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Blastomere numbers and morphology scores were highest in group Ⅰ(P.05), but no significant differences existed between group Ⅱ and group Ⅲ.

组的胚胎卵裂球数目及胚胎形态评分均显著优于Ⅱ组及Ⅲ组(P.05);Ⅱ组与Ⅲ组相比差异无统计学意义。

Hence, potentially viable embryos will be discarded upon screening a single blastomere.

因此,有可能对单个卵裂球的基因筛选导致一个有活力的胚胎被丢弃。

The repeated detection of single blastomere of untransferred embryos verified the PGD results, and further indicated that our PGD technique of ACH was relatively stable and reliable.

通过对未移植胚胎的单卵裂球的重复检测,验证了PGD的结果,进一步说明了我们开发的ACH的PGD技术是较稳定、可靠的。

F508 mutation detection on the single blastomere was performed with nested polymerase chain reaction.

同时利用套式聚合酶链反应技术,对活检所得的单个卵裂球分别进行ΔF508 突变基因检测。

BACKGROUND: Presently, the method of establishing human embryonic stem cells mainly contains blastula method, nuclear transplantation method and embryo blastomere method.

背景:目前人胚胎干细胞建系的技术路线主要有囊胚法、核移植法和胚胎单卵裂球法,但由于破坏胚胎,涉及诸多伦理问题。

We studied the optimizaiton, accuracy and reliability of this technique by definition the genotype of five common mutation loci of β-globin gene in the carrier with CD41-42 mutation and that of β-globin gene of normal people's single lymphocyte and single blastomere.

我们采用了一种利用单细胞标本通过二重巢式PCR并结合ARMS技术确定中国人群中广泛的β-地中海贫血突变谱的基因型的策略,通过对含有β-珠蛋白基因CD41-42突变的携带者5个该基因常见突变位点基因型的确定,以及正常人的单个淋巴细胞、单卵裂球的β-珠蛋白基因该5个位点基因型的确定,对这种技术条件的优化、准确性和可靠性进行了研究。

The average amplification efficiency of single blastomere was 89.7% and the average allele drop-out rate was 14.4%.

单个卵裂球平均扩增效率为89.7%,等位基因脱扣(allele drop-out, ADO)率为14.4%。

One embryonic cell can be separated into many blastomere cells. As be the nuclear donor of the embryonic cell nuclear transfer, it can breed the number of eminent species largely, increase the number of endangered animals, supply large number of supply same genotype animals, improve the efficiency of gene transfer and sex control and offer same genotype apparatus or organ for human transplant.

一个早期胚胎细胞可以分离多个卵裂球,以此作为核供体进行胚胎细胞核移植,可以大量扩繁优良品种的数量,增加濒危动物的数量,为科学研究提供大量遗传同质的动物,提高转基因和性别控制技术的效率,提供基因型相同的器官或组织供人类移植。

Methods The high frequency mutation region G380R of fibroblast growth factor receptor 3(FGFR3) gene was amplified by nested PCR with single lymphocyte and single blastomere.

采用巢式 PCR扩增单淋巴细胞及单卵裂球的成纤维细胞生长因子受体 3基因的高发突变位点 G380 R区域,用限制酶 Bfm 消化 PCR产物,10 %聚丙烯酰胺凝胶电泳检测。

Use of a "cloning" procedure known as "artifical twinning" or "blastomere splitting" to create several genetically identical embryos from one, so long as the resulting embryos are experimented on and discarded instead of being transferred to the womb.

使用"克隆"程序称为"人工配对"或"卵裂球分裂"制造基因相同的胚胎数由一、只要是试行并导致胚胎被丢弃而不移送的子宫。

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