可以

In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

We may showcase our charm during school activities or interpersonal relationships; we may even contribute ourselves without any fanfare, possibly known only to yourself, yet you are excited privately for quite some time.

我们可以在课业、学习上做得不同，当然不是那种不同寻常的糟糕，可以是不同寻常的新方法，可以是不同寻常的新观点；我们可以在校区活动、人际交往中展现自己的风采：甚至，我们可以默默地发挥自己的光热，可能就你自己清楚，但是心里可以暗自兴奋好久。

We may perform differently in our lessons and studies, obviously not the unusually terrible sorts, I am referring to the extraordinary new method or new concept. We may showcase our charm during school activities or interpersonal relationships; we may even contribute ourselves without any fanfare, possibly known only to yourself, yet you are excited privately for quite some time.

我们可以在课业、学习上做得不同，当然不是那种不同寻常的糟糕，可以是不同寻常的新方法，可以是不同寻常的新观点；我们可以在校区活动、人际交往中展现自己的风采：甚至，我们可以默默地发挥自己的光热，可能就你自己清楚，但是心里可以暗自兴奋好久。

For example, pass Internet, we give an afar of the friend perhaps relatives send letter an interest, several seconds inside can arrive;We can mutually confabulation, even can meet each other;We need not egress can with shopping;Our canning be foolish can do business with foreign company at home.

例如，通过因特网，我们给一个在远方的朋友或者亲戚发信息，几秒钟之内就可以到达；我们可以相互交谈，甚至可以互相见面；我们不用外出就可以购物；我们可以呆在家就可以跟外国公司做生意。

Disneyland is an amusement park,but we can also call it a theme park.it has all the normal attractions that you can find at an amusement park,but it aleo has a theme.the theme,of course,is Disney movies and Disney characters.for example,you can find a roller coster in most amusement parks,but in Disneyland,the theme of the roller coaster is Disney characters.this means that you can find Disney characters all over the roller coaster.you can also watch Disney movies,eat in Disney restaurants,and buy Disney gifts.and you can see Disney characters walking around Disneyland all the time

迪士尼乐园是一个娱乐公园，但我们也可以把它称为主题园林有正常的景点，你可以找到一个游乐园，但它aleo有theme.the主题，当然是迪斯尼电影和迪士尼characters.for。例如，你可以找到一个辊coster大多数游乐场，但在迪斯尼乐园，主题的过山车迪斯尼characters.this意味着你可以看到迪斯尼人物在滚筒coaster.you也可以看迪斯尼电影，吃在迪斯尼的餐厅，买迪斯尼gifts.and你可以看到迪斯尼人物的迪士尼乐园

In a remote mountains, have the hunter with a first-rate marksmanship, one day, he hunts into hill, going, encounter a batch of wolves suddenly, the hunter lifts a gun to want to hit, be in this is wolf mouth conversation, it is OK that you hit me, but I have a condition, the hunter says then you say, the wolf says you can hit me 3 guns, if you are hit,do not die me, do you make I ate you OK, it is OK that the hunter listens to have very much confidence ground says, the hunter opens two guns repeatedly, see a wolf still stand over, the hunter is very amazed, opened the 3rd gun again then, wolf or station are over there, the hunter sees him be defeated pull, say to the wolf you can eat me to pull, the wolf looks say you should take off the dress pull me eating you, the hunter agrees to pull, take off the dress then pull, when taking off last briefs, the wolf sees pull make off at once, running, the wolf encounters one countrywoman, countrywoman asks a wolf, you run what, the wolf says the thing that bets on hill to be pulled, did not think of to there still is a gun below him, countrywoman listens, the 2nd gun that tells a wolf him but very fierce, 10 years ago, I was hit by him one gun, cut still does not have heal now.

导读：在一座深山里，有一个枪法非常好的猎人，有一天，他进山打猎，走着走着，突然遇到一批狼，猎人举枪便要打，就在这是狼开口说话，你打我可以，但我有一个条件，猎在一座深山里，有一个枪法非常好的猎人，有一天，他进山打猎，走着走着，突然遇到一批狼，猎人举枪便要打，就在这是狼开口说话，你打我可以，但我有一个条件，猎人说那你说吧，狼说你可以打我三枪，如果你打不死我，你就让我吃了你可以吗，猎人一听很有信心地说可以，猎人连开两枪，一看狼还站在那里，猎人很吃惊，于是又开了第三枪，狼还是站在那里，猎人一看自己输拉，就对狼说你可以吃我拉，狼一看说你应该把衣服脱拉我在吃你，猎人同意拉，于是把衣服都脱拉，脱到最后一件内裤时，狼看到拉撒腿就跑，跑着跑着，狼遇到一村妇，村妇问狼，你跑什么，狼就把山上打赌的事说拉一遍，没想到他下面还有一棵枪，村妇一听，告诉狼他的第二棵枪可很厉害呀，十年前，我被他打了一枪，伤口现在还没愈合呢。

First, to objects, father can sue mother, son can sue father, grandfather can sue grandson, fancy woman can fancy man, beggar can sue passer …… Anyway, you can sue anyone or any organization in court.

