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It is the fact that three methionines distributed in the Hsp16.3 will begradually oxidated, in vitro, by hydrogen peroxide(H2O2). When the hsp16. 3 gene was cloned with pMV261 plasmid vector and transfected into Mycobacterium smegmatis, we found that the ability of anti-H2O2 will be decreased greatly among the strains of M. smeg. containing pMV261-hspl6. 3 while compared with the control strains of M. smeg. containing free plasmid vector. But we found that the ability of anti-H2O2 will be increased greatly among the strains of M. smeg. containing pMV261-38kDa while compared with the same control.

尽管Hsp16.3中有三个甲硫氨酸在体外能被过氧化氢(H_2O_2)逐一氧化,我们将Hsp16.3基因克隆到pMV261质粒上,并转染到耻垢分枝杆菌中发现,转染了pMV261-16.3的耻垢分枝杆菌抗过氧化氢的能力大大下降,而对照含pMV261-38kDa的耻垢分枝杆菌抗过氧化氢的能力却有明显的增强,据此,我们认为,Hsp16.3在细胞内可能不具有抗过氧化氢的能力。

Pasteurization, which is often used for milk or waste milk, can be used to reduce bacteria levels and has been shown to be very effective at killing a variety of pathogenic bacteria including Salmonella, E. coli, Mycobacterium avium subspecies paratuberculosis, Mycobacterium californicum, Mycobacterium bovis, and Listeria monocytogenes.

巴氏灭菌常使用于牛奶或牛奶废料,它可以用来降低细菌水平,并已被证明能有效的杀死多种致病细菌,其中包括沙门氏菌、大肠杆菌、副结核分枝杆菌亚种、californicum分枝杆菌、牛分枝杆菌和李斯特菌。

Respectively use chelex 100 resinate,boiling extraction,proteinase K digest/isopropanol precipitation,proteinase K digest/phenol/chloroformextra ction,alkaline lysis,guanidine thiocyanate lysis,guanidine hydrochloride lysis,spin column method to extract Mycobacterium tuberculosis DNA for multiplepolymerase chain eraction.

选择3份含结核分枝杆菌分别为4.325×103、6.857×104、5.356×105 Copies/ml的痰液,分别用Chelex 100树脂法、煮沸法、蛋白酶K消化异丙醇沉淀法、蛋白酶K消化酚/氯仿抽提法、碱裂解法、异硫氰酸胍裂解法、盐酸胍裂解法、离心柱法抽提结核分枝杆菌DNA作多重聚合酶链反应,显色法芯片检测结核分枝杆菌RFP和INH耐药基因。

Two supernumerary spikelet lines and two normal-spiked multispikeletlines of common wheattriticum aestivum L.

玉皮分枝麦和骊英3号分枝麦是稳定的穗分枝型普通小麦品系。

In addition, trichome of AtCK4 transgenic plants has only two branches or no branching; however, that of wild-type plants has three branches.

另外,野生型的叶片表皮毛为三个分枝,而转AtCK4基因植株的表皮毛多为两个分枝,甚至不分枝

The main results were showed as follows:Through screen and identification of morphology in M_2 and M_3, respectively, the mutants with mutative traits of plant were found. In which, 11 mutations were leaf variations, including light-green leaf, deep-green leaf, durative yellowish leaf, temporary yellowish leaf, inlaid yellowish leaf, wrinkly leaf, smooth-edged curly leaf, splitting-shaped curly leaf, peduncular-shaped leaf, round leaf and larger leaf, 12 were mutations on plant type, including excessive branches, lacking branches, highness, dwarf-like, erect posture, reclinate posture, strong stalk, slender stalk, tufty branches, wrinkled plant, purple stalk and hair-covered plant, 14 were flower variations, including dense flowers, light-yellow petals, white petals, white-mosaic petals, wrinkled petales, back-rolled petals, narrow petals, larger petals, smaller petals, apetalous flowers, variable-numbered petals, fertile pistil protraction, sterile pistil protraction and multi-anther, and 5 were physiologic mutants, including genic male sterile, cytoplasmic male sterile, bud-dead, and early and later bloom.

