卵黄膜

Oogonium develops into early oocyte in the ovary, and then the oocyte leaves the ovary for the coelomic fluid in the form of single cell or cell mass followed by the rapid separation of the group of oocytes into individual ones. Oocyte enters into the nephridium after its maturation. The rupture of germinal vesicle marks the oocyte maturation. Oocyte in the coelom does not have follicle membrane and vitelline membrane is formed and developed by the oocyte itself. Smaller oocyte (0μm in diameter) is round, and larger ones (≥60μm in diameter) is ovate. The short and long diameters of a morphologically mature oocyte are about 115—120μm and 140—145μm respectively, and the vitelline membrane is 7—9μm thick.

卵原细胞在卵巢中发育至早期卵母细胞时期单个或成团脱离卵巢入体腔液中，卵母细胞团细胞很快分离为单个细胞；卵母细胞在体腔液中发育成熟后进入肾管；生发泡破裂是卵母细胞成熟的标志；体腔中卵母细胞无滤泡膜，卵黄膜的形成与发育靠卵母细胞本身；卵径小于60μm的卵母细胞呈圆形，卵径大于60μm 的卵母细胞为卵圆形，形态上成熟的卵母细胞短径约115—120μm、长径约140—145μm、卵黄膜厚7—9μm。

The cortical alveolar exudate is involved in the transformation of the VE into the FE during the process of fertilization.

观察到，太平洋鲱鱼卵黄膜由5层组成，皮层小泡参与了受精膜的形成，在卵黄膜转化为受精膜的过程中，5层都发生不同程度的形态和糖蛋白及PAS阳性物质的变化。

In ovary, PNA receptors appeared in the oocyte cytoplasm of second phases of oogenesis; the positive granules gradually increased from third to forth phases, and they exhibit a maximum expression before vitellogennic stage in cytoplasm of oocyte; from vitellogennic stage to chorionation stage, the positive granules gradually reduced. Binding sites on follicle cells were changed with their morphological variation in every stage of oogenesis.The staged and specific expression of oncogene c-kit, the tyrosine-kinase receptor, is closely related with gametogenesis.

2在中华蚱蜢卵子发生的早期，PNA受体是由卵母细胞自身合成；在卵黄发生前的准备时期，卵母细胞质中的PNA受体一部分来源于自身合成，另一部分则来源于滤泡细胞，而糖复合物的大量出现可能直接与卵黄发生有关；卵黄发生期PNA受体的减少可能是在卵黄物质形成中部分发生了修饰；卵黄和卵壳的相继形成过程中，阳性反应的出现说明PNA受体参与了卵黄膜及卵壳的形成，而这些受体物质是由滤泡细胞分泌的。

Under light microscope, the egg is an oblated and agglutinated demersal with a narrow perivitelline space; eggs ranged in diameter from 1.95 to 2.38 mm with a mean of 2.18 ± 0.03 mm; their membrane are smooth and they have 5 to 7 cannular keratose egg-filaments; the oil globules are multiple, ranged in diameter from 0.05 to 0.50 mm and in number from 7 to 145 with a mean of 28±2, the position of them migrate during embryonic development; the yolks are ivory-white with no segments, ranged in diameter from 1.00 to 2.30 mm with a mean of 1.90 ± 0.03 mm; the development phases of the embryo are variable. So they were identified as the eggs of one Beloniformes.

在光学显微镜下：卵呈微扁圆形，卵径1.95~2.38mm，粘着沉性；卵黄间隙较窄，卵黄囊呈乳白色，无龟裂；卵膜平滑，在其表面有5~7根细长角质管状卵膜丝；多油球数不定（4~145），油球径0.05~0.50mm，油球在卵黄囊中的位置随不同的发育时期而不断发生变化；胚体处在不同的发育阶段，其特征符合颌针鱼目鱼卵的特征。

According to the size and shape of oocyte, the morphology of nucleolus, the growth of yolk and the structure of follicle, oogenesis of H. d. supertexta can be divided into three stages as follows: oogonium, previtellogenic oocyte and vitellogenic oocyte. The ovary wall is composed of tunica adventitia and germinal epithelium which will produce oogonia and follicle cells. The follicle is the structure unit of ovary.

