受精卵

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

One of two sperm get into an archegonium and fused with egg. The nucleus of fertilized egg began to divide, and the walls were formed after the third division. The eggs without fertilization divided two times and then disappeared.

雄配子体产生的两个精子中的一个进入颈卵器与卵细胞结合形成受精卵，受精卵核到第3次分裂形成细胞壁，没有受精的卵细胞还要进行两次有丝分裂后才消失。

Ultraviolet rays have an effects on the development of seminal eggs of Ascaris lumbricoides.

为了解紫外线对蛔虫受精卵发育的影响及能否杀灭蛔虫受精卵，我们进行了本研究。

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

Cross of the two species showed hybrid weakness in both fertilization and growth, while zygotes'hatching success was similar with parental combinations, as well as the larvae survival which exist no significant with Kumamoto oyster larvae. Hybrids died numerously when metamorphosing and setting on the cultch. The survival hybrid spats grow poorly with high mortality.

杂交幼虫在受精率上存在显著劣势；但是受精卵的孵化率与纯种受精卵无显著差异；杂交幼虫在生长上也存在显著劣势；而存活率与熊本牡蛎无显著差异；杂交幼虫附着变态期间大量死亡，存活下来的稚贝生长缓慢，死亡率高。

Will not be fertilized, and no implantation of the fertilized eggs of the naked eye can not see.

不会是受精卵的，没着床的受精卵肉眼是看不到的。

AKT1 is the necessary for the development of mouse fertilized eggs.

1、在小鼠受精卵的中，AKT1与AKT2 mRNA的表达不同，AKT1在受精卵的发育过程中是必需的。

Crayfish Procambarus clarkii zygote belongs to mesolecithal eggs, and cytoplasm were pushed aside lied in the superficial part of the egg.

结果表明，克氏原螯虾受精卵属于中黄卵，细胞质被排挤到受精卵一侧，位于受精卵表面。

The healthy survivals were also transferred into the oviducts of pseudopregnant female mice. PCR was used to analyze the integration of the transgene in the genomes of mice.

选用内交系C57BL／6J种小鼠作为受精卵的供体鼠，昆明种小鼠作为假孕母鼠，将长2583bp、携带有CMV启动子和人CKLF-1基因的片段显微注射到受精卵的原核内，然后将注射后成活且健康的受精卵移植到假孕母鼠的输卵管内使其发育。

Six hundred ova were transfered into twenty-nine pseudopregnant recipients. Fifty-five offspring were born from the ova mircroinjected with the Bcl-2 fragment expression cassette.Twelve of them were proved to be gene integration positive by PCR .Four founder mice,two male and two female ,were further confirmed to be transgenic by Southern hybridization.

用BglII+SalI+PvuI三酶切pWBS和用BglII+BamHI双酶切pWCS，分别回收并纯化3.0kb的基因片段WAP-Bcl-2-SV40polyA和3.5kb的基因片段WAP-c-myc-SV40poly，作为目的基因，通过受精卵显微注射技术，分别导入C57BL／6J雌鼠与DBA／2J雄鼠交配的F1代小鼠受精卵的雄原核中，各注射约1000枚，将存活的受精卵各600枚分别移植至29只假孕母鼠输卵管壶腹部内。

It goes back to what I told you...

现在回到我告诉过你的。。。

With a supporter in Mr Charest and an admirer in Mr Dumont, Mr Harper may be encouraged to call an election himself.

由于在沙雷那边有个支持者，杜蒙那边有个崇拜者，鼓励哈珀为自己举行一次选举。