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Mutation of the gARID1 binding site in the cwp1 promoter resulted in a decrease of promoter activity and mRNA levels during vegetative growth and encystation, suggesting that the gARID1 binding sites are positive cis-acting elements of the cwp1 gene promoters in both trophozoites and encysting cells. Using cotransfection assay, we also found overexpession of gARID1 can increase luciferase activity and mRNA levels, while mutation of the gARID1 binding sites of the cwp1 promoter resulted loss of this transactivation.

我们也用萤光酵素基因接上cwp1基因启动子,如果将其cwp1启动子上的gARID1结合位置突变,也可以发现萤光酵素和讯息RNA的表现有明显的下;再用cotransfection assay,可发现gARID1会使萤光酵素和讯息RNA表现明显上升,然而如果将其cwp1启动子上的gARID1结合位置突变,这种转活化则消失。

For constructing strong combined promoter to enhance the foreign gene expression in Fowlpox virus vector, four oligonucleotides were synthesized according to the essential structure of the vaccinia virus nature promoter P7.5 and were used for generating early and late promoters.

要提高外源基因在鸡痘病毒载体的表达量,必须使用强启动子来控制表达。为构建较强的启动子组合,根据痘苗病毒天然启动子P7.5的结构,人工合成4条寡核苷酸,形成一个早期启动子和一个晚期启动子,其首尾可相互连接。

The results showed that human transferrin could be expressed in these cell lines and mouse or goat mammary gland following transfection, and its expression level could be improved dramatically when it was driven by rabbit enhancer and promotor as compared with goat beta lactoglobulin promotor. Transgenic mice were generated by either microinjection or lentivirus infection.

结果显示所有表达载体均可指导人转铁蛋白在细胞以及小鼠和山羊乳腺中表达,兔转铁蛋白增强子与启动子组合可显著性增加人转铁蛋白的表达水平,且其指导表达的效率明显高于山羊β乳球蛋白启动子。

Here, we inserted the RRD3 fragment into the plant promoter-capture vector, pCAMBIA1391Z, and examined whether the RRD3 fragment has promoter activity in plants. Transgenic tobacco and rice calli both showed β-glucuronidase activity, indicating that RRD3 can act as a promoter in both monocot and dicot plants.

我们将RRD3片段插入到植物启动子捕获载体pCAMBIA1391Z中,检测RRD3片段是否在植物中也有启动子活性,转基因烟草再生植株和水稻愈伤组织均显示了gusA基因的表达,表明其在单子叶和双子叶植物中均可行使启动子功能。

5 Using" Y—Δ"for motor enablement avoids the accident, which could be chose according requirements of the users.

电机启动可采用&Y—Δ&转换方式,从而避免直接启动导致的不良后果。软启动装置可根据用户需要选配。

PPARα ligands suppressed OPN promoter actiity, and an actiator protein-1 consensus site conferred this repression. Oerexpression of c-Fos and c-Jun reersed the inhibitory effect of PPARα ligands on OPN transcription, and, in chromatin immunoprecipitation assays, PPARα ligands inhibited c-Fos and phospho–c-Jun binding to the OPN promoter.

PPARα配体可抑制OPN启动子活性,一个激活蛋白-1的一致序列位点与这种抑制有关。c-Fos和c-Jun的过表达可逆转 PPARα配体对OPN转录的抑制作用,并且在免疫沉淀实验中,PPARα配体可抑制c-Fos及磷酸化c-Jun与OPN启动子的结合。

It s tersion is greater in starting state ,and smaller in normal state moving.

此装置在皮带机启动阶段,能提供足够大的启动张力;启动完毕后,又可使皮带的张力恢复到额定值以维持皮带机的正常运行。

Working in this way, the problem to preheat the filament before starting the lamp would be solved, yet the power dissipation of starting circuit during the entire period of lamp lighted would dissipate no more.

智能预热启动是这样的:通电后,先对电极灯丝进行几秒时间的预热,然后将灯启动点亮,灯亮后启动电路自动关闭,这样既解决了可将灯丝先预热后才启动点亮灯的问题,又解决了灯在燃点过程中,启动电路仍要消耗功率的问题。

The PTZ plasmid vector was changed to plasmid vector pSP72, which contains both T7 and Sp6 promotors for transcription of antisense (T7) and sense (Sp6) RNA probe in vitro. The results revealed the label rate of cRNA probe is high, the hybrids between RNA-RNA are stable, so the background of hybridization is satisfactory.

本实验将质粒载体PTZ转换为带有T7和Sp6两个启动基因的pSP72载体,故可在体外转录cRNA时同时得到意义(Sp6为启动基因)和反意义(T7为启动基因)探针,实验结果表明,cRNA探针标记率高,杂合物稳定,故所获杂交反应背景较为满意。

These include giving the Fed expanded powers to regulate market stability, combining the Office of Thrift Supervision and the Office of the Comptroller of the Currency to strengthen regulation by reducing duplication, centralising the scrutiny of mortgage origination, creating an optional federal insurance charter, beginning the process of integrating the Securities and Exchange Commission and Commodity Futures Trading Commission and continuing to improve arrangements for clearing and settling over-the-counter derivatives, including development of well regulated and prudently managed central clearing counterparties for OTC trades.

这些改革举措包括:扩大美联储的权力以监管市场稳定;合并美国储蓄管理局和美国货币监理署(Office of the Comptroller of the Currency),通过减少重合来强化监管;集中对抵押贷款来源的审查;创建一个可选择的联邦保险宪章;启动整合证券交易委员会(Securities and Exchange Commission)和商品期货交易委员会(Commodity Futures Trading Commission)的程序;继续改进清算、结算场外衍生产品的安排,包括针对场外交易,发展得到充分监管和审慎管理的中央清算对手机构。

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