额支付的离线电子现金系统：一个是具有完全匿名性的离线不可分电子现金系统，另一个是可撤消匿名性的离线不可分电子现金系统，这两个系统都较好地解决了银行服务器的瓶颈问题；针对即付即用(pay-per-use)类型电子商务和移动商务的支付需求，基于&虚借记&的概念，设计了一个微支付系统NPAY，较好地解决了消费者超额消费问题，系统的通讯开销也大大减少，并且消费者可以匿名消费。

Based on the hypothesis of Brands, this thesis presents two off-line electronic cash system applied to small payment: one is an off-line nondivisible electronic cash system with complete anonymity, and the other is an off-line nondivisible electronic cash'system with repealed anonymity. The two systems can solve the bottleneck problem of bank server. Furthermore, based on the concept of virtual debit as well as the payment demand of pay-per-use electronic commerce and mobile co mmerce, this paper designs a micro-system NPAY, which can solve the problem of extra-consuming, greatly reduce the system communication overhead, and the consumer can anonymously consume.

基于Brands假设，设计了两个适用于小额支付的离线电子现金系统：一个是具有完全匿名性的离线不可分电子现金系统，另一个是可撤消匿名性的离线不可分电子现金系统，这两个系统都较好地解决了银行服务器的瓶颈问题；针对即付即用(pay-per-use)类型电子商务和移动商务的支付需求，基于&虚借记&的概念，设计了一个微支付系统NPAY，较好地解决了消费者超额消费问题，系统的通讯开销也大大减少，并且消费者可以匿名消费。

The results showed that 7-day-precultured embryogenic calli had the best responses for bombardment among 2-7 day' preculture duration. Enhanced transformation efficiency was found in the explants treated with high osmotic medium 4h prior to till 24 h after bombardment. Particles of gold and tungsten were both found suitable for rice transformation and there was no adverse effect when tungsten particles were reduced to 1/3 standard amount for DNA precipitation on transient GUS gene expression. However, it was found that gold particle had higher GUS expression at 120 h post-bombardment. A preliminary microprojectile transformation system with an average of 15-20 blue spots/explant GUS expression was routinely obtained for 'Tainung 67' by using the particle gun with 1,100 psi rupture disk, 6 or 9 cm microcarrier flight distance and 4 mm of macrocarrier flight distance setting.

初步结果显示，以预培养2~7日之胚性癒合组织逐日测试转殖率，以预培养7日之培植体表现最佳；培植体於转殖前4小时至转殖后24小时以高渗透压培养基处理，有助於转殖效率之提高；比较金、钨粒子对转殖率在转殖后48h並无不同，但转殖后120 h取样，则金粒子组有较高之蓝点数；钨粒子用量可减少至1/3標准用量对转殖率並无影响；利用基因鎗在使用1， 100 psi裂碟片、大载片至挡片之距离为4 mm、微载髁至射击目標之飞行距离6或9 cm之设定，GUS基因表现之平均蓝点数均可达15~20个／培植体。

When the undrawn films were annealed atthose temperatures, the morphologies in the lamellar crystals had change. WAXDand iR studies shown that the crystallinities incrcand, dimensions in (002) and(020) normals increased while that in (200) fold had slight decreases. The rela-tionship between the orientation of the drawn UHMWPE gel-films and the struc-ture changes and the optimum drawing temperature had been disscussed.

通过IR及X－ray衍射等方法研究发现，拉伸前用不同温度退火处理后，凝胶膜的片晶微结构产生明显变化，在对应于超高拉伸温度范围内，其结晶度增加，微晶的c和b轴方向尺寸变大，而a轴方向的尺寸减小，从理论上对这一变化过程作了考察，并讨论了超高拉伸取向性与这些结构变化的关系以及可超拉伸取向性的最佳温度范围。

It is concluded that the positive rate of platelet antibody screening is very high in patients with hematologic malignancies, the coincidence rate of platelet antibody crossmatch in 249 blood samples is between 40% and 48%, and the effeciency of using crossmatched platelets in clinic is enhanced significantly.

微柱凝胶免疫分析技术在血小板抗体筛选中的应用大部分免疫性血小板减少性紫癜患者血小板膜上可测出血小板相关抗体，包括IgG、PA IgM、PA IgA，其中一项出现阳性，即为血小板抗体检测阳性，而且其量往往与外周血的血小板数量呈负相关，因此检测这种血小板相关抗体有助于免疫性血小板减少性紫癜的诊断［1］。

If you are unfortunate enough to the lovelorn, please tell me, I will help you out, really, please contact me!

如果你不幸失恋了，请告诉我，我会帮助你摆脱困境，真的，请联系我啦！

China's plan to cut energy intensity by 20 percent and pollutant discharges by 10 percent between 2006 and 2010 is a case in point.

中国计划在2006年到2010间降低20%的能源强度和减少10%的主要污染物排放，就是一个这样的例子。