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厚的切片

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The result show in transplanted vessel EF-1 and PCNA are mainly expressed in smooth muscle cells and Clemons rate proliferation of SMC is the core of endothelium proliferation.In the third part of our experiment we use fluid nitrogen to fire transplanted rein, crofter tissue homogeneous to test the expression of CE and EF-1 gene we use RT-Craned immunohistologiccal method to test mKNA and protein level respectively and analyze the expression of these tm genes in transplanted vein.

本实验的第二部分,我们将术后大鼠分为10组,每组8只,分别于术后6hours、24hours、72hours、1W、10days、2W、3W、4W、6W、8W切取移植静脉,将切取的不同时期的移植静脉分别置于10%中性福尔马林溶液中固定和液氮冷冻固定,将福尔马林溶液固定的标本制成4μm厚石腊切片,,常规进行病理观察大体改变,静脉标本HE染色后于光镜下观内膜增殖情况,并应用PCNA单克隆抗体,观察SMC增殖情况。

In addition,the edge were easily teared radially kuring emulsification and implantation following diathermy capsulotomy.

结果 病理切片显示:电子环形撕囊的前囊膜反卷、增厚重叠在一起,晶状体上皮细胞排列失去规则。

Aortic perfusion of a fixative solution. Coronal sections were cut at 25 μm thickness on a freezing microtome and up to five series of adjacent sections were collected for nNOS immunohistochemical processing using streptavidin????

应用Morris水迷宫测试系统检测大鼠空间参考记忆获得及维持的变化;②海马的nNOS免疫组化研究:PSD 24,48和72 h后,立即取脑、固定、行冰冻切片(片厚25 μm)、SABC法免疫组化染色、光镜下进行观察分析、摄像。

Coronal sections were cut at 25 μm thickness on a freezing microtome and up to five series of adjacent sections were collected for nNOS immunohistochemical processing using streptavidinbiotinperoxidase complex technique.

应用Morris水迷宫测试系统检测大鼠空间参考记忆获得及维持的变化;②海马的nNOS免疫组化探究摘要:PSD 24,48和72 h后,立即取脑、固定、行冰冻切片(片厚25 μm)、SABC法免疫组化染色、光镜下进行观察分析、摄像。

This displayed the serial transversal, coronary and sagittal sectional anatomy of the oculomotor nerve, trochlear nerve, trigeminal nerve, abducent nerve, facial nerve, vestibulocochlear nerve and lower cranial nerves and their related structures, which provides basis of sectional anatomy for imaging study of the cranial nerves.

采用生物塑化技术,制作了层厚为1.0mm的薄层断面切片,系列地显示了动眼神经、滑车神经、三叉神经、外展神经、面神经-前庭蜗神经、低组脑神经及其相关结构的横断、冠状和矢状位薄层断面形态,为脑神经的影像学研究提供了对应的薄层断面解剖学基础。

Results: The atherosclerotic model was successfully established in rabbits. T1 WI, T2 WI and PDWI sequences scanning showed thickening of aortal wall and the plaque showed equal, high or low signal intensities, which was in good accordance with the pathological examination results. The atherosclerotic plaques were successfully detected and analyzed by MR and MR had higher accuracy than ultrasound examination.

结果:成功建立兔动脉粥样硬化模型;MR图像T1、WI、T2、WI、PDWI表现为兔动脉管壁增厚,斑块呈等信号、高信号或低信号,与病理切片检测结果符合率较高;MR成功检测斑块并分析其成分;与超声比较,MR具有较高的准确性。

Gloeosporioides. The soluble protein of Stylosanthes accessions resistant and susceptible anthracnose was no function to C. gloeosporioides. Leaves structure of two accessions was observed with method of paraffin section.

利用石蜡切片观察,对叶片结构进行分析表明,Mineirao柱花草与CIAT2312柱花草相比,Mineirao柱花草的上表皮细胞较厚于CIAT2312柱花草。

The lung tissue was cut to 4μm sections, deparaffinized and rehydrated, which were subjected to immunostaining using avidin -biotin -complexed method according to standard procedures.

取厚4μm的肺组织切片经过脱蜡、水化,严格按照免疫组织化学ABC试剂盒说明书操作。

Mathematical model of hydrodynamic action in the free abrasive wire saw slicing process was established.

建立了游离磨料线锯切片过程中流体动压效应的数学模型,并采用有限差分法进行了数值分析,得到了游离磨料线锯切片的流体动压力分布和膜厚。

Methods: Paraffin (7 μm in thickness) and methacrylate resin (25 μm) embedded renal sections were observed using 40× objective lens and 100× oil lens, respectively, and the volume fractions and diameters of the renal tubules and the tubule nuclei were estimated with stereological methods.

分别用高倍镜(40×物镜)和油镜(10 0×)观察石蜡(7μm厚)和甲基丙烯酸树脂(2 5 μm厚)包埋的肾脏切片,利用体视学方法测量肾小管的体积分数和直径以及肾小管细胞核的体积分数和直径。

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