说起来真是搞笑，因为，首先从对象来说，在美国，爸爸可以告妈妈，儿子可以告老爸，爷爷可以告孙子，情妇可以告情夫，乞丐可以告路人……总之，你可以告任何你想告的人和机构。

I'm the Fireman Fire, Fa, Fireman I got that fire I'm hollering I got that fire come and try me and You can spark it up and I'ma put you out You can spark it up and I'ma put you out [Verse 1] Ain't nobody fuckin with me man, Heatman Ski Mask spending next weeks cash, he fast And I don't even need a G pass I'm pass that I'm passing em out now and you can't have that And my chain Toucan Sam That tropical colors you can't match that Gotta be abstract You catch my gal legs open betta smash that Don't be surprise if she ask where the cash at I see she wearing them jeans that show her butt crack My girls can't wear that why, that's where my stash at I put my mack down that's where you lack at She need her candlelit and I'ma wax that I rekindle the flame She remember the name It's Weezy Baby January December the same Mama gimme that brain Mama gimme that good Cause I'm the fireman You hear the firetruck I'm the Fireman Fire, Fa, Fireman I got that fire I'm hollering I got that fire come and try me and You can spark it up and I'ma put you out You can spark it up and I'ma put you out Fresh on campus it's the Birdman Jr Money too long teachers put away ya rulers Raw tune not a cartoon No shirt, tattoos, and some war wounds I'm hot but the car cool She wet that's a carpool Been in that water since a youngin you just shark food Quick Draw McGraw I went to art school Yeah the lights is bright but I got a short fuse Don't snooze Been handling the game so long my thumb bruise Ya new girlfriend is old news Yeen got enough green and she so blue yeah Cash Money Records where dreams come true Everything is easy baby leave it up to Weezy Baby Put it in the pot let it steam let it brew Now watch it melt don't burn ya self I'm the Fireman Fire, Fa, Fireman I got that fire I'm hollering I got that fire come and try me and You can spark it up and I'ma put you out You can spark it up and I'ma put you out Ridin' by myself well really not really So heavy in the trunk make the car pop-a-wheelie Who?

我是消防员消防，花园，消防员我接到火警我喊叫我接到火警来试试我，它可以激发起来，我是把你出去它可以激发起来，我是把你出去 [诗歌1] 不与我没有fuckin人，Heatman 滑雪面具的现金支出今后几周，他快我甚至不需要一个G通过我传递我现在传递时间内，你不能有链巨嘴鸟和我的山姆这种热带色彩不能匹配肯定在抽象你明白我的加仑双腿粉碎的开放斗鱼不要惊讶，如果她问其中现金我看到他们，她穿着牛仔裤，显示她的屁股裂缝我的女孩不能穿这个原因，这就是我的藏匿在我把我的下这就是你缺乏麦克她需要她的烛光和我是一个蜡余点燃圣火她记得这个名字这是12月1月Weezy婴儿一样妈妈gimme，大脑妈妈gimme，良好原因我是消防员你听到救火车我是消防员消防，花园，消防员我接到火警我喊叫我接到火警来试试我，它可以激发起来，我是把你出去它可以激发起来，我是把你出去新鲜校园它是鸟人Jr 钱太长教师收起亚统治者原料调整不是一个卡通没有衬衫，纹身，和一些战争创伤我觉得又热，但赛车冷却她湿这是一个用车在这水自1 youngin你只鲨鱼的食物快速绘制麦格劳我去艺术学校是的，灯光明亮而我得到脾气暴躁不要打盹被处理的游戏，只要我的拇指挫伤亚新女友是旧闻陈吕令意有足够的绿色和蓝色是啊，她这样现金货币记录下梦想成真一切都留给孩子容易到Weezy婴儿放入锅蒸让它让它酿造现在看它融化不烧亚自我我是消防员消防，花园，消防员我接到火警我喊叫我接到火警来试试我，它可以激发起来，我是把你出去它可以激发起来，我是把你出去我自己也实在没有真正因此，在主干沉重让赛车弹出一，离地平衡特技谁？

