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In mouse, the position of first polar body (PB1) and MⅡ nucleus or spindle is moveable and thus it is difficult to enucleate beneath the PB1. In this study, the 3% sucrose treatment was prepared for enucleation.

在小鼠的卵母细胞中,第一极体(PB1)与MⅡ期核或纺缍体的位置是不固定的,因此导致了去核的困难。

The polar and egg nuclei of the female gametophyte are genetically identical.

雌配子体中的极核和卵核遗传上是同一的。

Two polar nuclei move to the micropylar end before sperm is released in a gap between the egg cell and central cell and subsequently taking off cytoplasm one after another. The sperm nucleus moving to the egg nucleus runs faster than the one to the polar nuclei. It fuses with the polar nuclei that are moving towards the antipodal cells at the chalazal end.

精子释放前,两极核移向卵细胞的合点端;两精子释放于卵细胞与中央细胞的间隙后,先后脱去细胞质,然后分别移向卵核和极核,移向卵核的精核快于移向极核的精核;精核与两极核在向反足细胞团方向移动的过程中完成雌雄核融合。

Two polar nuclei move to the micropylar end before sperm is released in a gap between the egg cell and central cell and subsequently taking off cytoplasm one after another. The sperm nucleus moving to the egg nucleus runs faster than the one to the polar nuclei. It fuses with the polar nuclei that are moving towards the antipodal cells at the chalazal end, Images showed the fusion process of male and female nuclei and polyspermous fertilization.

精子释放前,两极核移向卵细胞的合点端;两精子释放于卵细胞与中央细胞的间隙后,先后脱去细胞质,然后分别移向卵核和极核,移向卵核的精核快于移向极核的精核;精核与两极核在向反足细胞团方向移动的过程中完成雌雄核融合。

The cloning of mammals by somatic cell nuclear transfer requires epigenetic reprogramming of the differentiated state of donor cell to a totipotent, embryonic ground state.

由于供体核支持整个克隆动物的发育,所以当供体核被移入去核的卵母细胞后,必需进行重编程,回到胚胎发育的起始阶段。

Enucleated metaphase Ⅱ oocyte can support reprogramming of transferred nucleus and further development to term.

许多实验证明去核的MⅡ期卵母细胞可支持移入的核进行重新编程并发育到出生。

Donor cells in manipulation medium contain of 7% ethanol prior to injection were injected directly to enucleated cytoplasts.

供核细胞注核前置于含7%乙醇的操作液中,然后直接注入到去核的卵母细胞质内。

Rachis, pedicels, and hypanthium abaxially pubescent; fruit red or orangish red, obovoid, ovoid, or subglobose, with 2–5 pyrenes.

Rachis,花梗和托杯背面具短柔毛;橙黄红色,倒卵球形,卵球形的果红色或,或近球形,具2-5果核。

Inflorescence 3–11-flowered; petals white; leaf blade ovate-lanceolate to oblong-ovate, abaxially sparsely tomentose; fruit subglobose, with 5 pyrenes.

花序3-11花;花瓣白色卵形披针形叶片卵形的到长圆形,背面稀疏被绒毛;果近球形,具果核。 22 C。 glomerulatus 球花栒子

However, when two maturing oocytes fused and two spindle will form in the big cell, the chromosomes will not intermingle. In the present study, we removed GV from one oocyte and transfer to the perivetelline space of another GV oocyte. After fusion the resulting oocytes which contained two GVs were cultured further in MEM.

在本实验中,我们利用小鼠GV期卵母细胞将一枚卵母细胞的生发泡取出后移至另一未经去核处理的GV期卵母细胞透明带下,经三次电脉冲作用后将融合的含有两个GV的卵母细胞放入MEM成熟培养液中培养,在培养成熟的不同阶段分别收集卵母细胞进行免疫荧光染色观察微管及核的变化。

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Cynanchum Lingtai apricot production in the average weight 65 grams, the brightly-colored fruit, juicy rich, sweet-sour taste, sweet from the nucleolus, when the late Qing Dynasty famous Shaanxi, Gansu provinces, the Qing imperial court Tongzhi tribute for years.

灵台生产的牛心杏平均单果重65克,果实色泽鲜艳,汁多味浓,甜酸适口,离核仁甜,清末时就驰名陕、甘两省,清同治年间曾为朝廷贡品。

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异:红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感,ED90值分别为47.65、71.67和29.17g/hm2;春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感,ED90值分别为96.91、114.20g/hm2;而番茄不敏感。

However, I have an idea.

不过,我有个主意。