根据卵细胞的大小、形状，核仁的形态，卵黄颗粒的积累情况，滤泡的结构等，将九孔鲍卵子的发生分为卵原细胞、卵黄发生前的卵母细胞和卵黄发生期的卵母细胞3个时期；卵巢壁由外膜及内生殖上皮构成，生殖上皮分化产生卵原细胞和滤泡细胞；卵巢的结构单位是滤泡。

Yellow Sea ; Clupea pallasi ; vitelline envelope ; fertilization envelope ultrastructure ; glycoprotein ; PAS positive material ; dynamic change

黄海；太平洋鲱鱼；鲱鱼卵黄膜；受精膜亚微结构；糖蛋白； PAS阳性物质；动态变化

The hybrid between Brachymystax lenok and Hueho taimen were not a live in the past 2 years. However, the fertility and eyed rate of BH were significantly higher than that of HH and BB (P.05), while the hatching rate were significantly lower (P.05). The larvae died within 1 to 2 hours after hatching. It was observed that the tail of yolk-sac larve couldn't stretch around the yolk, and there were hyperemia in the middle and posterior segment of the tail. The reason why the hybrid between Brachymystax lenok and Hucho taimen couldn't snrvive might was the karyoplasms of the gametes from Brachymystax lerwk and Hucho taimen by distant hybridization were not compatible.

细鳞鲤×哲罗鱿的杂交经过2年多批次实验均未得到成活杂交子代，但在胚胎发育阶段BH的杂交受精率、发眼率均显著高于双亲自繁对照组(P.05)，但其孵化率显著低于双亲对照组(P.05)，BH杂交子代在破膜后1~2h内即死亡，刚破膜的仔鱼尾干中后段至尾鳍部分盘绕于卵黄囊表面不能伸展，且所有破膜仔鱼的尾干中后段均存在充血点，本研究认为，这种反交［细鳞鲑×哲罗鲑］子代不能成活的原因可能是由远缘杂交受精卵核质不相容所导致。

Suggestion: the coatings were an apparatus to control the infolding of the plasma membrane during vesicle formation.

这些小泡失去它们的刚毛状的外被，相互融合形成大的膜包被的卵黄体，这是成熟的卵黄细胞的特征。

METHODS: Constructing the CAM model by exposing CAM on the shell subpoint between 2 vitelline veins and generating an air chamber after 5 days of incubated with aseptic incubator at 37℃.

将鸡胚消毒后，置于37℃无菌恒温箱中孵育5 d，在距胚头1 cm两条卵黄静脉之间的卵壳投影部位磨切暴露尿囊膜，制成假气室，用无菌滤纸封闭窗口，制成鸡胚尿囊膜模型，备用。

There was no typical Golgi complex in oogonia, but there were some Golgi vesicles. At the early stage of vitellogenesis, Golgi complex was abundant whose saccules increased to more than ten layers. At the middle stage of vitellogenesis, Golgi complex grew more in numbers and got together, so its secreting quickened. Moreover, it formed a number of Golgi vesicles, which were filled with granule substances that changed into yolk granule finally.

卵原细胞内无典型的高尔基复合体，但具高尔基囊泡；卵黄发生早期，高尔基复合体发达，膜囊数达10多层；卵黄发生中期，高尔基复合体分泌活动加快，产生大量的大小囊泡，囊泡内充满颗粒状物质，最后演变成卵黄颗粒。

Means the parent directory, so this command means to execute "toolchain.sh," which is in the current directory.

代表父目录，所以这个命令就是执行当前目录下的"toolchain.sh"。

Yes,In fact,I'm on our city ream.

是的。事实上我是我们市队的。