We meet in this game This is still infatuated with a network Let me be your friends one by one to leave the game To the pursuit of real-life time Then there is a fishes persist in the conviction Let me come to the so-called final Finally, we have a full class to We also become a game of a husband and wife Together to chat together to fight together to see the scenery Appear together in front of friends I have told you several times whether I would like to leave the game But do not know what I come back time and again the Now, I once again decided to leave the game I do not know how long this can leave I do not dare In fact, said to leave the game say that the discussion was not as good as you After the full level because I slowly began to weary of the game Do you have is a day I insist on the belief line Recently, I go to bed every night before you would like to But not like before, like the game's equipment But in reality you imagine how a person What kind of character, and so there We will occasionally illusion of reality can come together The game took a quarter of my college life time Reform is the time to leave As in real life too cruel I felt pressure brought about by the reality Whether in life or learning I really like this now become a regular university, said the University graduate unemployment is equivalent to That is not what I want I am not worried that I can not find a job after graduation Not because of the need to Go on like this I do not even know that after I graduated I can do what I do Looking for a job on it is an impossible dream of Do you still remember I remember a long time ago I asked you I ask you to do what - the sun - the stars - the moon I have become a game where you have the sun I hope I can become your reality stars I would also like to leave after you I hope you will be able to chat with me in the past I hope we can do Haojuhaosan The way the election is himself Life must continue

我们在这场比赛中相遇这是与网路仍痴心让我成为你的朋友们一个接一个，去游戏现实生活时间的追求然后有一个鱼类坚持信念让我谈谈所谓的决赛。最后，我们必须以一个完整的类我们也变成了一个游戏的丈夫与妻子一起，一起聊天，争取一起看风景前面的朋友在一起显示我已经告诉你几次是否我想离开游戏但不知道什麼我回来一次又一次，现在，我又一次决定离开游戏我也不知道多久这可以离开我不敢其实，说要离开讨论不是与你的一样好的游戏说后全部级别因为我慢慢开始厌倦游戏你是我坚持的信念行的一天最近，我去睡觉每天晚上之前您想要但不是象以前，像游戏装置但在现实中你就可以想像如何一人什麼样的字元，并因此有我们偶尔会现实的幻想可以走在一起这场比赛拿了我的大学生活时间的四分之一改革是离开的时候在太残酷的现实生活中的作为我觉得压力带来的现实无论是在生活或学习我真的这样现在成为一个说的正规大学，大学毕业生失业等於这是不是我想要的我不忧虑我不能毕业后找到一份工作不因为的需要这样下去我甚至不知道，我毕业后我可以做我做些什麼找一份工作就是一个不可能的梦想的你还记得吗我记得很久以前我就问你我问你做了什麼--星星-太阳月亮我已经变成一场游戏，在有阳光的地方我希望我可以成为你的现实星星我亦想在此之后你离开我希望您将能够在过去与我聊天我希望我们可以做 Haojuhaosan 选举方式是自己必须继续生活

In this paper, firstly, not only the incidence matrix ,adjacent matrix, cycle matrix, cut-set matrix of an undirected graph are summarized, but also the close contact between a graph and its corresponding matrix are discussed ; secondly, many problems of a graph which are solved by analysing its matrix are listed as follows:1、The co-tree set of a graph is obtained by using its cycle-matrix ; 2、The branches of its spanning tree are given by using its cut-set matrix ; 3、By making use of the incidence matrix of a graph ,not only its vertex cut 、cut vertex 、isolated point and spanning tree can be obtained ,but also the two sides which are whether parallel or not can be judged ;4、By using their adjacent matrix ,the two graphes which are whether isomorphous or not can be judged; once more, there is a detailed introduction in view of special graph (for example: bigaritite graph ,regular graph and so on);last but not least, a graph method of calculating the N power of a matrix is given and the practical applications of the theorem for degree is indicated.

本文首先综述了无向图的关联矩阵，邻接矩阵，圈矩阵，割集矩阵以及图和它对应矩阵之间的关系；其次总结出了利用上述各类矩阵可以解决的图的若干问题：1、利用图的圈矩阵可以求其连枝集；2、利用图的割集矩阵可以求其生成树的树枝；3、利用图的关联矩阵不仅可以求其割点、点割集、连通度、孤立点和生成树，而且可以判断两条边是否平行；4、利用图的邻接矩阵可以判断两个图是否同构；再次，针对特殊图（例如：二分图、正则图等等）的邻接矩阵作了详细介绍；最后，得到了利用图计算矩阵的N次幂的方法，指出度数定理的实际应用。

On closer examination, though, this is not a vote for multilateralism but just the opposite.

仔细审视后我们发现，这并非是对多边主义投出的赞成票，而是恰好相反。

Uncovering their weak spots, so I can defeat them.

揭露出他们的弱点，这样我就可以打败他们了。