主要结果如下:通过田间M_2筛选和M_3重复鉴定,在田间农艺性状方面共获得浅绿叶色、深绿叶色、转绿黄化叶、持续黄化叶、边缘黄化叶、皱缩叶、光叶型卷叶、裂叶型卷叶、薹叶形叶、宽圆叶和大叶等11种不同的叶部性状突变体;同时还筛选到多分枝、少分枝、高杆、矮杆、株型紧凑、株型松散、粗茎、细茎、丛生分枝、整株皱缩、紫茎、整株被毛等12种植株性状突变体,密花、浅色花瓣、白色花瓣、白斑花瓣、皱瓣、卷瓣、窄瓣、大瓣、小瓣、无瓣、花瓣数目不定、可育型柱头外露、不育型柱头外露和多雄蕊等14种花器性状突变体,以及细胞核雄性不育、细胞质雄性不育、死蕾、早花和迟花等5种生理性状突变体。

The results demonstrated that 22 of 50 were positive by PCR-ELISA method while only 7/50 and 18/50 by antiacid staining and culture respectively, and no positive result was found in 20 sputum specimens without Mycobacterium tuberculosis by the methods; Conclusion The pCR-ELISA method can objectively detect Mycobacterium tuberculosis from clinical specimens with high specificity and sensitivity.

对50份来自结核病人高度怀疑有结核分枝杆菌的标本检测表明,PCR-ELISA检出22份阳性,比抗酸染色法(7/50)、培养法(13/50)和PCR-电泳法(18/50)检出率高,而20份证实无结核分枝杆菌的标本,几种方法检查均为阴性。结论该法可敏感、特异和客观地检测临床标本中的结核分枝杆菌。

In order to investigate new methods to the detection of Mycobacterium tuberculosis and diagnostic value of FQ-PCR in the detection of DNA in Mycobacterium tuberculosis, FQ-PCR was used to detect DNA of Mycobacterium tuberculosis in body fluid from 68 patients with tuberculosis and 68 patients without tuberculosis and it was also compared with the two methods of smear acid-fast stainning and antituberculotic antibody.

为探讨结核分枝杆菌检测的新途径及其FQ-PCR技术在结核分枝杆菌DNA诊断中的实用价值,运用FQ-PCR技术对68例结核病患者和68例非结核病患者体液进行结核分枝杆菌DNA测定,并与涂片抗酸染色及ELISA—抗结核抗体法进行比较。

Observations on the dichotomous leaf venation of Circaeaster agrestis found no intermediate forms of the fusion of vascular bundles between the two completely fused commis-sural veins of anastomoses and the two separate approximate veins, but found different degrees of the vestige of commissural veins .

在星叶草叶二叉分枝脉序中,网结脉中2条完全汇合叶脉与靠近脉中完全分离叶脉间未发现任何形式的维管束汇合的中间类型,相反观察到了网结脉中不同程度的连接脉退化的痕迹,因此网结脉不可能由靠近脉形成;盲脉的出现与齿的退化、网结脉中连接脉的间断及非网结脉由分枝处间断等相关;上述事实表明星叶草叶的二叉分枝脉序应为一减化性状。

Cymes mainly on branched axis, 3 or 4 × dichotomously branched, branches slender and patent; peduncle 1-2 cm, secondary peduncle 5-15 mm; pedicels very short, less than ca. 3 mm or absent.

聚伞花序主要在分枝的轴上,3或者4二歧分枝分枝纤细和开展;花序梗1-2厘米,副花梗5-15毫米;非常短的花梗,少于长约3毫米或无。

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代表父目录,所以这个命令就是执行当前目录下的"toolchain.sh"。

Yes,In fact,I'm on our city ream.

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