卵裂球

The surface of the egg opposite to the animal pole .

与卵黄偏度大体相对应，植物极区的卵裂球大形的场合较多。

There were 8 embryos single blastomere amplified, and the amplification success rate was 72. 7%(8/11); 3 embryos' not amplified; 2 embryos'showed preferential amplification , and the PA rate was 25%(2/8); and the 7 tubes of blank with washing cell solution were not contaminated. We transferred 3 unaffected embryos to the uterus. But unfortunately, blood hCG was negative after 2 weeks of transferring indicating no pregnancy.

共8枚胚胎的单卵裂球得到扩增，扩增成功率为72.7%（8/11），3枚未得到扩增，2枚发生了偏性扩增(preferentialamplification，PA)，PA的发生率为25%（2/8），细胞洗脱液空白对照7管均无污染发生。3枚诊断为正常的胚胎被移植入宫内，不幸的是，移植后2周，血hCG阴性，证明未妊娠。

Single blastomere PCR is stable and reliable,and it can be used for preimplantation genetic diagnosis.

目的建立单卵裂球PCR技术，为开展单基因病的着床前遗传学诊断奠定基础。

FISH technique could be performed for the detection of HBV in human blastomere cells,and it has great significance to reduce vertical transmission of HBV in IVF-ET.

荧光原位杂交技术可用于卵裂球细胞HBV感染的检测，在体外受精-胚胎移植实践中对降低HBV垂直传播有重要意义。

The cells of blastomere randomly distributed either embryonic parts or extraembryonic parts of blastocyst.

2-细胞胚胎卵裂球随机分布在囊胚的胚胎部分和胚外部分。

We also confirmed in this paper that it is injected somatic cells but not broken cytoplasm formed multi blastomere through mitosis in the reconstructed embryos.

在本实验中还通过用DNA染料（Hoechst-33342）对核移植体进行染色的方法，对融合后成纤维细胞在核移植体内不同时间的运行及发育模式进行了观察，确认了是成纤维细胞经有丝分裂形成多个卵裂球而不是由于细胞质碎裂形成的两个或多个碎块。

The mitotic error rate in cleavage stage embryos was proven to be higher than the meiotic aneuploidy rate and as a consequence, the genome of a single blastomere is not representative for the genome of the other cells of the embryo.

在卵裂期胚胎这种有丝分裂的错误率被证明是高于减数分裂的非整倍体错误率，因此，单个卵裂球的基因不能代表其他胚胎细胞的基因组。

Ovine immatured oocytes, matured oocytes and embryos from 2-cell stage to blastocyst produced in vitro were recovered, and RT-PCR was used to semi-quantify mRNA expression. Proteins of Cx43 and Cx45 were detected by using a combination of immunocytochemistry and Laser Scanning Confocal Microscope.

收集绵羊未成熟和成熟卵母细胞以及体外受精早期胚胎各发育阶段的卵裂球细胞，通过RT-PCR半定量检测Cx43和Cx45基因的mRNA表达量；通过免疫组织化学方法结合激光扫描共聚焦显微镜检测Cx43和Cx45蛋白在绵羊体外受精的早期胚胎发育过程中的表达情况及表达区域。

Indirect immunofluorescence was used to observe the distribution of fibronectin in human oocyte and 2-cell to morulae embryos.

纤维粘连蛋白均匀分布在体外未受精人卵母细胞和受精后人胚胎早期发育的不同阶段的卵裂球表面，各时期的免疫荧光强度未见明显差别。透明带未见纤维粘连蛋白表达。

Sowe selected the vinblastine optimum parameters were 0.1μg/ml and 8 hourcultured duration.The fifth, there were significantly differences between 0.45% sodium chloride groups and other two groups (0.5% sodium citrate groups and 0.35%potassium chloride groups) on the metaphases (P＜0.05), in the experiment of theeffect on the mouse 4-and 8-cell embryo stage single blastomere of differenthypotonic fluids, by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠8-细胞期胚胎单卵裂球染色体标本的长春花碱的处理浓度和时间是0.1μg／ml和10小时。5、在确定不同种类低渗液处理对小鼠4、8-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，0.45％氯化钠低渗液组与其他两组(0.5％柠檬酸钠和0.35％氯化钾)差异显著(P＜0.05)。

Chrysanthemum of 10 thousand birthday is lax to edaphic requirement, with the arenaceous qualitative loam with fecund, good drainage had better.

万寿菊对土壤要求不严，以肥沃。排水良好的砂质壤土为好。

He unstepped the mast and furled the sail and tied it.

他拔下桅杆，把帆卷起，系住。